High impact information on Gapdh1

• One of the genes encoding glyceraldehyde 3-phosphate dehydrogenase of Drosophila melanogaster, Gapdh-2, is expressed in all cell types examined, but its level of expression is regulated developmentally [1].
• In addition, the Gapdh-1 transcript appears highly enriched in the thorax section compared with the head and abdomen sections, while the Gapdh-2 transcript is evenly distributed [2].
• In addition, neither GAPDH nor aldolase colocalize at Z-discs and M-lines in the sarcomeres of muscles from GPDH-3 transgenic flies [3].
• Gapdh-1 lacks a sequence homologous to the TATA box in its -30-base pair region that is characteristic of many RNA polymerase II transcribed promoters [4].
• Transcription initiation sites as well as polyadenylation sites for both Gapdh transcripts have also been determined [4].

Biological context of Gapdh1

• Two Drosophila genes that code for the enzyme glyceraldehyde-3-phosphate dehydrogenase (Gapdh) have been isolated and their structures determined by DNA sequence analysis [4].
• Gapdh-1 is mapped by in situ hybridization at position 43E-F on the right arm of the second chromosome and Gapdh-2 at position 13F on the left arm of the X chromosome [4].
• The genome of this species has a single Gapdh gene which is localized at a cytogenetic position likely to be homologous to Gapdh-43 E of D. melanogaster [5].
• Gene amplification was used to determine which species have a Gapdh gene that is interrupted by an intron [5].

Associations of Gapdh1 with chemical compounds

• Immunoblots of sodium dodecyl sulfate gels were used to survey species from throughout the genus and results indicated that two classes of GAPDH subunits are present only in Drosophila species of the melanogaster and takahashi subgroups of the melanogaster group [5].

References