Disease relevance of car1

• Amiloride toxicity in the fission yeast Schizosaccharomyces pombe is released by thiamine and mutations in the thiamine-repressible gene car1 [1].

High impact information on car1

• It seems that immediate arginase degradation is initiated by starved cells or ones from which arginine has been removed [2].
• Induction of arginase and ornithine transaminase in the fission yeast Schizosaccharomyces pombe [2].
• Amiloride-resistant mutants were isolated and shown to represent a new locus (car1 for changed amiloride resistance) on chromosome I. The car1 gene was cloned and sequenced [3].
• We propose that car1 serves an uptake function, perhaps as a symport with an unknown substrate and this carrier may transport amiloride into the cell [3].
• Gene disruption of car1 results in increased amiloride resistance. car1 has sequence similarity to proteins from Candida associated with resistance to benomyl, methotrexate and cycloheximide [3].

References