Disease relevance of terbium

• Direct kinetic evidence for triplet state energy transfer from Escherichia coli alkaline phosphatase tryptophan 109 to bound terbium [1].
• Terbium-mediated footprinting probes a catalytic conformational switch in the antigenomic hepatitis delta virus ribozyme [2].
• Kinetic effects of terbium ions on muscarinic acetylcholine receptors of murine neuroblastoma cells [3].
• Therefore, terbium is potentially useful in cisplatin combination therapy for breast cancer patients, especially for those patients who have developed resistance to the drug [4].
• Substitution of H2O by D2O shows that Tb-FTP binding to the enzyme nucleotide site is associated with an important dehydration of the terbium ions associated with FTP [5].

High impact information on terbium

• Cation binding to beta 3 (118-131) was demonstrated by terbium luminescence and mass spectroscopy [6].
• Engineering a terbium-binding site into an integral membrane protein for luminescence energy transfer [7].
• Fully functional scallop muscle fibers were prepared such that each myosin molecule contained a terbium-labeled (luminescent donor) RLC on one head and a rhodamine-labeled (acceptor) RLC on the other [8].
• Rat skeletal muscle tissue sections were labeled with both anti-dystrophin monoclonal antibody conjugated to a terbium-based resonance energy transfer donor and anti-actin tetramethylrhodamine phalloidin as an acceptor [9].
• These studies have now been extended to the single-channel level and changes in gating kinetics of GABAA receptor currents caused by 100 microM terbium (Tb3+) are reported [10].

Chemical compound and disease context of terbium

• Although the simultaneous assay using terbium and europium chelates gave lower assay sensitivities for both adenovirus and rotavirus when compared to separate assays using only europium chelates, the sensitivities were found to be at least equal to those of conventional standard antispecies EIA [11].
• Pulmonary toxicity of systemic terbium chloride in mice [12].

Biological context of terbium

• Energy transfer between terbium (III) and cobalt (II) in thermolysin: a new class of metal--metal distance probes [13].
• Terbium (Tb3+), an ion of the lanthanide series that has been used as a fluorescent probe for calcium (Ca2+) binding sites in proteins, binds to the proteins in both solubilized and purified human placental insulin receptor preparations [14].
• Competition between Mg-ATP and Tb-FTP for ATP hydrolysis indicates that terbium-associated nucleotides bind to the catalytic site of the H+-ATPase [15].
• Comparison of terbium (III) luminescence enhancement in mutants of EF hand calcium binding proteins [16].
• Changes in the relative occupancy of metal-binding sites in the profile structure of the sarcoplasmic reticulum membrane induced by phosphorylation of the Ca2+ATPase enzyme in the presence of terbium: a time-resolved, resonance x-ray diffraction study [17].

Anatomical context of terbium

• The interaction of three second-generation anthracycline derivatives with polynucleotides, supercoiled DNA, and calf thymus nucleosomes has been studied by terbium fluorescence measurements and agarose gel electrophoresis [18].
• Ca2+ binding sites of the ryanodine receptor/Ca2+ release channel of sarcoplasmic reticulum. Low affinity binding site(s) as probed by terbium fluorescence [19].
• Acetylcholine receptor-enriched membranes bind 45 terbium cations per receptor [20].
• Terbium binding to ribosomes and ribosomal RNA [21].
• Calcium binding sites of synaptosomes from insect nervous system as probed by trivalent terbium ions [22].

Associations of terbium with other chemical compounds

• The lanthanide terbium is known to interact with guanine and xanthosine to produce high fluorescence enhancement [23].
• Using resonance energy transfer from tryptophan residues, one of the high affinity terbium-binding sites (site A) had a greater affinity than the other two sites (sites B and C) of each protein [24].
• Fluorescence from terbium was not quenched by holmium [25].
• It was concluded that tryptophan in loop position 7 gave optimal luminescence enhancement, and that the additional inclusion of a tyrosine in the loop at positions 2 or 4 could further boost emission from the bound terbium [26].
• The properties of the gadolinium, terbium, and dysprosium complexes of these ligands were examined as a function of pH [27].

Gene context of terbium

• Fluorescence of PON1 complexed to terbium ion shows that at least one tryptophan is close to the calcium binding site [28].
• Terbium binding and isothermal titration calorimetry data reveal that two atoms of Cu2+/Ca2+ bind per subunit of S100A13 [29].
• Europium-labeled 6H10 and terbium-labeled anti-PSA Mab 2E9, selected as the best blocker antibody, were used for dual-label immunodetection in routinely fixed benign (n = 7) and malignant (n = 5) prostate specimens [30].
• In TFE both gastrin and CCK exhibited high affinities for calcium and terbium [31].
• Terbium was displaced from Calb I-II by other lanthanides and calcium [25].

Analytical, diagnostic and therapeutic context of terbium

• The binding of calcium and terbium to purified chick vitamin D-dependent intestinal calcium-binding protein was studied by terbium fluorescence, circular dichroism, and intrinsic protein fluorescence techniques [32].
• Enzymatically amplified time-resolved fluorescence immunoassay with terbium chelates [33].
• This detection strategy has been used in HPLC separations, using either normal- or reversed-phase chromatography with postcolumn addition of detergent and/or terbium, or micellar chromatography with the detergent solution as the mobile phase [34].
• As judged by electron microscopy and x-ray analysis, terbium ions produced electron-dense patches in regular arrays on the outer surface of synaptosomal membranes and induced marked aggregation of synaptic vesicles in isolated terminals [22].
• Synthesis of a terbium fluorescent chelate and its application to time-resolved fluoroimmunoassay [35].

References