Disease relevance of Hexa

• After 6 or 12 weeks of hypertension, specific activities of all enzymes measured were significantly increased, levels ranging from 24% above normal for cathepsin C to 351% above normal for N-acetyl-beta-glucosaminidase [1].
• When homogenates of rat liver and hepatomas were centrifuged at 78 000 X g, over 90% of liver N-acetylglucosaminyltransferase assayed with beta-galactosidase- and beta-N-acetylhexosaminidase-treated asialofetuin as acceptor was recovered in the particulate fraction, while as much as 24% of hepatoma transferase was in the supernatant fraction [2].

High impact information on Hexa

• Delivery of hexosaminidase A to the cerebrum after osmotic modification of the blood--brain barrier [3].
• Enzymes included: neutral alpha-glucosidase and lysosomal beta-galactosidase, N-acetyl-beta-glucosaminidase, cathepsin C, acid alpha-glucosidase, and acid cholesteryl esterase [4].
• In every instance, histochemical studies of aortas showed acid phosphatase and N-acetyl-beta-glucosaminidase activities which corresponded to the biochemical findings [1].
• These studies provide evidence that blood-brain barrier modification permits delivery of functionally active hexosaminidase A into subcellular organelles consistent with that known to be the appropriate site of physiologic activity [5].
• Hexa- and pentapeptide extension of proalbumin: feedback inhibition of albumin synthesis by its propeptide in isolated hepatocytes and in the cell-free system [6].

Biological context of Hexa

• The use of urinary N-acetyl-beta-glucosaminidase in human renal toxicology. I. Partial biochemical characterization and excretion in humans and release from the isolated perfused rat kidney [7].

Anatomical context of Hexa

• However, after incubation with 220 microM of estramustine, most drug equilibrated in heavier fractions with high concentrations of N-acetyl-beta-glucosaminidase and cathepsin B, marker enzymes for the lysosomes as well as NADPH:cytochrome c reductase, marker enzyme for the endoplasmic reticulum [8].
• Activity of N-acetyl-beta-glucosaminidase in lymphocytes of rats exposed to mixture of nitrogen oxides and chlorine [9].
• Fluorometric assays were performed for hexosaminidase A (HEX A) and beta-glucuronidase (GLUC), markers of enterocyte necrosis [10].
• Mast cell granule constituents such as histamine, heparin, mast cell protease and beta-glucuronidase increased steadily during the observation period, whereas the amount of N-acetyl-beta-glucosaminidase decreased during the first 2 weeks and was thereafter constant [11].

Associations of Hexa with chemical compounds

• The growth hormone-releasing peptide Hexarelin (Hexa; 80 micrograms/kg-1, s.c.) was administered for 30 and 60 days to old rats [12].
• It also increased the content of acidic glycosaminoglycans (AGAG), which are thought to be closely related to the formation of collagen fibers, and increased the activity of glutamine glucose-6-phosphate aminotransferase, an enzyme for AGAG synthesis but not that of N-acetyl-beta-glucosaminidase, an enzyme for AGAG degradation [13].
• At the beginning and after 12 weeks of the study renal creatinine clearance, urinary excretion of albumin, N-acetyl-beta-glucosaminidase (NAG) and cytokines were measured [14].
• Comments on the cytochemical techniques using naphthol AS-BI substrates and hexazonium pararosanilin for the demonstration of acid phosphatase, beta-glucuronidase and N-acetyl-beta-glucosaminidase activities [15].
• The penetration of methotrexate, Evans blue-albumin, and hexosaminidase A was then evaluated at intervals ranging from 1.5 to 3.5 hours after administration [16].

Other interactions of Hexa

• Somatostatin mRNA concentrations were significantly higher in the hypothalami of older rats and administration of Hexa for 30 or 60 days brought the concentrations of somatostatin mRNA of aged rats to 'young' levels [12].
• To analyze transcription levels of Hex alpha and beta subunits (Hexa and Hexb, respectively), real-time relative quantitative reverse transcription-polymerase chain reaction (RT-PCR) analysis was performed using the LightCycler system [17].

Analytical, diagnostic and therapeutic context of Hexa

• The acid phosphatase and beta-N-acetylhexosaminidase isoenzymes were separated by isoelectric focusing [18].
• A single intraperitoneal injection of bromobenzene (5 mmoles/kg) caused significant increases in the total activity of lysosomal N-acetyl-beta-glucosaminidase and beta-glucuronidase in rat liver within 8-12 h, whereas it did not alter the free activity of these enzymes [19].

References