High impact information on GNAT3

• We also demonstrate that the activation in response to denatonium of gustducin by presumptive bitter-responsive receptors present in taste membranes depends on an interaction with the C terminus of gustducin and requires G-protein betagamma subunits to provide the receptor-interacting heterotrimer [1].
• In this paper, we show that gustducin is expressed in bovine taste tissue and that both gustducin and transducin, in the presence of bovine taste membranes, can be activated specifically by several bitter compounds, including denatonium, quinine, and strychnine [1].
• G gamma(13) is a divergent member of the G gamma subunit family considered to be a component of the gustducin G-protein heterotrimer involved in bitter and sweet taste reception in taste bud cells [2].
• Colocalization of the {alpha}-subunit of gustducin with PYY and GLP-1 in L cells of human colon [3].
• Next, we examined the correlation of gene expression of the candidate components leading to PLCbeta2 activation in rat circumvallate papillae, including G proteins, G(i2) and gustducin, and a G protein-coupled receptor, TR2 [4].

Biological context of GNAT3

• Among these proteins are several G protein alpha subunits, of which alpha gustducin is particularly interesting because of its similarity to the alpha transducins expressed in the visual system, suggesting that there are similarities in taste and visual transduction mechanisms [5].
• A multigene family of G-protein-coupled receptors, T2R (so-called TAS2R or TRB) receptors and a G-protein alpha subunit (Galpha), gustducin, are believed to be key molecules for its perception, but little is known about the molecular basis for its interaction [6].

Anatomical context of GNAT3

• Expression of the G-protein alpha-subunit gustducin in mammalian spermatozoa [7].

Associations of GNAT3 with chemical compounds

• Gustducin and transducin are guanine nucleotide binding regulatory proteins (G proteins) expressed in taste receptor cells and implicated in transducing taste cell responses to certain compounds that humans consider bitter or sweet [8].

Other interactions of GNAT3

• Gustducin, a taste-specific G-protein closely related to the transducins, is a key component in transducing the responses to compounds that humans equate with bitter and sweet [1].
• 5. Conversely, all synaptotagmin 1 expressing cells did not co-expressed type II cell markers, PLCbeta2, or gustducin [9].

Analytical, diagnostic and therapeutic context of GNAT3

• Western blot analysis demonstrated gustducin and PLC-beta(2) expression in the same samples, which was confirmed by immunocytochemistry [10].

References