High impact information on KCNJ15

• A hyperprostaglandin E syndrome mutation in Kir1.1 (renal outer medullary potassium) channels reveals a crucial residue for channel function in Kir1.3 channels [1].
• Thus we postulate that in Kir1.3 channels the extracellular positively charged lysine is of crucial functional importance [1].
• Kir1.2 represents the human homolog of the rat BIRK-10 sequence, whereas Kir1.3 was unique compared with all available sequence data bases [2].
• Kir1.3 was most readily detected in kidney and was also expressed in pancreas > lung [2].
• In contrast, Kir1.3 expression in Xenopus oocytes was not detectable despite the fact that the cRNA efficiently directed the synthesis of a polypeptide of the expected Mr in an in vitro translation system [2].

Biological context of KCNJ15

• Isolation, mapping, and sequencing of trapped exons and captured cDNAs from cosmids of this region have revealed the presence of a gene (KCNJ15) encoding a potassium (K+) channel belonging to the family of inward rectifier K+ (Kir) channels [3].
• A new inward rectifier potassium channel gene (KCNJ15) localized on chromosome 21 in the Down syndrome chromosome region 1 (DCR1) [3].
• The genes that encode Kir1.2 and Kir1.3 were mapped to human chromosomes 1 and 21, respectively [2].
• Comparative analysis of the predicted amino acid sequences for Kir1.2 and Kir1.3 revealed they were 62% identical [2].
• Low stringency screening of a human kidney cDNA library and subsequent DNA sequence analysis identified two related K+ inward rectifier cDNAs, referred to as Kir1.2 and Kir1.3, which were derived from transcription of distinct human genes [2].

Anatomical context of KCNJ15

• Among members of the inwardly rectifying K(+) channel family, we found with reverse transcriptase-polymerase chain reaction analyses that Kir4.1, Kir4.2 and Kir7.1 were expressed in rat gastric mucosa [4].
• In hepatocyte couplets, Kir4.2 was predominantly localized to the basolateral membrane [5].

Other interactions of KCNJ15

• Cloning and characterization of two K+ inward rectifier (Kir) 1.1 potassium channel homologs from human kidney (Kir1.2 and Kir1.3) [2].

References