Disease relevance of Aspm

• Differential expression of MARCKS and other calmodulin-binding protein kinase C substrates in cultured neuroblastoma and glioma cells [1].

High impact information on Aspm

• An F-actin- and calmodulin-binding protein from isolated intestinal brush borders has a morphology related to spectrin [2].
• This desmosomal calmodulin-binding protein, which we have termed keratocalmin, may be involved in the calcium-regulated assembly of desmosomes [3].
• CAP23 is a major cortical cytoskeleton-associated and calmodulin binding protein that is widely and abundantly expressed during development, maintained in selected brain structures in the adult, and reinduced during nerve regeneration [4].
• Thus, calmodulin and the heat-labile calmodulin-binding protein may play a role in the nervous system at the site of neurotransmitter action and at the level of microtubular function [5].
• Concomitant with the resulting increase in abventricular cells in the ventricular zone, a larger proportion of NE cell progeny is found in the neuronal layer, implying a reduction in the number of NE progenitor cells upon Aspm knock-down relative to control [6].

Biological context of Aspm

• Upon RNA interference in telencephalic NE cells, Aspm mRNA is reduced, mitotic spindle poles lack Aspm protein, and the cleavage plane of NE cells is less frequently oriented perpendicular to the ventricular surface of the neuroepithelium [6].
• Both aspects of the phenotype induced by this cDNA product, which we term Sha1 (for spindle and hydroxyurea checkpoint abnormal), were suppressed by simultaneous overexpression of calmodulin [7].
• Polyclonal antibodies raised against bovine brain P-57, a neural-specific calmodulin-binding protein, were used to isolate murine cDNAs encoding P-57 from murine brain cDNA libraries in the expression vector lambda gt 11 [8].
• The gene consists of 12 exons and 11 introns and is predicted to encode both beta and alpha forms of CaM kinase IV as well as the testis-specific calmodulin-binding protein calspermin [9].
• Furthermore, a 67 kDa binding protein was the major calmodulin binding protein from human stomach and ileum as determined by ion exchange and calmodulin affinity chromatography [10].

Anatomical context of Aspm

• Here we investigate the role of Aspm in mouse embryonic neuroepithelial (NE) cells, the primary stem and progenitor cells of the mammalian brain [6].
• Aspm specifically maintains symmetric proliferative divisions of neuroepithelial cells [6].
• In the adult, this Calmbp1 transcript was detectable by Northern blot analysis exclusively in testis, ovary and spleen of all organs examined [11].
• We have investigated the expression of Calmbp1 in mid-gestation and late-gestation fetuses and in adult organs of the mouse [11].
• Immunocytochemical localization of calmodulin and a heat-labile calmodulin-binding protein (CaM-BP80) in basal ganglia of mouse brain [5].

Associations of Aspm with chemical compounds

• Characterization of a calmodulin-binding protein that is deficient in trifluoperazine-resistant variants of the macrophage-like cell line J774 [12].
• Its sedimentation constant in glycerol gradients containing calmodulin was dependent upon the relative concentrations of calmodulin and the calmodulin-binding protein [12].

Other interactions of Aspm

• We have shown that interleukin-3 (IL-3)-dependent proliferation of NFS-60 cells is associated with the induction of a specific calmodulin-binding protein of about 68 kD (CaM-BP68) [13].
• To identify the calmodulin-binding protein involved in this process, calmodulin affinity chromatography was performed [14].
• RC3 is a neuronal calmodulin-binding protein and protein kinase C substrate that is thought to play an important regulatory role in synaptic transmission and neuronal plasticity [15].
• Calponin is a smooth muscle specific, actin-, tropomyosin- and calmodulin-binding protein thought to be involved in some way in the regulation or modulation of contraction [16].
• Of the tissues examined, expression of the CaM kinase IV gene is restricted to brain and testis, where transcripts are differentially expressed to produce a kinase in both tissues and a calmodulin-binding protein, calspermin, in testis that lacks a kinase catalytic domain [17].

Analytical, diagnostic and therapeutic context of Aspm

• Immunoassay and immunopurification of macrophage calmodulin-binding protein [18].
• SDS-PAGE on 12.5 per cent gels of eluates obtained from calmodulin-agarose columns, followed by overlay of corresponding western blots with biotinylated calmodulin revealed the presence of a 68 kDa calmodulin binding protein in samples collected either at 28 wk or at full-term of pregnancy [19].

References