High impact information on Xbub3

• The mitotic checkpoint prevents advance to anaphase prior to successful attachment of every centromere/kinetochore to mitotic spindle microtubules [1].
• The mitotic checkpoint ensures that chromosomes are divided equally between daughter cells and is a primary mechanism preventing the chromosome instability often seen in aneuploid human tumors [2].
• The mitotic checkpoint is the major cell cycle control mechanism for maintaining chromosome content in multicellular organisms [3].
• ZW10 links mitotic checkpoint signaling to the structural kinetochore [2].
• Expression of Xbub3 was observed from oocyte to tadpole [4].

Biological context of Xbub3

• In the cleavage-stage embryos, the maternal transcripts of Xbub3 were recruited into each blastomere, associating closely with chromosomes [4].
• A novel gene, Xbub3, was cloned by screening the oocyte cDNA library with an animal blastomere-enriched PCR product [4].
• Whole-mount in situ hybridization showed that Xbub3 mRNAs were uniformly distributed in the early stages of oogenesis but gradually localized to the animal hemisphere, especially in the perinuclear cytoplasm of full-grown oocytes [4].
• The mitotic checkpoint acts to inhibit entry into anaphase until all chromosomes have successfully attached to spindle microtubules [5].
• The mitotic checkpoint blocks the activation of the anaphase-promoting complex (APC) until all sister chromatids have achieved bipolar attachment to the spindle [6].

Anatomical context of Xbub3

• Zygotic expression of Xbub3 was widely detected in gastrula ectoderm and was gradually restricted to the central nervous systems, eyes, and branchial arches by the tadpole stage [4].

Associations of Xbub3 with chemical compounds

• One such cell cycle delay is invoked by microtubule-targeting agents such as nocodazole or paclitaxel (Taxol) and is mediated by mitotic checkpoint proteins that include BubR1 [7].

References