The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

Links

 

Gene Review

GNA13  -  guanine nucleotide binding protein (G...

Homo sapiens

Synonyms: G alpha-13, G-protein subunit alpha-13, G13, Guanine nucleotide-binding protein subunit alpha-13, MGC46138
 
 
Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.
 

Disease relevance of GNA13

  • The alpha-subunits of the G-proteins G12 and G13 were expressed with a baculovirus system in insect cells and analysed for acylation [1].
  • These genetic and proliferative effects can be induced by activation of receptors that signal through heterotrimeric GTP-binding proteins (G-proteins) of the Gq family, pertussis toxin-sensitive Gi/Go proteins, Gs, or G12/G13 [2].
  • 2. Normal median nerve SEPs (P20-N30, N20-P30, and P25-N35) were recorded from the cortical surface of a patient (G13) undergoing a cortical excision for relief of focal seizures [3].
  • A series of viral reassortants was prepared between equine rotaviruses H1 (G5), H2 (G3), and L338 (G13) and human rotavirus ST3 (G4) [4].
  • Concomitant regulation of Ca2+ mobilization and G13 expression in human erythroleukemia cells [5].
 

High impact information on GNA13

  • Recombinant p115 RhoGEF and a fusion protein containing the amino terminus of p115 had specific activity as GTPase activating proteins toward the alpha subunits of the G proteins G12 and G13, but not toward members of the Gs, Gi, or Gq subfamilies of Galpha proteins [6].
  • Here, we show that RGS16 inhibits G alpha 13-mediated, RhoA-dependent reversal of stellation and SRE activation [7].
  • These results elucidate a new mechanism whereby a classical RGS protein regulates G alpha 13-mediated signal transduction independently of the RGS box [7].
  • In conclusion, G2A represents a family of GPCRs expressed in lymphocytes that may link diverse stimuli to cytoskeletal reorganization and transcriptional activation through a pathway involving G alpha 13 and RhoA [8].
  • In membranes of human thyroid gland, we immunologically identified alpha subunits of the G proteins Gs short, Gs long, Gi1, Gi2, Gi3, G(o) (Go2 and another form of Go, presumably Go1), Gq, G11, G12, and G13 [9].
 

Chemical compound and disease context of GNA13

  • However, other pertussis toxin-insensitive G proteins such as Gq, G12 and G13 were unable to interact with the melatonin receptor [10].
 

Biological context of GNA13

 

Anatomical context of GNA13

  • Using subtype-specific antisera, we were able to identify the recently described alpha subunits of G12 and G13 in platelet membranes as 43-kDa proteins [12].
  • Treatment of K562 cells with DMSO reduced the levels of thrombin receptor mRNA, without simultaneous changes in the expression of G12 alpha and G13 alpha [15].
  • Consistent with this observation, ADP-ribosylation experiments revealed the presence of two PT substrates which co-migrated with human erythrocyte G12 alpha and G13 alpha [15].
  • Characterization of G alpha 13-dependent plasma membrane recruitment of p115RhoGEF [16].
  • Studies with the constitutively activated mutants of G alpha 12 and G alpha 13 have indicated that they stimulate mitogenic signaling pathways leading to the oncogenic transformation of fibroblast cell lines [17].
 

Associations of GNA13 with chemical compounds

  • Effective activation of G12 and G13 via the thromboxane A2 and the thrombin receptors was not dependent on the presence of GDP [12].
  • GTP gamma S significantly (P < .05) decreased the affinity of the receptor for 125I-BOP in COS-7 cell membranes coexpressing HEL-TXR and G alpha 13 to a value comparable to HEL-TXA2 receptor alone [18].
  • In separate experiments, it was found that the TXA2 receptor agonist U46619 stimulated [35S]guanosine 5'-O-(3-thiotriphosphate) incorporation into G13 alpha-subunit [19].
  • We were interested especially in the potential of the isoprostane 8-iso-prostaglandin F (8-iso-PGF2alpha), among other ligands examined, to activate G12 and G13 through TPalpha explicitly [20].
  • Fatty acid analysis after labeling with [3H]palmitic acid showed that palmitate represents the predominant fatty acid linked to G alpha 12 and G alpha 13 [1].
 

Physical interactions of GNA13

  • Using in vitro binding and coimmunoprecipitation assays, we have shown that only activated G alpha 13 binds to AKAP110, suggesting a potential role for AKAP110 as a G alpha subunit effector protein [21].
 

Regulatory relationships of GNA13

  • G alpha 12 required a higher Mg2+ concentration for AlF4- -induced dissociation from immobilized G beta gamma than did G alpha 13 [22].
  • Importantly, truncated G alpha 13 retained its ability to stimulate apoptosis signal-regulated kinase ASK1 and c-Jun terminal kinase, JNK [23].
 

Other interactions of GNA13

 

Analytical, diagnostic and therapeutic context of GNA13

References

  1. The alpha-subunits of G-proteins G12 and G13 are palmitoylated, but not amidically myristoylated. Veit, M., Nürnberg, B., Spicher, K., Harteneck, C., Ponimaskin, E., Schultz, G., Schmidt, M.F. FEBS Lett. (1994) [Pubmed]
  2. G protein-coupled receptors and signaling pathways regulating growth responses. Post, G.R., Brown, J.H. FASEB J. (1996) [Pubmed]
  3. Cortical somatosensory evoked potentials. II. Effects of excision of somatosensory or motor cortex in humans and monkeys. Allison, T., Wood, C.C., McCarthy, G., Spencer, D.D. J. Neurophysiol. (1991) [Pubmed]
  4. Serological and genomic characterization of equine rotavirus VP4 proteins identifies three different P serotypes. Isa, P., Snodgrass, D.R. Virology (1994) [Pubmed]
  5. Concomitant regulation of Ca2+ mobilization and G13 expression in human erythroleukemia cells. Michel, M.C. Eur. J. Pharmacol. (1998) [Pubmed]
  6. p115 RhoGEF, a GTPase activating protein for Galpha12 and Galpha13. Kozasa, T., Jiang, X., Hart, M.J., Sternweis, P.M., Singer, W.D., Gilman, A.G., Bollag, G., Sternweis, P.C. Science (1998) [Pubmed]
  7. RGS16 inhibits signalling through the G alpha 13-Rho axis. Johnson, E.N., Seasholtz, T.M., Waheed, A.A., Kreutz, B., Suzuki, N., Kozasa, T., Jones, T.L., Brown, J.H., Druey, K.M. Nat. Cell Biol. (2003) [Pubmed]
  8. Direct genetic demonstration of G alpha 13 coupling to the orphan G protein-coupled receptor G2A leading to RhoA-dependent actin rearrangement. Kabarowski, J.H., Feramisco, J.D., Le, L.Q., Gu, J.L., Luoh, S.W., Simon, M.I., Witte, O.N. Proc. Natl. Acad. Sci. U.S.A. (2000) [Pubmed]
  9. The human thyrotropin receptor: a heptahelical receptor capable of stimulating members of all four G protein families. Laugwitz, K.L., Allgeier, A., Offermanns, S., Spicher, K., Van Sande, J., Dumont, J.E., Schultz, G. Proc. Natl. Acad. Sci. U.S.A. (1996) [Pubmed]
  10. Stimulation of cAMP accumulation by the cloned Xenopus melatonin receptor through Gi and Gz proteins. Yung, L.Y., Tsim, S.T., Wong, Y.H. FEBS Lett. (1995) [Pubmed]
  11. Expression of GTP-binding protein alpha subunits in human thymocytes. Kabouridis, P.S., Waters, S.T., Escobar, S., Stanners, J., Tsoukas, C.D. Mol. Cell. Biochem. (1995) [Pubmed]
  12. G proteins of the G12 family are activated via thromboxane A2 and thrombin receptors in human platelets. Offermanns, S., Laugwitz, K.L., Spicher, K., Schultz, G. Proc. Natl. Acad. Sci. U.S.A. (1994) [Pubmed]
  13. Galpha12 and galpha13 are phosphorylated during platelet activation. Offermanns, S., Hu, Y.H., Simon, M.I. J. Biol. Chem. (1996) [Pubmed]
  14. Coupling interaction between thromboxane A2 receptor and alpha-13 subunit of guanine nucleotide-binding protein. Chou, K.C. J. Proteome Res. (2005) [Pubmed]
  15. Ca2+ signalling in K562 human erythroleukaemia cells: effect of dimethyl sulphoxide and role of G-proteins in thrombin- and thromboxane A2-activated pathways. Thomas, C.P., Dunn, M.J., Mattera, R. Biochem. J. (1995) [Pubmed]
  16. Characterization of G alpha 13-dependent plasma membrane recruitment of p115RhoGEF. Bhattacharyya, R., Wedegaertner, P.B. Biochem. J. (2003) [Pubmed]
  17. Signaling by the G12 class of G proteins. Dhanasekaran, N., Dermott, J.M. Cell. Signal. (1996) [Pubmed]
  18. Characterization of the cloned HEL cell thromboxane A2 receptor: evidence that the affinity state can be altered by G alpha 13 and G alpha q. Allan, C.J., Higashiura, K., Martin, M., Morinelli, T.A., Kurtz, D.T., Geoffroy, O., Meier, G.P., Gettys, T.W., Halushka, P.V. J. Pharmacol. Exp. Ther. (1996) [Pubmed]
  19. Identification of Galpha13 as one of the G-proteins that couple to human platelet thromboxane A2 receptors. Djellas, Y., Manganello, J.M., Antonakis, K., Le Breton, G.C. J. Biol. Chem. (1999) [Pubmed]
  20. The G12 family of G proteins as a reporter of thromboxane A2 receptor activity. Zhang, L., DiLizio, C., Kim, D., Smyth, E.M., Manning, D.R. Mol. Pharmacol. (2006) [Pubmed]
  21. Interaction of heterotrimeric G13 protein with an A-kinase-anchoring protein 110 (AKAP110) mediates cAMP-independent PKA activation. Niu, J., Vaiskunaite, R., Suzuki, N., Kozasa, T., Carr, D.W., Dulin, N., Voyno-Yasenetskaya, T.A. Curr. Biol. (2001) [Pubmed]
  22. Distinct biochemical properties of the native members of the G12 G-protein subfamily. Characterization of G alpha 12 purified from rat brain. Harhammer, R., Nürnberg, B., Harteneck, C., Leopoldt, D., Exner, T., Schultz, G. Biochem. J. (1996) [Pubmed]
  23. G alpha 13-mediated transformation and apoptosis are permissively dependent on basal ERK activity. Adarichev, V.A., Vaiskunaite, R., Niu, J., Balyasnikova, I.V., Voyno-Yasenetskaya, T.A. Am. J. Physiol., Cell Physiol. (2003) [Pubmed]
  24. Functional consequences of G alpha 13 mutations that disrupt interaction with p115RhoGEF. Grabocka, E., Wedegaertner, P.B. Oncogene (2005) [Pubmed]
  25. Regulation of apoptosis by alpha-subunits of G12 and G13 proteins via apoptosis signal-regulating kinase-1. Berestetskaya, Y.V., Faure, M.P., Ichijo, H., Voyno-Yasenetskaya, T.A. J. Biol. Chem. (1998) [Pubmed]
 
WikiGenes - Universities