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Gene Review

Rrp1  -  Recombination repair protein 1

Drosophila melanogaster

Synonyms: 3178, CG3178, DNA-(apurinic or apyrimidinic site) lyase, Dmel\CG3178, RRP1, ...
 
 
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Disease relevance of Rrp1

 

High impact information on Rrp1

  • These data suggest a lesion-specific involvement of Rrp1 in the repair of oxidative DNA damage [1].
  • A large decrease in mosaic clone frequency is observed when Rrp1 overexpression precedes treatment with gamma-rays, bleomycin, or paraquat [1].
  • By using the Drosophila w/w+ mosaic eye system, which detects loss of heterozygosity as changes in eye pigmentation, somatic mutation and recombination frequencies were determined in transgenic flies overexpressing wild-type Rrp1 protein from a heat-shock-inducible transgene [1].
  • In contrast, Rrp1 overexpression does not alter the spot frequency after treatment with the alkylating agents methyl methanesulfonate or methyl nitrosourea [1].
  • Interestingly, DmREV7 bound to the N-terminal region of Rrp1, which has no known protein homologue, suggesting that this binding is a species-specific event [2].
 

Biological context of Rrp1

 

Associations of Rrp1 with chemical compounds

  • Rrp1 exonuclease activity is examined at 3'-terminal homopurine or homopyrimidine tracts, at junctions between purine- and pyrimidine-rich sequences and upon encountering repeated dinucleotide runs [5].
  • Rrp1 cleaves the phosphoglycolate and releases a product with a 3'-hydroxyl terminus [7].
  • In that assay, Rrp1 poorly repairs 3'-phosphate-terminated nicks introduced by micrococcal nuclease [7].
 

Physical interactions of Rrp1

 

Other interactions of Rrp1

  • On the basis of the direct interaction between DmREV7 and Rrp1, we suggest that Dmpol zeta may be involved in the repair pathway of AP sites in DNA [2].
  • The Rrp1 cleavage product comigrates with a DNaseI cleavage product, and the newly formed terminus supports DNA synthesis by DNA polymerase [8].
  • A model for achiasmatic pairing in Drosophila males based upon the combined action of topoisomerase I and a strand transferase is proposed [9].

References

  1. Overexpression of a Rrp1 transgene reduces the somatic mutation and recombination frequency induced by oxidative DNA damage in Drosophila melanogaster. Szakmary, A., Huang, S.M., Chang, D.T., Beachy, P.A., Sander, M. Proc. Natl. Acad. Sci. U.S.A. (1996) [Pubmed]
  2. Drosophila DNA Polymerase {zeta} Interacts with Recombination Repair Protein 1, the Drosophila Homologue of Human Abasic Endonuclease 1. Takeuchi, R., Ruike, T., Nakamura, R., Shimanouchi, K., Kanai, Y., Abe, Y., Ihara, A., Sakaguchi, K. J. Biol. Chem. (2006) [Pubmed]
  3. Cloning and expression of APE, the cDNA encoding the major human apurinic endonuclease: definition of a family of DNA repair enzymes. Demple, B., Herman, T., Chen, D.S. Proc. Natl. Acad. Sci. U.S.A. (1991) [Pubmed]
  4. Single amino acid changes alter the repair specificity of Drosophila Rrp1. Isolation of mutants deficient in repair of oxidative DNA damage. Gu, L., Huang, S.M., Sander, M. J. Biol. Chem. (1994) [Pubmed]
  5. Drosophila Rrp1 3'-exonuclease: demonstration of DNA sequence dependence and DNA strand specificity. Sander, M., Benhaim, D. Nucleic Acids Res. (1996) [Pubmed]
  6. Drosophila melanogaster strand transferase. A protein that forms heteroduplex DNA in the absence of both ATP and single-strand DNA binding protein. Lowenhaupt, K., Sander, M., Hauser, C., Rich, A. J. Biol. Chem. (1989) [Pubmed]
  7. Characterization of the nuclease activity of Drosophila Rrp1 on phosphoglycolate- and phosphate-modified DNA 3'-termini. Sander, M., Huang, S.M. Biochemistry (1995) [Pubmed]
  8. Characterization of the apurinic endonuclease activity of Drosophila Rrp1. Nugent, M., Huang, S.M., Sander, M. Biochemistry (1993) [Pubmed]
  9. Evidence that intergenic spacer repeats of Drosophila melanogaster rRNA genes function as X-Y pairing sites in male meiosis, and a general model for achiasmatic pairing. McKee, B.D., Habera, L., Vrana, J.A. Genetics (1992) [Pubmed]
 
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