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Cyp2b9  -  cytochrome P450, family 2, subfamily b,...

Mus musculus

Synonyms: 16\alphaoh-a, CYPIIB9, Cyp2b, Cyp2b-9, Cytochrome P450 2B9, ...
 
 
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Disease relevance of Cyp2b9

  • To understand the functions of these splice variants in physiological and tumor angiogenesis, we used EIIIB-null and EIIIA-null strains of mice to examine neovascularization of mouse retinas, pancreatic tumors in Rip-Tag transgenic mice, and transplanted melanomas [1].
  • Regression of spontaneously arising, late-stage pancreatic islet cell carcinomas in Rip Tag transgenic mice was also observed [2].
 

High impact information on Cyp2b9

  • Microsomal testosterone 16 alpha-hydroxylase (cytochrome P-450(16) alpha) was purified from the livers of male 129/J mice based on enzyme activity in the eluates from columns of DEAE Bio-Gel A, hydroxylapatite, and isobutyl-Sepharose 4B [3].
  • Genetic regulation of estrogen-dependent repression of female-specific testosterone 16 alpha-hydroxylase (I-P-450(16 alpha) in male mouse liver: murine Ripr locus [4].
  • The distribution of derepression among castrated recombinant inbred strains (9 X A) indicated a close link of a locus repressing I-P-450(16 alpha) in male mice to the Rip locus on chromosome 7 [4].
  • The constitutive expression of phenobarbital-inducible mouse cytochrome P-450 (I-P-450(16 alpha) at the mRNA level and its associated testosterone 16 alpha-hydroxylase activity in liver microsomes was a female characteristic in many inbred mice, including BALB/cJ, A/HeJ, and C57BL/6J [5].
  • Characterization of testosterone 16 alpha-hydroxylase (I-P-450(16) alpha) induced by phenobarbital in mice [6].
 

Biological context of Cyp2b9

 

Anatomical context of Cyp2b9

  • The expression of Cyp2b9 and Cyp2b10 genes was investigated in kidney, liver, and cultured hepatocytes of adult C57BL/6NCrj mice [10].
  • These findings indicate that Cyp2b9 and 4a12 were present in a macrophage cell line, RAW264.7, and the regulation of P450 by inducers and cytokine differed from that in liver [8].
  • Monoclonal antibody Rip specifically recognizes 2',3'-cyclic nucleotide 3'-phosphodiesterase in oligodendrocytes [11].
  • In comparison with controls, isoniazid pretreatment of rats significantly increased the sp. act. of acetanilide 4-hydroxylase and the in vitro [14C]acetylhydrazine-derived covalent binding to hepatic microsomes but significantly decreased the sp. act. of benzo[a]pyrene hydroxylase and testosterone 16 alpha-hydroxylase [12].
  • The antigen recognized with monoclonal antibody (mAb) Rip (Rip-antigen) has been long used as a marker of oligodendrocytes and myelin sheaths [11].
 

Associations of Cyp2b9 with chemical compounds

  • Phenobarbital increased Cyp2b10 expression more than did Cyp2b9 in both sexes [13].
  • Treatment with dexamethasone markedly induced Cyp2b10 expression dose dependently, but simultaneously suppressed Cyp2b9 in both sexes [13].
  • Firstly, measurements of intracellular Fura-2 fluorescence ratio in murine hepatocytes showed that long-term PB incubation (24 and 48 h) led to a significant increase of [Ca2+]i. Secondly, treatment with an intracellular Ca2+ chelator (BAPTA-AM) nearly completely abolished PB-induced Cyp2b9/10 expression [14].
  • Our results showed a negative role of the cAMP-dependent pathway, as treatment with cAMP-dependent protein kinase (PKA) activators (10 microM dibutyryl-cAMP and 50 microM forskolin) dramatically inhibited PB-induced Cyp2b9/10 mRNA accumulation, whereas PKA inhibitor potentiated the PB responsiveness of this gene [14].
  • Tamoxifen or cryproterone, having antiestrogenic or antiandrogenic effects, respectively, also induced Cyp2b-9 and Cyp2b-10 mRNA [15].
 

Other interactions of Cyp2b9

 

Analytical, diagnostic and therapeutic context of Cyp2b9

References

  1. Direct test of potential roles of EIIIA and EIIIB alternatively spliced segments of fibronectin in physiological and tumor angiogenesis. Astrof, S., Crowley, D., George, E.L., Fukuda, T., Sekiguchi, K., Hanahan, D., Hynes, R.O. Mol. Cell. Biol. (2004) [Pubmed]
  2. Antitumor effects in mice of low-dose (metronomic) cyclophosphamide administered continuously through the drinking water. Man, S., Bocci, G., Francia, G., Green, S.K., Jothy, S., Hanahan, D., Bohlen, P., Hicklin, D.J., Bergers, G., Kerbel, R.S. Cancer Res. (2002) [Pubmed]
  3. Mouse liver testosterone 16 alpha-hydroxylase (cytochrome P-450(16) alpha). Purification, regioselectivity, stereospecificity, and immunochemical characterization. Harada, N., Negishi, M. J. Biol. Chem. (1984) [Pubmed]
  4. Genetic regulation of estrogen-dependent repression of female-specific testosterone 16 alpha-hydroxylase (I-P-450(16 alpha) in male mouse liver: murine Ripr locus. Noshiro, M., Negishi, M. Biochemistry (1988) [Pubmed]
  5. Rip locus: regulation of female-specific isozyme (I-P-450(16 alpha) of testosterone 16 alpha-hydroxylase in mouse liver, chromosome localization, and cloning of P-450 cDNA. Noshiro, M., Lakso, M., Kawajiri, K., Negishi, M. Biochemistry (1988) [Pubmed]
  6. Characterization of testosterone 16 alpha-hydroxylase (I-P-450(16) alpha) induced by phenobarbital in mice. Devore, K., Harada, N., Negishi, M. Biochemistry (1985) [Pubmed]
  7. Suppression of female-specific murine Cyp2b9 gene expression by growth or glucocorticoid hormones. Sakuma, T., Kitajima, K., Nishiyama, M., Mashino, M., Hashita, T., Nemoto, N. Biochem. Biophys. Res. Commun. (2004) [Pubmed]
  8. P450 isoforms in a murine macrophage cell line, RAW264.7, and changes in the levels of P450 isoforms by treatment of cells with lipopolysaccharide and interferon-gamma. Nakamura, M., Imaoka, S., Amano, F., Funae, Y. Biochim. Biophys. Acta (1998) [Pubmed]
  9. Transcriptional analysis in vivo of the hepatic genes, Cyp2b9 and Cyp2b10, by intravenous administration of plasmid DNA in mice. Rivera-Rivera, I., Kim, J., Kemper, B. Biochim. Biophys. Acta (2003) [Pubmed]
  10. Sex-associated expression of mouse hepatic and renal CYP2B enzymes by glucocorticoid hormones. Jarukamjorn, K., Sakuma, T., Yamamoto, M., Ohara, A., Nemoto, N. Biochem. Pharmacol. (2001) [Pubmed]
  11. Monoclonal antibody Rip specifically recognizes 2',3'-cyclic nucleotide 3'-phosphodiesterase in oligodendrocytes. Watanabe, M., Sakurai, Y., Ichinose, T., Aikawa, Y., Kotani, M., Itoh, K. J. Neurosci. Res. (2006) [Pubmed]
  12. Effect of isoniazid administration on selected rat and mouse hepatic microsomal mixed-function oxidases and in vitro [14C]acetylhydrazine-derived covalent binding. Powell-Jackson, P.R., Tredger, J.M., Smith, H.M., Davis, M., Williams, R. Biochem. Pharmacol. (1982) [Pubmed]
  13. Different regulation of the expression of mouse hepatic cytochrome P450 2B enzymes by glucocorticoid and phenobarbital. Jarukamjorn, K., Sakuma, T., Miyaura, J., Nemoto, N. Arch. Biochem. Biophys. (1999) [Pubmed]
  14. Regulation of phenobarbital induction of the cytochrome P450 2b9/10 genes in primary mouse hepatocyte culture. Involvement of calcium- and cAMP-dependent pathways. Marc, N., Galisteo, M., Lagadic-Gossmann, D., Fautrel, A., Joannard, F., Guillouzo, A., Corcos, L. Eur. J. Biochem. (2000) [Pubmed]
  15. Glucocorticoid and sex hormones as activating or modulating factors for expression of Cyp2b-9 and Cyp2b-10 in the mouse liver and hepatocytes. Nemoto, N., Sakurai, J. Arch. Biochem. Biophys. (1995) [Pubmed]
  16. Estrogen-dependent regulation of the expression of hepatic Cyp2b and 3a isoforms: assessment using aromatase-deficient mice. Yamada, H., Gohyama, N., Honda, S., Hara, T., Harada, N., Oguri, K. Toxicol. Appl. Pharmacol. (2002) [Pubmed]
  17. Maintenance of phenobarbital-inducible Cyp2b gene expression in C57BL/6 mouse hepatocytes in primary culture as spheroids. Nemoto, N., Sakurai, J., Funae, Y. Arch. Biochem. Biophys. (1995) [Pubmed]
  18. Structures and characterization of sex-specific mouse cytochrome P-450 genes as members within a large family. Duplication boundary and evolution. Lakso, M., Masaki, R., Noshiro, M., Negishi, M. Eur. J. Biochem. (1991) [Pubmed]
 
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