Disease relevance of Plcg2

• Using an Escherichia coli expression system, we succeeded in producing active PLC IV in E. coli crude extracts [1].

High impact information on Plcg2

• In these reconstituted cells, Fc epsilon RI aggregation induced tyrosine phosphorylation of phospholipase C-gamma 1 and phospholipase C-gamma 2, an increase in intracellular free Ca2+ and histamine release [2].
• In addition, incubation of RBL cells with either oligomeric IgE or orthovanadate results in the translocation of PLC-gamma 2 from the cytosol to the particulate fraction [3].
• Isolation and characterization of rat 3Y1 fibroblast clones overexpressing the src homology region of phospholipase C-gamma 2 [4].
• A fourth type of rat phosphoinositide-specific phospholipase C (PLC IV) has been cloned for cDNA and sequenced [1].
• PLC IV is distinct from the other three types of rat PLC (PLC I, II, and III) with respect to primary structure and tissue distribution of its mRNAs [1].

Biological context of Plcg2

• PLC IV, in common with PLC II, has a sequence homologous to the N-terminal regulatory domains of nonreceptor tyrosine kinases of the src-family of oncogenes [1].
• The substrate specificity of phosphoinositide-phospholipase C in rat heart sarcolemma [5].

Anatomical context of Plcg2

• Translocation of phospholipase C-gamma 2 induced by in vitro activation of protein tyrosine kinase activity in mast cell lysates [6].
• PLC gamma 2 mRNA was localized only in the Purkinje and granule cells in the vermal portions of lobules IX and X of the cerebellum, and in the adenohypophysis [7].

Associations of Plcg2 with chemical compounds

• Both tyrosine phosphorylation and PLC gamma 2 translocation are inhibited by genistein [6].
• We determined the effect of purified rP45Z or rP38Z on PIP2-hydrolyzing activity of either PLC-gamma 1 or PLC-gamma 2 and found that these proteins strongly suppressed the rate of PLC-dependent PIP2-hydrolysis [8].

Other interactions of Plcg2

• On the other hand, PLC-beta 4, PLC-gamma 1 and PLC-gamma 2 were distributed almost equally among the regions for the rats of both ages [9].

References