Disease relevance of dnaB

• phiX174 DNA-dependent DNA synthesis in vitro: requirement for P1 ban protein in dnaB mutant extracts of Escherichia coli [1].
• Such soluble enzyme fractions were prepared from E. coli strains carrying the prophage mutant P1bac, in which the viral dnaB analog (ban) protein is expressed constitutively, or P1bacban, in which the expression of ban protein is prevented [1].
• The complete nucleotide sequence of the Bacillus subtilis dnaB gene and its flanking regions was determined [2].
• Its insertion into lambda can be selected by plaque formation by using nonsuppressing (sup0) Escherichia coli for amber mutant lambda phage and sup0 dnaB-amber E. coli for nonamber lambda phage [3].
• A dnaB analog specified by bacteriophage P1 [4].

High impact information on dnaB

• Nucleotide sequence of Bacillus subtilis dnaB: a gene essential for DNA replication initiation and membrane attachment [2].
• Biochemical analysis of the altered form of dnaB protein revealed that it was inactive in replication in several purified enzyme systems which involve specific and nonspecific primer formation on single-stranded DNAs, and in replication of plasmids containing the E. coli chromosomal origin [5].
• Sequestering of dnaC protein into inactive complexes may relate to the dominant-lethal phenotype of this dnaB mutant [5].
• With the 52-bp duplex substrate, T antigen movement was only partially (30%) blocked by the tus protein-terB sequence complex in the active orientation, whereas the E. coli dnaB helicase moving 5'----3' was blocked more than 90% by the complex in the active orientation [6].
• Purified dnaA protein, unlike dnaB and dnaC proteins, binds to phospholipid vesicles as judged by analysis on sucrose gradient centrifugation [7].

Biological context of dnaB

• Migration of Escherichia coli dnaB protein on the template DNA strand as a mechanism in initiating DNA replication [8].
• The nucleotide sequence revealed that dnaBI and dnaBII are two functional domains in the single dnaB gene [2].
• Nucleotide sequence and organization of dnaB gene and neighbouring genes on the Bacillus subtilis chromosome [9].
• Similar kinetics were observed in parallel with filament formation induced by incubation of a dnaB mutant of E. coli at the nonpermissive temperature [10].
• After refolding, the fusion protein was purified with a chitin affinity column, and dnaB mini-intein mediated peptide-bond hydrolysis was triggered by shifting the pH in the chitin column to 7.0 at 25 degrees C for 16 h, which led to the release and separation of hBNP from its fusion partner [11].

Associations of dnaB with chemical compounds

• The results indicate that the dnaB protein binds to immobilized nucleotides by means of its ribonucleoside triphosphatase, and that at least the pyrophosphate moiety is essential for adsorption as well as elution of the enzyme [12].

Analytical, diagnostic and therapeutic context of dnaB

• Escherichia coli dnaB protein. Affinity chromatography on immobilized nucleotides [12].
• By use of PCR, the dnaB genes from the classical temperature-sensitive dnaB mutants PC8 (dnaB8), RS162 (dnaB252), CR34/454 (dnaB454), HfrH165/70 (dnaB70), and CR34/43 (dnaB43) were isolated [13].

References