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RGN  -  regucalcin

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Disease relevance of RGN

  • The hepatoma cells (wild-type) and stable regucalcin/pCXN2-transfected cells (transfectant) were cultured for 72 h in a medium containing 10% fetal bovine serum (FBS) to obtain subconfluent monolayers [1].
 

High impact information on RGN

  • Overexpression of regucalcin suppresses cell response for tumor necrosis factor-alpha or transforming growth factor-beta1 in cloned normal rat kidney proximal tubular epithelial NRK52E cells [2].
  • Overexpression of regucalcin enhances glucose utilization and lipid production in cloned rat hepatoma H4-II-E cells: Involvement of insulin resistance [1].
  • The effect of regucalcin (RC), a regulatory protein in intracellular signaling pathway, on the gene expression of various mineral ion transport-related proteins was investigated using the cloned normal rat kidney proximal tubular epithelial NRK52E cells overexpressing RC [3].
  • Overexpression of RGPR-p117 enhances regucalcin gene promoter activity in cloned normal rat kidney proximal tubular epithelial cells: Involvement of TTGGC motif [4].
  • This study demonstrates that RGPR-p117 can enhance the regucalcin promoter activity which is related to the NF-1 consensus sequences including TTGGC motif, and that its enhancing effect is partly mediated through phosphorylation and dephosphorylation in NRK52E cells [4].
 

Chemical compound and disease context of RGN

  • The present study suggests that the regucalcin mRNA expression is mediated through signaling pathways which are partly involved in Ca2+-dependent protein kinases and tyrosine kinase in H4-II-E hepatoma cells [5].
  • This study demonstrates that overexpression of regucalcin enhances glucose utilization and lipid production in the cloned rat hepatoma H4-II-E cells, and that it regulates the effect of insulin [1].
  • Overexpression of regucalcin suppresses cell death and apoptosis in cloned rat hepatoma H4-II-E cells induced by lipopolysaccharide, PD 98059, dibucaine, or Bay K 8644 [6].
 

Biological context of RGN

  • Comparison analysis revealed that the nucleotide sequences of regucalcin from seven vertebrate species were highly conserved in their coding region [7].
  • This study demonstrates that overexpression of regucalcin has a suppressive effect on cell death induced by LPS or various intracellular signaling-related factors [6].
  • This study demonstrates that cell proliferation is suppressed in the cloned rat hepatoma H4-II-E overexpressing RC stably [8].
  • This study demonstrates that overexpression of RC caused a remarkable increase in its nuclear localization, and that it has suppressive effects on the gene expression of L-type Ca(2+) channel or CaR, which regulates intracellular Ca(2+) signaling, among various regulator proteins for mineral ions in NRK52E cells [3].
  • The change in regucalcin mRNA levels was analyzed by Northern blotting using rat liver regucalcin complementary DNA (0.9 kb of open reading frame) [5].
 

Anatomical context of RGN

  • Regucalcin is a Ca2+-binding protein, which plays a regulatory role in liver cell functions related to Ca2+ [7].
  • A calcium-binding protein, regucalcin, was isolated from rat liver cytosol [9].
 

Associations of RGN with chemical compounds

  • Meanwhile, the regucalcin mRNA expression was significantly stimulated by the addition of Bay K 8644 (2.5 x 10(-6) M) in the presence of serum [5].
  • H4-II-E cells were transfected with RC/pCXN2 vector and the multiple neomycin-resistant clones which overexpress stably RC were selected [8].
  • The effect of regucalcin in decreasing cellular protein content was significantly inhibited in the presence of various kinase inhibitors including staurosporine (10(-7) M), dibucaine (10(-6) M), PD98059 (10(-8) M), or wortmannin (10(-8) M) [10].
  • Rabbit-anti-regucalcin antiserum, which was raised against regucalcin conjugated by glutaraldehyde to bovine serum albumin, was applied to glutaraldehyde-fixed whole mounts and subsequently visualized using the peroxidase-antiperoxidase methods [9].
  • Moreover, the effect of antibody in increasing protein kinase activity was significantly inhibited in the presence of trifluoperazine, staurosporine, or genistein, indicating that endogenous regucalcin has an inhibitory effect on Ca(2+)/calmodulin-dependent protein kinase, protein kinase C, and protein tyrosine kinase [11].
 

Analytical, diagnostic and therapeutic context of RGN

  • The molecular cloning and sequencing of the cDNA coding for a novel regucalcin gene promoter region-related protein (RGPR-p117) from bovine, rabbit and chicken livers was investigated using rapid amplification of cDNA endo (RACE) method [12].

References

  1. Overexpression of regucalcin enhances glucose utilization and lipid production in cloned rat hepatoma H4-II-E cells: Involvement of insulin resistance. Nakashima, C., Yamaguchi, M. J. Cell. Biochem. (2006) [Pubmed]
  2. Overexpression of regucalcin suppresses cell response for tumor necrosis factor-alpha or transforming growth factor-beta1 in cloned normal rat kidney proximal tubular epithelial NRK52E cells. Nakagawa, T., Yamaguchi, M. J. Cell. Biochem. (2007) [Pubmed]
  3. Overexpression of regucalcin enhances its nuclear localization and suppresses L-type Ca(2+) channel and calcium-sensing receptor mRNA expressions in cloned normal rat kidney proximal tubular epithelial NRK52E cells. Nakagawa, T., Yamaguchi, M. J. Cell. Biochem. (2006) [Pubmed]
  4. Overexpression of RGPR-p117 enhances regucalcin gene promoter activity in cloned normal rat kidney proximal tubular epithelial cells: Involvement of TTGGC motif. Sawada, N., Yamaguchi, M. J. Cell. Biochem. (2006) [Pubmed]
  5. Involvement of intracellular signaling factors in the serum-enhanced Ca2+-binding protein regucalcin mRNA expression in the cloned rat hepatoma cells (H4-II-E). Yamaguchi, M., Nakajima, M. J. Cell. Biochem. (1999) [Pubmed]
  6. Overexpression of regucalcin suppresses cell death and apoptosis in cloned rat hepatoma H4-II-E cells induced by lipopolysaccharide, PD 98059, dibucaine, or Bay K 8644. Izumi, T., Yamaguchi, M. J. Cell. Biochem. (2004) [Pubmed]
  7. The gene of Ca2+-binding protein regucalcin is highly conserved in vertebrate species. Misawa, H., Yamaguchi, M. Int. J. Mol. Med. (2000) [Pubmed]
  8. Suppression of cell proliferation and deoxyribonucleic acid synthesis in the cloned rat hepatoma H4-II-E cells overexpressing regucalcin. Misawa, H., Inagaki, S., Yamaguchi, M. J. Cell. Biochem. (2001) [Pubmed]
  9. Immunohistochemical demonstration of calcium-binding protein regucalcin in the tissues of rats: the protein localizes in liver and brain. Yamaguchi, M., Isogai, M., Kato, S., Mori, S. Chem. Pharm. Bull. (1991) [Pubmed]
  10. Regulatory effect of exogenous regucalcin on cell function in osteoblastic MC3T3-E1 cells: involvement of intracellular signaling factor. Otomo, Y., Yamaguchi, M. Int. J. Mol. Med. (2006) [Pubmed]
  11. Suppressive role of endogenous regucalcin in the enhancement of protein kinase activity with proliferation of cloned rat hepatoma cells (H4-II-E). Inagaki, S., Yamaguchi, M. J. Cell. Biochem. Suppl. (2001) [Pubmed]
  12. A novel regucalcin gene promoter region-related protein: comparison of nucleotide and amino acid sequences in vertebrate species. Sawada, N., Yamaguchi, M. Int. J. Mol. Med. (2005) [Pubmed]
 
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