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Gene Review

TGOLN2  -  trans-golgi network protein 2

Homo sapiens

Synonyms: TGN38, TGN38 homolog, TGN46, TGN48, TGN51, ...
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Disease relevance of TGOLN2


High impact information on TGOLN2

  • TGN38/41 is a heterodimeric integral membrane protein that cycles between the trans Golgi network and the cell surface [4].
  • Overexpression of Rab11wt, Rab11Q70L, and Rab11S25N also inhibited STxB and TGN38 transport from endosomes to the TGN [5].
  • We also studied transport from endosomes to the Golgi apparatus using the Shiga toxin B subunit (STxB) and TGN38 as reporter molecules [5].
  • Overexpression of both Rab11 wild-type (Rab11wt) and mutants altered the localization of the transferrrin receptor (TfR), internalized Tf, the STxB, and TGN38 [5].
  • Mutational analysis revealed that Asp176 and Trp421 of mu2 are involved in the interaction with internalization motifs of TGN38 and epidermal growth factor (EGF) receptor [6].

Biological context of TGOLN2

  • Moreover, reverse transcription-polymerase chain reaction analysis of hTGN46 transcripts and genomic analysis of a cDNA deposited as an expressed sequence tag in dbEST Data Base revealed that additional cDNAs exist that are produced by alternate usage of 3'-splice sites of intron III [7].
  • Whereas rat TGN38 contains 6 tandem repeats of an 8mer, both primate proteins possess 14 tandem repeats of a 14mer sequence [8].
  • Direct interaction of the trans-Golgi network membrane protein, TGN38, with the F-actin binding protein, neurabin [9].
  • After arrest of the biosynthetic protein transport at 20 degrees C, fluorescent hCgB-GFP colocalized with TGN38, a marker of the TGN [10].
  • We have undertaken a series of experiments designed to address this hypothesis, and, in so doing, have partially characterised the glycosylation status of the lumenal domain of TGN38 [11].

Anatomical context of TGOLN2


Associations of TGOLN2 with chemical compounds

  • We have used a combination of yeast two-hybrid and in vitro protein interaction assays to show that this interaction is dependent on the serine (but not tyrosine) residue of the known TGN38 trafficking motif [9].
  • Specificity of interaction between adaptor-complex medium chains and the tyrosine-based sorting motifs of TGN38 and lgp120 [13].
  • Under physiological conditions when TGN38 is not overexpressed, it interacts with integrin beta 1 [14].
  • The luminal domain of TGN38 interacts with integrin beta 1 and is involved in its trafficking [14].
  • The TGN38 glycoprotein contains two non-overlapping signals that mediate localization to the trans-Golgi network [15].

Physical interactions of TGOLN2

  • Confocal analysis revealed that these vesicles were devoid of TGN38 and of Texas Red-coupled transferrin and cathepsin D, markers of the endosomal/lysosomal pathway [10].

Co-localisations of TGOLN2


Other interactions of TGOLN2


Analytical, diagnostic and therapeutic context of TGOLN2


  1. Cytoplasmic tail motifs mediate endoplasmic reticulum localization and export of transmembrane reporters in the protozoan parasite Toxoplasma gondii. Hoppe, H.C., Joiner, K.A. Cell. Microbiol. (2000) [Pubmed]
  2. Redistribution of cellular and herpes simplex virus proteins from the trans-golgi network to cell junctions without enveloped capsids. Wisner, T.W., Johnson, D.C. J. Virol. (2004) [Pubmed]
  3. Actin and microtubule regulation of trans-Golgi network architecture, and copper-dependent protein transport to the cell surface. Cobbold, C., Coventry, J., Ponnambalam, S., Monaco, A.P. Mol. Membr. Biol. (2004) [Pubmed]
  4. TGN38/41: a molecule on the move. Stanley, K.K., Howell, K.E. Trends Cell Biol. (1993) [Pubmed]
  5. Rab11 regulates the compartmentalization of early endosomes required for efficient transport from early endosomes to the trans-golgi network. Wilcke, M., Johannes, L., Galli, T., Mayau, V., Goud, B., Salamero, J. J. Cell Biol. (2000) [Pubmed]
  6. Inhibition of the receptor-binding function of clathrin adaptor protein AP-2 by dominant-negative mutant mu2 subunit and its effects on endocytosis. Nesterov, A., Carter, R.E., Sorkina, T., Gill, G.N., Sorkin, A. EMBO J. (1999) [Pubmed]
  7. Molecular cloning and expression of a novel human trans-Golgi network glycoprotein, TGN51, that contains multiple tyrosine-containing motifs. Kain, R., Angata, K., Kerjaschki, D., Fukuda, M. J. Biol. Chem. (1998) [Pubmed]
  8. Primate homologues of rat TGN38: primary structure, expression and functional implications. Ponnambalam, S., Girotti, M., Yaspo, M.L., Owen, C.E., Perry, A.C., Suganuma, T., Nilsson, T., Fried, M., Banting, G., Warren, G. J. Cell. Sci. (1996) [Pubmed]
  9. Direct interaction of the trans-Golgi network membrane protein, TGN38, with the F-actin binding protein, neurabin. Stephens, D.J., Banting, G. J. Biol. Chem. (1999) [Pubmed]
  10. Microtubule-dependent transport of secretory vesicles visualized in real time with a GFP-tagged secretory protein. Wacker, I., Kaether, C., Krömer, A., Migala, A., Almers, W., Gerdes, H.H. J. Cell. Sci. (1997) [Pubmed]
  11. Characterisation of the lumenal domain of TGN38 and effects of elevated expression of TGN38 on glycoprotein secretion. Lee, S.S., Banting, G. Eur. J. Cell Biol. (2002) [Pubmed]
  12. Lumenal and transmembrane domains play a role in sorting type I membrane proteins on endocytic pathways. Reaves, B.J., Banting, G., Luzio, J.P. Mol. Biol. Cell (1998) [Pubmed]
  13. Specificity of interaction between adaptor-complex medium chains and the tyrosine-based sorting motifs of TGN38 and lgp120. Stephens, D.J., Banting, G. Biochem. J. (1998) [Pubmed]
  14. The luminal domain of TGN38 interacts with integrin beta 1 and is involved in its trafficking. Wang, J., Howell, K.E. Traffic (2000) [Pubmed]
  15. The TGN38 glycoprotein contains two non-overlapping signals that mediate localization to the trans-Golgi network. Ponnambalam, S., Rabouille, C., Luzio, J.P., Nilsson, T., Warren, G. J. Cell Biol. (1994) [Pubmed]
  16. Intracellular trafficking and activation of the furin proprotein convertase: localization to the TGN and recycling from the cell surface. Molloy, S.S., Thomas, L., VanSlyke, J.K., Stenberg, P.E., Thomas, G. EMBO J. (1994) [Pubmed]
  17. Profilin I attached to the Golgi is required for the formation of constitutive transport vesicles at the trans-Golgi network. Dong, J., Radau, B., Otto, A., Müller, E., Lindschau, C., Westermann, P. Biochim. Biophys. Acta (2000) [Pubmed]
  18. GRIP domain-mediated targeting of two new coiled-coil proteins, GCC88 and GCC185, to subcompartments of the trans-Golgi network. Luke, M.R., Kjer-Nielsen, L., Brown, D.L., Stow, J.L., Gleeson, P.A. J. Biol. Chem. (2003) [Pubmed]
  19. The class II phosphoinositide 3-kinase PI3K-C2alpha is concentrated in the trans-Golgi network and present in clathrin-coated vesicles. Domin, J., Gaidarov, I., Smith, M.E., Keen, J.H., Waterfield, M.D. J. Biol. Chem. (2000) [Pubmed]
  20. Mammalian GRIP domain proteins differ in their membrane binding properties and are recruited to distinct domains of the TGN. Derby, M.C., van Vliet, C., Brown, D., Luke, M.R., Lu, L., Hong, W., Stow, J.L., Gleeson, P.A. J. Cell. Sci. (2004) [Pubmed]
  21. Aquaporin-2 is retrieved to the apical storage compartment via early endosomes and phosphatidylinositol 3-kinase-dependent pathway. Tajika, Y., Matsuzaki, T., Suzuki, T., Aoki, T., Hagiwara, H., Kuwahara, M., Sasaki, S., Takata, K. Endocrinology (2004) [Pubmed]
  22. Brefeldin A (BFA) inhibits basolateral membrane (BLM) delivery and dimerization of transcobalamin II receptor in human intestinal epithelial Caco-2 cells. BFA effects on BLM cholesterol content. Bose, S., Chapin, S.J., Seetharam, S., Feix, J., Mostov, K.E., Seetharam, B. J. Biol. Chem. (1998) [Pubmed]
  23. A role for GRIP domain proteins and/or their ligands in structure and function of the trans Golgi network. Yoshino, A., Bieler, B.M., Harper, D.C., Cowan, D.A., Sutterwala, S., Gay, D.M., Cole, N.B., McCaffery, J.M., Marks, M.S. J. Cell. Sci. (2003) [Pubmed]
  24. Characterization of the Chlamydia trachomatis vacuole and its interaction with the host endocytic pathway in HeLa cells. van Ooij, C., Apodaca, G., Engel, J. Infect. Immun. (1997) [Pubmed]
  25. The trans-Golgi network can be dissected structurally and functionally from the cisternae of the Golgi complex by brefeldin A. Ladinsky, M.S., Howell, K.E. Eur. J. Cell Biol. (1992) [Pubmed]
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