Disease relevance of Cetn1

• In the studies reported here, recombinant Chlamydomonas caltractin was expressed at high levels in Escherichia coli and purified to homogeneity [1].

High impact information on Cetn1

• All approaches led to reduced targeting of centrin, pericentrin, and ninein to the centrosome [2].
• Calcium-dependent assembly of centrin-G-protein complex in photoreceptor cells [3].
• Here we demonstrate that transducin colocalizes with the Ca(2+)-binding protein centrin 1 in a specific domain of this cilium [3].
• Spindle poles of unfertilized eggs and blastocysts were found to react positively with two antisera (centrin and NRS-01), whereas poles of activated eggs and early cleavage-stage embryos were negative when treated with the same sera [4].
• Unlike ciliated olfactory neurons, they express neither olfactory marker protein nor centrin, adenylyl cyclase or cyclic nucleotide-gated cation channels [5].

Biological context of Cetn1

• The Ca(2+)-dependent assembly of a G protein with centrin is a novel aspect of the supply of signaling proteins in sensory cells and a potential link between molecular translocations and signal transduction in general [3].
• Centriole and centrin degeneration during mouse spermiogenesis [6].

Anatomical context of Cetn1

• RT-PCR shows that Cetn2 and Cetn3, but not Cetn1, are expressed in NIH 3T3 cells [7].
• TEM studies after immunogold labeling revealed that centrin is associated with the distal and the proximal centrioles, but not with the adjunct [6].
• Centrin is detected as two spots in round spermatids, corresponding to a pair of centrioles [6].
• In elongating spermatids, centrin spots colocalize with the centrioles in the neck region, while the perinuclear ring from which manchette microtubules arise, does not label with the antibody 20H5 [6].

Associations of Cetn1 with chemical compounds

• Centrosomes were isolated from detergent lysates of FDCP cells by sucrose density gradient centrifugation and contain tubulin (M(r) = 58 kDa) and centrin (M(r) = 20 kDa) by immunoblotting [8].

Other interactions of Cetn1

• Cetn2 encodes a 172 amino acid protein, with a predicted molecular mass of 19,795 Da (pI=4.71), that contains all of the defining characteristics of centrin [7].
• The assembly of centrin-G-protein complex is mediated by the betagamma-complex [3].
• The striated bands of Paramecium are immunologically distinct from the centrin-specific infraciliary lattice and cytostomal cord [9].
• Double-label immunofluorescence with antibodies against gamma-tubulin, detyrosinated alpha-tubulin, and centrin showed that the TU-06 epitope is localized outside the basal body region [10].

Analytical, diagnostic and therapeutic context of Cetn1

• Coimmunoprecipitation, centrifugation, centrin overlay, size exclusion chromatography, and kinetic light-scattering experiments indicate that Ca(2+)-activated centrin 1 binds with high affinity and specificity to transducin [3].
• Among the numerous centrin isoforms identified by two-dimensional gel electrophoresis in human cells, an acidic and slow-migrating isoform is particularly enriched in a centrosome fraction [11].

References