Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

Javascript disabled

Please enable Javascript support in your browser to use this application.

Instructions on how to enable JavaScript on different browsers can be found here: http://www.google.com/support/bin/answer.py?answer=23852.

Gene: EEF1D - eukaryotic translation elongation factor 1...

Homo sapiens

Synonyms: Antigen NY-CO-4, EF1D, EF-1D, EF-1-delta, Elongation factor 1-delta, FLJ20897, FP1047

[edit this page]

Kolettas, E., Ong, L.L., Bec, G., Delalande, C., De Bortoli, M., et al.

Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text.

Ideally this entry shall become one comprehensive and continuous article. Bulleted lists, for instance, were only used because it is impossible to automatically integrate independent facts into a continuous text.

Much of the current information on this page has been automatically compiled from Pubmed.

This precompiled information serves as a substrate and matrix to embed your contributions, but it is by no means the final word - Homo sapiens can do much better!

WikiGenes is a non-profit and open access community project.

Disease relevance of EEF1D

We demonstrate that gain of 8q and expression levels of three 8q-mapped candidate genes (EEF1D, RPL30, RPS20) are associated with adverse outcome in medulloblastoma [1].
Here we show that four proteins, namely the translationally controlled tumor protein, the human elongation factor 1-delta, tetratricopeptide repeat protein and the isoform 14-3-3-gamma of the 14-3-3-family are overexpressed in chemoresistant melanoma cell lines [2].
A higher expression of EF-1 delta was significantly correlated with lymph node metastases (P<0.05) and advanced stages (P<0.05) [3].
Here, we report that the human translation elongation factor 1-delta (EF-1 delta) binds to the second exon of HIV-1 Tat [4].

High impact information on EEF1D

Cell cycle analysis shows that EF-1 delta expression enhancement precedes G2 arrest in these cells, suggesting that EF-1 delta may participate in the G2-M cell cycle checkpoint [5].
The minimal EF-1 delta-binding domain on kinectin resides within a conserved region present in all the kinectin isoforms [6].
EF-1 delta provides the guanine nucleotide exchange activities on EF-1 alpha during elongation step of protein synthesis [6].
Kinectin anchors the translation elongation factor-1 delta to the endoplasmic reticulum [6].
The results demonstrate that the NH2-terminal extension of valyl-tRNA synthetase is required for complex formation and that the enzyme-binding site(s) resides on the EF-1 delta subunit [7].

Biological context of EEF1D

Medulloblastoma outcome is adversely associated with overexpression of EEF1D, RPL30, and RPS20 on the long arm of chromosome 8 [1].
Phosphorylation studies revealed the presence of two post-translational regulated forms of EF-1 delta protein [8].
To explore the relation between oncogenes and EF-1 delta expression, a variety of oncogenes were introduced into human mammary epithelial cells (MCF-7) and human keratinocytes (HaCaT) [9].
The higher expression of EF-1 delta in the tumours suggested that malignant transformation in vivo requires an increase in translation factor mRNA and protein synthesis for entry into and transition through the cell cycle [9].
To explore the relation between cell division and EF-1 delta expression, MCF-7 cells were treated with dexamethasone, an inducer of differentiation [9].

Anatomical context of EEF1D

Elongation factor EF-1 delta, a new target for maturation-promoting factor in Xenopus oocytes [8].
EF-1 delta mRNA is present as a single 2.7-kb transcript in unfertilized eggs and in rapidly dividing cleavage stage embryos [10].
Since the 2.0-kb polyadenylated form of the EF-1 delta transcript appears at gastrula stage, our results suggest that a mechanism for alternative poly(A) site selection of the EF-1 delta transcript appears during embryonic development [10].
Four of the identified proteins were differentially phosphorylated: annexin-1, an actin-binding protein and a substrate for receptor tyrosine kinases; tropomyosin-alpha, a regulator of actin filament stability and cell shape; elongation factor 1 delta; ATP synthase beta-chain [11].

Associations of EEF1D with chemical compounds

EF-1 delta is phosphorylated by Cdc2 kinase on threonine and serine residues [8].
There was no change in the mRNA levels of EF-1 delta in the dexamethasone-treated cells [9].

Other interactions of EEF1D

Reconstitution in vitro of the valyl-tRNA synthetase-elongation factor (EF) 1 beta gamma delta complex. Essential roles of the NH2-terminal extension of valyl-tRNA synthetase and of the EF-1 delta subunit in complex formation [7].
Identification of the interaction between the human recombinant histamine releasing factor/translationally controlled tumor protein and elongation factor-1 delta (also known as eElongation factor-1B beta) [12].
This report provides evidence of an alternative kinectin function as the membrane anchor for EF-1 delta on the endoplasmic reticulum and provides clues to the EF-1 complex assembly and anchorage on the endoplasmic reticulum [6].
In this report, we demonstrate that three components of EF-1H (EF-1 beta, EF-1 delta, EF-1 gamma) bind to DNA modified with chromium (Cr), a potent DNA-damaging agent and an established human carcinogen [13].
Despite high oncogene mRNA expression, there was no significant change in the EF-1 delta mRNA level by v-src, c-erbB (EGF Receptor), c-erbB-2, v-myc and v-fos oncogenes [9].

References

Medulloblastoma outcome is adversely associated with overexpression of EEF1D, RPL30, and RPS20 on the long arm of chromosome 8. De Bortoli, M., Castellino, R.C., Lu, X.Y., Deyo, J., Sturla, L.M., Adesina, A.M., Perlaky, L., Pomeroy, S.L., Lau, C.C., Man, T.K., Rao, P.H., Kim, J.Y. BMC Cancer (2006)
Identification of novel proteins associated with the development of chemoresistance in malignant melanoma using two-dimensional electrophoresis. Sinha, P., Kohl, S., Fischer, J., Hütter, G., Kern, M., Köttgen, E., Dietel, M., Lage, H., Schnölzer, M., Schadendorf, D. Electrophoresis (2000)
Clinical significance of elongation factor-1 delta mRNA expression in oesophageal carcinoma. Ogawa, K., Utsunomiya, T., Mimori, K., Tanaka, Y., Tanaka, F., Inoue, H., Murayama, S., Mori, M. Br. J. Cancer (2004)
Interaction of the second coding exon of Tat with human EF-1 delta delineates a mechanism for HIV-1-mediated shut-off of host mRNA translation. Xiao, H., Neuveut, C., Benkirane, M., Jeang, K.T. Biochem. Biophys. Res. Commun. (1998)
Elongation factor 1 delta is enhanced following exposure to ionizing radiation. Jung, M., Kondratyev, A.D., Dritschilo, A. Cancer Res. (1994)
Kinectin anchors the translation elongation factor-1 delta to the endoplasmic reticulum. Ong, L.L., Er, C.P., Ho, A., Aung, M.T., Yu, H. J. Biol. Chem. (2003)
Reconstitution in vitro of the valyl-tRNA synthetase-elongation factor (EF) 1 beta gamma delta complex. Essential roles of the NH2-terminal extension of valyl-tRNA synthetase and of the EF-1 delta subunit in complex formation. Bec, G., Kerjan, P., Waller, J.P. J. Biol. Chem. (1994)
Elongation factor EF-1 delta, a new target for maturation-promoting factor in Xenopus oocytes. Mulner-Lorillon, O., Minella, O., Cormier, P., Capony, J.P., Cavadore, J.C., Morales, J., Poulhe, R., Bellé, R. J. Biol. Chem. (1994)
Modulation of elongation factor-1 delta (EF-1 delta) expression by oncogenes in human epithelial cells. Kolettas, E., Lymboura, M., Khazaie, K., Luqmani, Y. Anticancer Res. (1998)
Developmental regulation of elongation factor-1 delta in sea urchin suggests appearance of a mechanism for alternative poly(A) site selection in gastrulae. Delalande, C., Monnier, A., Minella, O., Genevière, A.M., Mulner-Lorillon, O., Bellé, R., Cormier, P. Exp. Cell Res. (1998)
Proteome analysis of human mesothelial cells during epithelial to mesenchymal transitions induced by shed menstrual effluent. Demir, A.Y., Demol, H., Puype, M., de Goeij, A.F., Dunselman, G.A., Herrler, A., Evers, J.L., Vandekerckhove, J., Groothuis, P.G. Proteomics (2004)
Identification of the interaction between the human recombinant histamine releasing factor/translationally controlled tumor protein and elongation factor-1 delta (also known as eElongation factor-1B beta). Langdon, J.M., Vonakis, B.M., MacDonald, S.M. Biochim. Biophys. Acta (2004)
DNA binding activity of the mammalian translation elongation complex: recognition of chromium- and transplatin-damaged DNA. Wang, J.F., Engelsberg, B.N., Johnson, S.W., Witmer, C., Merrick, W.C., Rozmiarek, H., Billings, P.C. Arch. Toxicol. (1997)