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Gene Review

LMNB1  -  lamin B1

Homo sapiens

Synonyms: ADLD, LMN, LMN2, LMNB, Lamin-B1
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Disease relevance of LMNB1

  • Using a monoclonal autoantibody (72B9) derived from a murine lupus strain, we have cloned a cDNA from the human T-cell line MOLT-4, which encodes nuclear lamin B. The identity of the encoded protein as lamin B was established by both biochemical and immunological criteria [1].
  • The ability of purified nuclear lamin A, lamin B, lamin C, and vimentin from Ehrlich ascites tumor cells to bind nucleic acids was investigated in vitro via a quantitative filter binding assay [2].
  • Epstein-Barr virus nuclear antigen-1 colocalizes with lamin B1 in the nucleoplasm and along the nuclear rim [3].
  • In the small cell lung cancer lines OH-1, OH-3, NCI-H82, NCI-H209, and NCI-H249, levels of lamin B were similar to those observed in the non-small cell lines, but the levels of lamins A and C were diminished by greater than or equal to 80% [4].
  • Treatment with VES induced membrane translocation of Fas; cleavages of caspase-3, PARP, and lamin B; hypophosphorylation of retinoblastoma protein; and increase of p21(WAF1) protein level [5].

High impact information on LMNB1


Chemical compound and disease context of LMNB1


Biological context of LMNB1


Anatomical context of LMNB1


Associations of LMNB1 with chemical compounds

  • While lamin B is cleaved by caspase 6, the protease responsible for the cleavage of LAP2 and Nup153 was probably caspase 3, since (1) cleavage of both proteins was specifically prevented by in vivo addition of caspase 3 inhibitor Ac-DEVD-CHO and (2) consensus sites for these caspases are present in both proteins [11].
  • The heterologous lamin B was extracted at 8 M urea, but not at 4 M urea, thus behaving as a peripheral membrane protein and indistinguishable from assembled lamins [16].
  • Inspection of the deduced amino acid sequence of lamin B revealed the presence in coil 1B of the alpha-helical domain of a leucine heptad repeat region [1].
  • In vitro translation with the cDNA clone revealed an EDTA-sensitive posttranslational modification which resulted in an increase in the apparent molecular weight to that equivalent to the native in vivo-synthesized lamin B protein [1].
  • By monitoring intracellular expression of vimentin and lamin B1 during SA, we found that these two proteins were cleaved and that such cleavage was reversed by the pan caspase inhibitor N-benzyloxy-carbonyl-V-A-D-O-methylfluoromethyl ketone (z-VAD-fmk) [17].

Physical interactions of LMNB1


Co-localisations of LMNB1

  • A second domain sufficient to bind HA95 colocalizes with the lamin B-binding domain of LAP2beta at residues 299-373 [22].

Regulatory relationships of LMNB1

  • The protease responsible appears to be calcium-dependent and the concomitant cleavage of PARP and p53 was consistent with a beta-lap-mediated activation of calpain. beta-Lap exposure also stimulated the cleavage of lamin B, a putative caspase 6 substrate [23].
  • Bcl-2 prevents CD95 (Fas/APO-1)-induced degradation of lamin B and poly(ADP-ribose) polymerase and restores the NF-kappaB signaling pathway [24].
  • Effector caspases such as caspase-3 are transiently activated through the mitochondrial pathway during erythroblast differentiation and cleave proteins involved in nucleus integrity (lamin B) and chromatin condensation (acinus)without inducing cell death and cleavage of GATA-1 [25].

Other interactions of LMNB1


Analytical, diagnostic and therapeutic context of LMNB1


  1. In vitro posttranslational modification of lamin B cloned from a human T-cell line. Pollard, K.M., Chan, E.K., Grant, B.J., Sullivan, K.F., Tan, E.M., Glass, C.A. Mol. Cell. Biol. (1990) [Pubmed]
  2. The in vitro DNA-binding properties of purified nuclear lamin proteins and vimentin. Shoeman, R.L., Traub, P. J. Biol. Chem. (1990) [Pubmed]
  3. Epstein-Barr virus nuclear antigen-1 colocalizes with lamin B1 in the nucleoplasm and along the nuclear rim. Ito, S., Eda, H., Ban, F., Yanagi, K. Arch. Virol. (2003) [Pubmed]
  4. Differential expression of nuclear envelope lamins A and C in human lung cancer cell lines. Kaufmann, S.H., Mabry, M., Jasti, R., Shaper, J.H. Cancer Res. (1991) [Pubmed]
  5. Activation of PKC but not of ERK is required for vitamin E-succinate-induced apoptosis of HL-60 cells. Bang, O.S., Park, J.H., Kang, S.S. Biochem. Biophys. Res. Commun. (2001) [Pubmed]
  6. Lamin B1 duplications cause autosomal dominant leukodystrophy. Padiath, Q.S., Saigoh, K., Schiffmann, R., Asahara, H., Yamada, T., Koeppen, A., Hogan, K., Pt??cek, L.J., Fu, Y.H. Nat. Genet. (2006) [Pubmed]
  7. Network antibodies identify nuclear lamin B as a physiological attachment site for peripherin intermediate filaments. Djabali, K., Portier, M.M., Gros, F., Blobel, G., Georgatos, S.D. Cell (1991) [Pubmed]
  8. Lamin B, caspase-3 activity, and apoptosis induction by a combination of HMG-CoA reductase inhibitor and COX-2 inhibitors: a novel approach in developing effective chemopreventive regimens. Swamy, M.V., Cooma, I., Reddy, B.S., Rao, C.V. Int. J. Oncol. (2002) [Pubmed]
  9. Anti-laminin auto antibodies in ANCA-associated vasculitis. Vecchi, M.L., Radice, A., Renda, F., Mulé, G., Sinico, R.A. Nephrol. Dial. Transplant. (2000) [Pubmed]
  10. Single-nucleotide polymorphisms of the nuclear lamina proteome. Hegele, R.A., Yuen, J., Cao, H. J. Hum. Genet. (2001) [Pubmed]
  11. Caspase-dependent proteolysis of integral and peripheral proteins of nuclear membranes and nuclear pore complex proteins during apoptosis. Buendia, B., Santa-Maria, A., Courvalin, J.C. J. Cell. Sci. (1999) [Pubmed]
  12. A carboxyl-terminal interaction of lamin B1 is dependent on the CAAX endoprotease Rce1 and carboxymethylation. Maske, C.P., Hollinshead, M.S., Higbee, N.C., Bergo, M.O., Young, S.G., Vaux, D.J. J. Cell Biol. (2003) [Pubmed]
  13. Human laminopathies: nuclei gone genetically awry. Capell, B.C., Collins, F.S. Nat. Rev. Genet. (2006) [Pubmed]
  14. Distribution of emerin and lamins in the heart and implications for Emery-Dreifuss muscular dystrophy. Manilal, S., Sewry, C.A., Pereboev, A., Man, N., Gobbi, P., Hawkes, S., Love, D.R., Morris, G.E. Hum. Mol. Genet. (1999) [Pubmed]
  15. Targeting of membranes to sea urchin sperm chromatin is mediated by a lamin B receptor-like integral membrane protein. Collas, P., Courvalin, J.C., Poccia, D. J. Cell Biol. (1996) [Pubmed]
  16. Colocalization of vertebrate lamin B and lamin B receptor (LBR) in nuclear envelopes and in LBR-induced membrane stacks of the yeast Saccharomyces cerevisiae. Smith, S., Blobel, G. Proc. Natl. Acad. Sci. U.S.A. (1994) [Pubmed]
  17. Cell surface expression of intermediate filament proteins vimentin and lamin B1 in human neutrophil spontaneous apoptosis. Moisan, E., Girard, D. J. Leukoc. Biol. (2006) [Pubmed]
  18. Primary structure analysis and lamin B and DNA binding of human LBR, an integral protein of the nuclear envelope inner membrane. Ye, Q., Worman, H.J. J. Biol. Chem. (1994) [Pubmed]
  19. Vimentin-associated mitotic vesicles interact with chromosomes in a lamin B- and phosphorylation-dependent manner. Maison, C., Pyrpasopoulou, A., Georgatos, S.D. EMBO J. (1995) [Pubmed]
  20. Lamin-binding fragment of LAP2 inhibits increase in nuclear volume during the cell cycle and progression into S phase. Yang, L., Guan, T., Gerace, L. J. Cell Biol. (1997) [Pubmed]
  21. Protein kinase C-beta II Is an apoptotic lamin kinase in polyomavirus-transformed, etoposide-treated pyF111 rat fibroblasts. Chiarini, A., Whitfield, J.F., Armato, U., Dal Pra, I. J. Biol. Chem. (2002) [Pubmed]
  22. HA95 and LAP2 beta mediate a novel chromatin-nuclear envelope interaction implicated in initiation of DNA replication. Martins, S., Eikvar, S., Furukawa, K., Collas, P. J. Cell Biol. (2003) [Pubmed]
  23. Activation of a cysteine protease in MCF-7 and T47D breast cancer cells during beta-lapachone-mediated apoptosis. Pink, J.J., Wuerzberger-Davis, S., Tagliarino, C., Planchon, S.M., Yang, X., Froelich, C.J., Boothman, D.A. Exp. Cell Res. (2000) [Pubmed]
  24. Bcl-2 prevents CD95 (Fas/APO-1)-induced degradation of lamin B and poly(ADP-ribose) polymerase and restores the NF-kappaB signaling pathway. Mandal, M., Maggirwar, S.B., Sharma, N., Kaufmann, S.H., Sun, S.C., Kumar, R. J. Biol. Chem. (1996) [Pubmed]
  25. Caspase activation is required for terminal erythroid differentiation. Zermati, Y., Garrido, C., Amsellem, S., Fishelson, S., Bouscary, D., Valensi, F., Varet, B., Solary, E., Hermine, O. J. Exp. Med. (2001) [Pubmed]
  26. Expression and localization of nuclear proteins in autosomal-dominant Emery-Dreifuss muscular dystrophy with LMNA R377H mutation. Reichart, B., Klafke, R., Dreger, C., Krüger, E., Motsch, I., Ewald, A., Schäfer, J., Reichmann, H., Müller, C.R., Dabauvalle, M.C. BMC Cell Biol. (2004) [Pubmed]
  27. SNP identification, linkage and radiation hybrid mapping of the porcine lamin A/C (LMNA) gene to chromosome 4q. Wagenknecht, D., Stratil, A., Bartenschlager, H., Van Poucke, M., Peelman, L.J., Majzlík, I., Geldermann, H. J. Anim. Breed. Genet. (2006) [Pubmed]
  28. Identification of nuclear beta II protein kinase C as a mitotic lamin kinase. Goss, V.L., Hocevar, B.A., Thompson, L.J., Stratton, C.A., Burns, D.J., Fields, A.P. J. Biol. Chem. (1994) [Pubmed]
  29. Retinoic acid induction of nuclear envelope-limited chromatin sheets in HL-60. Olins, A.L., Buendia, B., Herrmann, H., Lichter, P., Olins, D.E. Exp. Cell Res. (1998) [Pubmed]
  30. Expression of nuclear lamins in human tissues and cancer cell lines and transcription from the promoters of the lamin A/C and B1 genes. Lin, F., Worman, H.J. Exp. Cell Res. (1997) [Pubmed]
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