Disease relevance of Inos

• The recombinant Drosophila INOS protein was expressed in Escherichia coli and the purified protein has proved to have a myo-inositol-1-phosphate synthase activity [1].

High impact information on Inos

• The purified INOS required NAD+ for the conversion of glucose-6-phosphate to inositol-1-phosphate [2].
• The putative active site residues are well conserved in Drosophila INOS protein [1].

Analytical, diagnostic and therapeutic context of Inos

• Southern blot analysis shows that Drosophila INOS gene is a single copy gene [1].
• Northern blot analysis reveals that Drosophila INOS gene expresses a 2.0-kb transcript that is more abundant in the head than the body, suggesting that it may be involved in brain function [1].
• The optimum pH for myo-inositol-1-phosphate synthase is 7.5, and the maximum activity was measured at 40 degrees C. The molecular weight of the native enzyme, as determined by gel filtration, was approximately Mr 271,000 +/- 15,000 [2].

References