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Gene Review

HSP70  -  heat shock protein 70

Sus scrofa

Synonyms: HSP70B', HSPA6
 
 
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Disease relevance of HSP70

 

High impact information on HSP70

  • When primary cultures of guinea pig gastric mucosal cells were exposed to heat (43 degree C), ethanol, hydrogen peroxide (H2O2), or diamide, heat shock proteins (HSP90, HSP70, HSP60, and HSC73) were rapidly synthesized [6].
  • Gel mobility shift assay using a synthetic oligonucleotide coding HSP70 heat shock element showed that glutathione depletion inhibited the heat- and the reagent-initiated activation of the heat shock factor 1 (HSF1) and did not promote the expression of HSP70 mRNA [6].
  • Northern blot analysis demonstrated an increase in heat stress protein (HSP) 70 mRNA 2 h after the preconditioning ischemia; at this same time point, immunohistochemical analysis revealed a concentration of HSP70 in the nucleus and an overall increase in staining for HSP70 [5].
  • Amphetamine treatment was associated with the induction of mRNAs for HSP 27, HSP 70, and HSP 89 in all the vital organs, including heart, lung, liver, kidney, and brain [7].
  • Northern analysis showed no alterations in TnI or SR gene expression, but the stress protein HSP-70 was variably induced [8].
 

Chemical compound and disease context of HSP70

 

Biological context of HSP70

 

Anatomical context of HSP70

 

Associations of HSP70 with chemical compounds

  • Induction of tolerance to hyperosmotic stress, on the other hand, was associated with the cellular accumulation of osmolytes, such as amino acids, betaine and myo-inositol, and did not correlate with the induced expression of HSP70 [15].
  • In conclusion, the data show that the induction of HSP70 by Zn2+ attenuates the liberation of inflammatory mediators, as well as the course of hemodynamic variables due to LPS [16].
  • In contrast, coadministration of exogenous HSP-70 (1 mug/ml) potentiated dilation to cromakalim, CGRP, and NS-1619 [3].
  • Pretreatment with Zn2+ prior to lipopolysaccharide (LPS) infusion induced an increased heat shock protein 70 (HSP70) expression in the lungs, liver, and kidneys and significantly increased plasma levels of interleukin 6, 6-keto-PGF1 alpha, and thromboxane-B2, compared with untreated controls [16].
  • It has been reported that immunosuppressant cyclosporin A or FK506 binds to immunophilins in the cell and that these immunophilins make a complex with molecular chaperones HSP70 or HSP90 [17].
 

Other interactions of HSP70

  • Expression of IL-6 mRNA increased at 2 hours after reperfusion and HSP70 at 3 hours after reperfusion [1].
  • IL-2, IL-6, HSP70, and INF-gamma are mediators of the inflammatory process [1].
  • No significant changes were observed for HSP70, HSP90, and HO-2 [18].
  • The relative levels of alphaB-crystallin, HspB8, Hsp20, Hsp27, Hsp60, and Hsp70 as well as nitric oxide synthases (NOS) (endothelial NOS, inducible NOS, neuronal NOS) were examined by Western blot analysis [19].
  • Levels of IL-1, TNFalpha, IL-6, IL-10, TGF-1 beta and heat shock protein (HSP-70) were measured by ELISA in serum samples collected serially during the experiment and post-operatively [20].
 

Analytical, diagnostic and therapeutic context of HSP70

  • METHODS: Heat shock proteins (HSPs) were detected by immunoblotting with antibody against HSP90, HSP70, heat shock cognate protein 70, or HSP60 [21].
  • Embryos cultured under 20% O2 showed HSC70 levels significantly higher (P < 0.005) than embryos cultured under 5% O2, while heat shocked embryos presented HSP70 levels higher (P < 0.01) than control group [22].
  • Southern blot hybridization with the 5' section, the central section, and the 3' section of the 2.6-kb probe and also with a swine 4.5-kb HSP70 genomic probe suggested the existence, within the overlapping clones, of three distinct HSP70 sequences encompassing a segment no longer than 22 kb [23].
  • HSP70 was visualized by immunohistochemistry [24].
  • Genomic DNA isolated from 55 boars (41 Duroc, nine Landrace, and five Yorkshire) was subjected to PCR amplification of the 5'-flanking region of HSP70 [25].

References

  1. Kinetics and localization of interleukin-2, interleukin-6, heat shock protein 70, and interferon gamma during intestinal-rerfusion injury. Braun, F., Hosseini, M., Wieland, E., Sattler, B., Müller, A.R., Fändrich, F., Kremer, B., Ringe, B. Transplant. Proc. (2004) [Pubmed]
  2. Influence of heat shock protein 70 and metallothionein induction by zinc-bis-(DL-hydrogenaspartate) on the release of inflammatory mediators in a porcine model of recurrent endotoxemia. Klosterhalfen, B., Töns, C., Hauptmann, S., Tietze, L., Offner, F.A., Küpper, W., Kirkpatrick, C.J. Biochem. Pharmacol. (1996) [Pubmed]
  3. Heat shock protein modulation of KATP and KCa channel cerebrovasodilation after brain injury. Armstead, W.M., Hecker, J.G. Am. J. Physiol. Heart Circ. Physiol. (2005) [Pubmed]
  4. Overexpression of alphaB crystallin in the gastrointestinal tract of the newborn piglet after hypoxia. Nefti, O., Grongnet, J.F., David, J.C. Shock (2005) [Pubmed]
  5. Late preconditioning against myocardial stunning. An endogenous protective mechanism that confers resistance to postischemic dysfunction 24 h after brief ischemia in conscious pigs. Sun, J.Z., Tang, X.L., Knowlton, A.A., Park, S.W., Qiu, Y., Bolli, R. J. Clin. Invest. (1995) [Pubmed]
  6. Glutathione depletion impairs transcriptional activation of heat shock genes in primary cultures of guinea pig gastric mucosal cells. Rokutan, K., Hirakawa, T., Teshima, S., Honda, S., Kishi, K. J. Clin. Invest. (1996) [Pubmed]
  7. Drug-induced heat-shock preconditioning improves postischemic ventricular recovery after cardiopulmonary bypass. Maulik, N., Engelman, R.M., Wei, Z., Liu, X., Rousou, J.A., Flack, J.E., Deaton, D.W., Das, D.K. Circulation (1995) [Pubmed]
  8. Absence of troponin I degradation or altered sarcoplasmic reticulum uptake protein expression after reversible ischemia in swine. Thomas, S.A., Fallavollita, J.A., Lee, T.C., Feng, J., Canty, J.M. Circ. Res. (1999) [Pubmed]
  9. Indomethacin attenuates early increases in inducible heat shock protein 70 after cerebral ischemia/reperfusion in piglets. Beasley, T.C., Bari, F., Thore, C., Thrikawala, N., Louis, T., Busija, D. Brain Res. Dev. Brain Res. (1998) [Pubmed]
  10. Heat shock protein 70, heat shock protein 32, and vascular endothelial growth factor production and their effects on lipopolysaccharide-induced apoptosis in porcine aortic endothelial cells. Bernardini, C., Zannoni, A., Turba, M.E., Fantinati, P., Tamanini, C., Bacci, M.L., Forni, M. Cell Stress Chaperones (2005) [Pubmed]
  11. Hsp27, Hsp70, and metallothionein in MDCK and LLC-PK1 renal epithelial cells: effects of prolonged exposure to cadmium. Bonham, R.T., Fine, M.R., Pollock, F.M., Shelden, E.A. Toxicol. Appl. Pharmacol. (2003) [Pubmed]
  12. Acceleration of hepatocellular energy by idebenone during early reperfusion after cold preservation ameliorates heat shock protein 70 gene expression in a pig liver model. Schütz, E., Wieland, E., Heine, L., Hensel, A., Schmiedl, A., Armstrong, V.W., Richter, J., Schuff-Werner, P., Günther, E., Oellerich, M. Transplantation (1997) [Pubmed]
  13. Serum thymic factor, FTS, attenuates cisplatin nephrotoxicity by suppressing cisplatin-induced ERK activation. Kohda, Y., Kawai, Y., Iwamoto, N., Matsunaga, Y., Aiga, H., Awaya, A., Gemba, M. Biochem. Pharmacol. (2005) [Pubmed]
  14. Weaning affects the expression of heat shock proteins in different regions of the gastrointestinal tract of piglets. David, J.C., Grongnet, J.F., Lalles, J.P. J. Nutr. (2002) [Pubmed]
  15. Roles of compatible osmolytes and heat shock protein 70 in the induction of tolerance to stresses in porcine endothelial cells. Alfieri, R.R., Petronini, P.G., Bonelli, M.A., Desenzani, S., Cavazzoni, A., Borghetti, A.F., Wheeler, K.P. J. Physiol. (Lond.) (2004) [Pubmed]
  16. The influence of heat shock protein 70 induction on hemodynamic variables in a porcine model of recurrent endotoxemia. Klosterhalfen, B., Hauptmann, S., Tietze, L., Töns, C., Winkeltau, G., Küpper, W., Kirkpatrick, C.J. Shock (1997) [Pubmed]
  17. Mammalian HSP60 is a major target for an immunosuppressant mizoribine. Itoh, H., Komatsuda, A., Wakui, H., Miura, A.B., Tashima, Y. J. Biol. Chem. (1999) [Pubmed]
  18. Effects of hypoxia on stress proteins in the piglet brain at birth. Chiral, M., Grongnet, J.F., Plumier, J.C., David, J.C. Pediatr. Res. (2004) [Pubmed]
  19. Effects of hypoxia on stress proteins in the piglet heart at birth. Louapre, P., Grongnet, J.F., Tanguay, R.M., David, J.C. Cell Stress Chaperones (2005) [Pubmed]
  20. Induction of profound hypothermia modulates the immune/inflammatory response in a swine model of lethal hemorrhage. Chen, Z., Chen, H., Rhee, P., Koustova, E., Ayuste, E.C., Honma, K., Nadel, A., Alam, H.B. Resuscitation. (2005) [Pubmed]
  21. Geranylgeranylacetone induces heat shock proteins in cultured guinea pig gastric mucosal cells and rat gastric mucosa. Hirakawa, T., Rokutan, K., Nikawa, T., Kishi, K. Gastroenterology (1996) [Pubmed]
  22. Expression of HSP70/HSC70 in swine blastocysts: effects of oxidative and thermal stress. Bernardini, C., Fantinati, P., Zannoni, A., Forni, M., Tamanini, C., Bacci, M.L. Mol. Reprod. Dev. (2004) [Pubmed]
  23. Isolation of four HSP70 genes in the pig and localization on chromosomes 7 and 14. Nunes, M., Yerle, M., Dezeure, F., Gellin, J., Chardon, P., Vaiman, M. Mamm. Genome (1993) [Pubmed]
  24. Effect of zinc pretreatment on pulmonary endothelial cells in vitro and pulmonary function in a porcine model of endotoxemia. Krones, C.J., Klosterhalfen, B., Butz, N., Hoelzl, F., Junge, K., Stumpf, M., Peiper, C., Klinge, U., Schumpelick, V. J. Surg. Res. (2005) [Pubmed]
  25. Effects of single nucleotide polymorphisms in the 5'-flanking region of heat shock protein 70.2 gene on semen quality in boars. Huang, S.Y., Chen, M.Y., Lin, E.C., Tsou, H.L., Kuo, Y.H., Ju, C.C., Lee, W.C. Anim. Reprod. Sci. (2002) [Pubmed]
 
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