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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 
Gene Review: 

CYP2A7  -  cytochrome P450, family 2, subfamily A,...

Homo sapiens

Synonyms: CPA7, CPAD, CYP2A, CYPIIA7, Cytochrome P450 2A7, ...
 
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High impact information on CYP2A7

  • To explore whether the antiviral cellular immunity may turn against self-antigens, we characterized the primary CTL response against an HLA-A*0201-restricted HCV-derived epitope, i.e., HCV core 178-187, which shows sequence homology with human CYP2A6 and CYP2A7 8-17 [1].
  • Sequence differences in the CYP2A6v2 gene are restricted to regions encompassing exons 3, 6, and 8, which bear sequence relatedness with the corresponding exons of the CYP2A7 gene, located downstream and centromeric of CYP2A6v2, suggesting recent gene-conversion events [2].
  • A clone isolated from the liver cDNA library possessed 58 bp sequences in the 3'-untranslated region, which was replaced with the corresponding region of the CYP2A7 gene [3].
  • Interestingly, CYP2A7AS mRNA was the major CYP2A7 mRNA detected in the fibroblast cell line [4].
  • The relative level of CYP2A7 and CYP2A7AS mRNA was investigated by reverse transcription followed by PCR (RT-PCR) using human liver RNAs and an RNA sample from a human skin fibroblast cell line [4].
 

Biological context of CYP2A7

  • The results imply the presence of numerous defective as well as active CYP2A6 alleles as a consequence of CYP2A6/CYP2A7 gene conversion events [5].
  • Finally, haplotype analysis was carried out comprising the -745A > G variant in combination with all known CYP2A6 3' and 5' flanking single nucleotide polymorphisms: -1013A > G, -48T > G, and the CYP2A6/CYP2A7 3' flank conversion [6].
  • Two SNPs differentiating the 3'-UTR between CYP2A7 and CYP2A6*1B were found to be of importance for the expression in both systems [7].
  • In both systems, the 3'-UTR CYP2A6*1B constructs caused higher reporter gene activity and the CYP2A7 3'-UTR construct lower activity, compared to the CYP2A6*1 3'-UTR constructs [7].
  • We here focused on the polymorphism in the 3'-UTR region, in particular the common CYP2A6*1B allele, carrying an unequal crossover element from the pseudogene CYP2A7 [7].
 

Anatomical context of CYP2A7

  • The expression of these cDNAs in COS-7 cells showed that both CYP2A6 and CYP2A7 generated a protein of molecular mass 49 kDa, whereas the protein product of CYP2A7AS was about 44 kDa [4].
 

Associations of CYP2A7 with chemical compounds

  • CYP2A6 is active toward many carcinogens and is the major coumarin 7-hydroxylase and nicotine C-oxidase in the liver, whereas CYP2A7 is not functional [8].
  • MAb CPA7 or CPA9 was coupled to CNBr activated sepharose and affinity purification of the major allergen of CPAg from the crude extract was performed [9].
 

Other interactions of CYP2A7

  • A variant CYP2A6 allele that differed from the corresponding CYP2A6 and CYP2A7 cDNAs previously sequenced was found and was designated CYP2A6v2 [2].
 

Analytical, diagnostic and therapeutic context of CYP2A7

  • PCR analysis using primers specific to the CYP2A7 gene and the CYP2A6 and CYP2A7 genes confirmed that all DNA samples obtained from 7 individuals carrying the E-type variant possessed the same break points [10].

References

  1. Molecular mimicry of human cytochrome P450 by hepatitis C virus at the level of cytotoxic T cell recognition. Kammer, A.R., van der Burg, S.H., Grabscheid, B., Hunziker, I.P., Kwappenberg, K.M., Reichen, J., Melief, C.J., Cerny, A. J. Exp. Med. (1999)
  2. A genetic polymorphism in coumarin 7-hydroxylation: sequence of the human CYP2A genes and identification of variant CYP2A6 alleles. Fernandez-Salguero, P., Hoffman, S.M., Cholerton, S., Mohrenweiser, H., Raunio, H., Rautio, A., Pelkonen, O., Huang, J.D., Evans, W.E., Idle, J.R. Am. J. Hum. Genet. (1995)
  3. Structural characterization of a new variant of the CYP2A6 gene (CYP2A6*1B) apparently diagnosed as heterozygotes of CYP2A6*1A and CYP2A6*4C. Ariyoshi, N., Takahashi, Y., Miyamoto, M., Umetsu, Y., Daigo, S., Tateishi, T., Kobayashi, S., Mizorogi, Y., Loriot, M.A., Stücker, I., Beaune, P., Kinoshita, M., Kamataki, T. Pharmacogenetics (2000)
  4. Expression and alternative splicing of the cytochrome P-450 CYP2A7. Ding, S., Lake, B.G., Friedberg, T., Wolf, C.R. Biochem. J. (1995)
  5. Identification and characterisation of novel polymorphisms in the CYP2A locus: implications for nicotine metabolism. Oscarson, M., McLellan, R.A., Gullstén, H., Agúndez, J.A., Benítez, J., Rautio, A., Raunio, H., Pelkonen, O., Ingelman-Sundberg, M. FEBS Lett. (1999)
  6. Polymorphic NF-Y dependent regulation of human nicotine C-oxidase (CYP2A6). von Richter, O., Pitarque, M., Rodríguez-Antona, C., Testa, A., Mantovani, R., Oscarson, M., Ingelman-Sundberg, M. Pharmacogenetics (2004)
  7. 3'-UTR polymorphism in the human CYP2A6 gene affects mRNA stability and enzyme expression. Wang, J., Pitarque, M., Ingelman-Sundberg, M. Biochem. Biophys. Res. Commun. (2006)
  8. Human cytochrome P450 CYP2A13: predominant expression in the respiratory tract and its high efficiency metabolic activation of a tobacco-specific carcinogen, 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone. Su, T., Bao, Z., Zhang, Q.Y., Smith, T.J., Hong, J.Y., Ding, X. Cancer Res. (2000)
  9. Production of monoclonal antibodies specific to major allergens of Cryptomeria japonica pollen. Honda, K., Shirae, H., Miwa, K., Matsushita, S., Makishima, K., Nishimura, Y., Sasazuki, T. Hybridoma (1992)
  10. Homologous unequal cross-over within the human CYP2A gene cluster as a mechanism for the deletion of the entire CYP2A6 gene associated with the poor metabolizer phenotype. Nunoya, K., Yokoi, T., Takahashi, Y., Kimura, K., Kinoshita, M., Kamataki, T. J. Biochem. (1999)
 

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