GULO - L-gulono-gamma-lactone oxidase
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High impact information on GULO
- Enhancement of NO production by intracellular AA was further verified in aortic endothelial cells obtained from eNOS knockout mice that were cotransfected with eNOS and GULO constructs .
- GULO-dependent AA synthesis also elevated intracellular tetrahydrobiopterin content, implicating that this essential cofactor of endothelial nitric oxide synthase (eNOS) might mediate the AA effect .
- To restore AA synthesis pathway, bovine aortic endothelial cells were transfected with the plasmid vector encoding L-gulono-1,4-lactone oxidase (GULO, EC 18.104.22.168), the missing enzyme converting L-gulono-1,4-lactone (GUL) to AA .
- When the cells were stimulated with calcium ionophore, A23187, NO production was more active in the GULO-expressing cells supplied with GUL, in comparison with the cells without GULO expression or without GUL supply, indicating that intracellular AA regulated NO production .
Analytical, diagnostic and therapeutic context of GULO
- Functional expression of GULO was verified by Western blotting and in vitro enzyme activity assay .
- Ascorbic acid synthesis due to L-gulono-1,4-lactone oxidase expression enhances NO production in endothelial cells. Kim, H.J., Lee, S.I., Lee, D.H., Smith, D., Jo, H., Schellhorn, H.E., Boo, Y.C. Biochem. Biophys. Res. Commun. (2006) [Pubmed]