The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

Links

 

Gene Review

CENPA  -  centromere protein A

Homo sapiens

Synonyms: CENP-A, CenH3, Centromere autoantigen A, Centromere protein A, Histone H3-like centromeric protein A
 
 
Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.
 

Disease relevance of CENPA

 

High impact information on CENPA

  • Mis16 and Mis18 are required for CENP-A loading and histone deacetylation at centromeres [6].
  • RNAi knockdown in human cells shows that Mis16 function is conserved as RbAp48 and RbAp46 are both required for localization of human CENP-A [6].
  • Many of these proteins, such as the centromeric histone variant CENP-A, and entire subcomplexes, such as the Ndc80(Hec1) complex, are conserved from yeast to humans despite the diverse nature of the DNA sequences on which they assemble [7].
  • We now show in human cells and in yeast that depletion of CENP-A is lethal, but recruitment of normal levels of kinetochore proteins, centromere-generated mitotic checkpoint signaling, chromosome segregation, and viability can be rescued by histone H3 carrying the CATD [8].
  • The CENP-A NAC is essential, as disruption of the complex causes errors of chromosome alignment and segregation that preclude cell survival despite continued centromere-derived mitotic checkpoint signalling [9].
 

Biological context of CENPA

 

Anatomical context of CENPA

 

Associations of CENPA with chemical compounds

  • However, long-term culture in selective medium, or short-term treatment with the histone deacetylase inhibitor Trichostatin A (TSA), promoted the re-assembly of CENPA, -B and -C at the YAC site and the release of minichromosomes containing the YAC integration site [16].
  • Mammalian centromere function depends upon a specialized chromatin organization where distinct domains of CENP-A and dimethyl K4 histone H3, forming centric chromatin, are uniquely positioned on or near the surface of the chromosome [17].
  • Consistent with this finding, depletion of BubR1 increases phosphorylation of CENP-A, a kinetochore-specific Aurora kinase substrate [18].
  • CENP-A and heterochromatin were assembled over noncentromeric DNA, including the gene blasticidin, into nonoverlapping domains [19].
  • Blasticidin transcripts were enriched at sites of CENP-A binding but not at H3 methylated at lysine 9, indicating that formation of CEN chromatin within a repetitive DNA environment does not preclude gene expression [19].
  • Tricostatin A, an inhibitor to histone deacetylase, suppresses the loss of CENP-A recruitment to centromeres in hMis18alpha RNAi cells [20].
 

Physical interactions of CENPA

 

Co-localisations of CENPA

 

Other interactions of CENPA

 

Analytical, diagnostic and therapeutic context of CENPA

References

  1. Overexpression and mistargeting of centromere protein-A in human primary colorectal cancer. Tomonaga, T., Matsushita, K., Yamaguchi, S., Oohashi, T., Shimada, H., Ochiai, T., Yoda, K., Nomura, F. Cancer Res. (2003) [Pubmed]
  2. A novel centromere monospecific serum to a human autoepitope on the histone H3-like protein CENP-A. Valdivia, M.M., Figueroa, J., Iglesias, C., Ortíz, M. FEBS Lett. (1998) [Pubmed]
  3. Development of a CENP-A/CENP-B-specific immune response in a patient with systemic sclerosis. Mahler, M., Mierau, R., Genth, E., Blüthner, M. Arthritis Rheum. (2002) [Pubmed]
  4. Molecular cloning and sequence analysis of hamster CENP-A cDNA. Figueroa, J., Pendón, C., Valdivia, M.M. BMC Genomics (2002) [Pubmed]
  5. Detection of anticentromere antibodies using recombinant human CENP-A protein. Sun, D., Martinez, A., Sullivan, K.F., Sharp, G.C., Hoch, S.O. Arthritis Rheum. (1996) [Pubmed]
  6. Mis16 and Mis18 are required for CENP-A loading and histone deacetylation at centromeres. Hayashi, T., Fujita, Y., Iwasaki, O., Adachi, Y., Takahashi, K., Yanagida, M. Cell (2004) [Pubmed]
  7. Kinetochore-spindle microtubule interactions during mitosis. Kline-Smith, S.L., Sandall, S., Desai, A. Curr. Opin. Cell Biol. (2005) [Pubmed]
  8. Centromere identity maintained by nucleosomes assembled with histone H3 containing the CENP-A targeting domain. Black, B.E., Jansen, L.E., Maddox, P.S., Foltz, D.R., Desai, A.B., Shah, J.V., Cleveland, D.W. Mol. Cell (2007) [Pubmed]
  9. The human CENP-A centromeric nucleosome-associated complex. Foltz, D.R., Jansen, L.E., Black, B.E., Bailey, A.O., Yates, J.R., Cleveland, D.W. Nat. Cell Biol. (2006) [Pubmed]
  10. CENP-A is phosphorylated by Aurora B kinase and plays an unexpected role in completion of cytokinesis. Zeitlin, S.G., Shelby, R.D., Sullivan, K.F. J. Cell Biol. (2001) [Pubmed]
  11. Mapping the assembly pathways that specify formation of the trilaminar kinetochore plates in human cells. Liu, S.T., Rattner, J.B., Jablonski, S.A., Yen, T.J. J. Cell Biol. (2006) [Pubmed]
  12. CENP-A phosphorylation by Aurora-A in prophase is required for enrichment of Aurora-B at inner centromeres and for kinetochore function. Kunitoku, N., Sasayama, T., Marumoto, T., Zhang, D., Honda, S., Kobayashi, O., Hatakeyama, K., Ushio, Y., Saya, H., Hirota, T. Dev. Cell (2003) [Pubmed]
  13. CENP-G: a new centromeric protein that is associated with the alpha-1 satellite DNA subfamily. He, D., Zeng, C., Woods, K., Zhong, L., Turner, D., Busch, R.K., Brinkley, B.R., Busch, H. Chromosoma (1998) [Pubmed]
  14. Centromere/kinetochore localization of human centromere protein A (CENP-A) exogenously expressed as a fusion to green fluorescent protein. Sugimoto, K., Fukuda, R., Himeno, M. Cell Struct. Funct. (2000) [Pubmed]
  15. Relevance of histone acetylation and replication timing for deposition of centromeric histone CENP-A. Ouspenski, I.I., Van Hooser, A.A., Brinkley, B.R. Exp. Cell Res. (2003) [Pubmed]
  16. Epigenetic assembly of centromeric chromatin at ectopic alpha-satellite sites on human chromosomes. Nakano, M., Okamoto, Y., Ohzeki, J., Masumoto, H. J. Cell. Sci. (2003) [Pubmed]
  17. H2A.Z contributes to the unique 3D structure of the centromere. Greaves, I.K., Rangasamy, D., Ridgway, P., Tremethick, D.J. Proc. Natl. Acad. Sci. U.S.A. (2007) [Pubmed]
  18. The human mitotic checkpoint protein BubR1 regulates chromosome-spindle attachments. Lampson, M.A., Kapoor, T.M. Nat. Cell Biol. (2005) [Pubmed]
  19. Human centromeric chromatin is a dynamic chromosomal domain that can spread over noncentromeric DNA. Lam, A.L., Boivin, C.D., Bonney, C.F., Rudd, M.K., Sullivan, B.A. Proc. Natl. Acad. Sci. U.S.A. (2006) [Pubmed]
  20. Priming of centromere for CENP-A recruitment by human hMis18alpha, hMis18beta, and M18BP1. Fujita, Y., Hayashi, T., Kiyomitsu, T., Toyoda, Y., Kokubu, A., Obuse, C., Yanagida, M. Dev. Cell (2007) [Pubmed]
  21. CENP-A, -B, and -C chromatin complex that contains the I-type alpha-satellite array constitutes the prekinetochore in HeLa cells. Ando, S., Yang, H., Nozaki, N., Okazaki, T., Yoda, K. Mol. Cell. Biol. (2002) [Pubmed]
  22. The constitutive centromere component CENP-50 is required for recovery from spindle damage. Minoshima, Y., Hori, T., Okada, M., Kimura, H., Haraguchi, T., Hiraoka, Y., Bao, Y.C., Kawashima, T., Kitamura, T., Fukagawa, T. Mol. Cell. Biol. (2005) [Pubmed]
  23. Assembly of additional heterochromatin distinct from centromere-kinetochore chromatin is required for de novo formation of human artificial chromosome. Nakashima, H., Nakano, M., Ohnishi, R., Hiraoka, Y., Kaneda, Y., Sugino, A., Masumoto, H. J. Cell. Sci. (2005) [Pubmed]
  24. Centromere targeting of the chromosomal passenger complex requires a ternary subcomplex of Borealin, Survivin, and the N-terminal domain of INCENP. Klein, U.R., Nigg, E.A., Gruneberg, U. Mol. Biol. Cell (2006) [Pubmed]
  25. Human centromere protein A (CENP-A) can replace histone H3 in nucleosome reconstitution in vitro. Yoda, K., Ando, S., Morishita, S., Houmura, K., Hashimoto, K., Takeyasu, K., Okazaki, T. Proc. Natl. Acad. Sci. U.S.A. (2000) [Pubmed]
  26. Assembly of CENP-A into centromeric chromatin requires a cooperative array of nucleosomal DNA contact sites. Shelby, R.D., Vafa, O., Sullivan, K.F. J. Cell Biol. (1997) [Pubmed]
  27. CENP-B box is required for de novo centromere chromatin assembly on human alphoid DNA. Ohzeki, J., Nakano, M., Okada, T., Masumoto, H. J. Cell Biol. (2002) [Pubmed]
  28. The centromere protein CENP-B behaves as a microtubule-associated protein. Armas-Portela, R., Kremer, L., Avila, J. Acta Histochem. Suppl. (1991) [Pubmed]
 
WikiGenes - Universities