Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

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Gene: asf1 - anti-silencing factor 1

Drosophila melanogaster

Synonyms: Anti-silencing function protein 1, ASF1, CG9383, dASF1, dASF/SF2, Histone chaperone asf1, RCAF subunit ASF1, Replication-coupling assembly factor subunit ASF1

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Schulz, L.L., Ge, H., Carrera, P., Tyler, J.K., Tyler, J.K.

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Disease relevance of asf1

ASF-1 produced in E. coli can activate splicing in vitro and switch 5' splice-site utilization, establishing that the recombinant protein is sufficient to supply these activities [1].

High impact information on asf1

Use of the upstream site results in a second mRNA (ASF-2) in which translation of the downstream exon occurs extensively in an alternative reading frame distinct from ASF-1 [1].
ASF-1 consists of 248 amino acid residues, including an 80 residue RNA-binding domain at its N-terminus and a 50 residue C-terminal region that is 80% serine plus arginine [1].
Genetic analyses in Saccharomyces cerevisiae demonstrate that ASF1 is essential for normal cell cycle progression, and suggest that RCAF mediates chromatin assembly after DNA replication and the repair of double-strand DNA damage in vivo [2].
In mammals, their activity peaks during S phase, when they phosphorylate the antisilencing function protein 1 (ASF1), a histone chaperone involved in replication-dependent chromatin assembly [3].
Here, we show that Drosophila ASF1 is also a phosphorylation target of TLK, and that the two components cooperate to control chromatin replication in vivo [3].

Biological context of asf1

Interaction between the Drosophila CAF-1 and ASF1 chromatin assembly factors [4].
The histone chaperone ASF1 localizes to active DNA replication forks to mediate efficient DNA replication [5].
Cells lacking dASF1 accumulate in S phase of the cell cycle as determined by flow cytometry analysis of DNA content and quantitation of the proportion of cells with replication foci [5].

Associations of asf1 with chemical compounds

In agreement, bromodeoxyuridine (BrdU) pulse-chase analysis demonstrates that the absence of ASF1 leads to delayed progression through S-phase [5].
We have also found that dASF1 colocalizes with DNA replication foci throughout S phase by immunofluorescence analysis and that these dASF1 foci are disrupted upon inhibition of DNA replication by treatment of cells with hydroxyurea [5].

Other interactions of asf1

The analysis of native and recombinant dCAF-1 revealed an interaction between dCAF-1 and the Drosophila anti-silencing function 1 (dASF1) component of replication-coupling assembly factor (RCAF) [4].
The assembly and disassembly of chromatin structures from histone proteins and DNA are mediated by histone chaperones, including the histone H3/H4 chaperone anti-silencing function 1 (ASF1) [5].

References

Primary structure of the human splicing factor ASF reveals similarities with Drosophila regulators. Ge, H., Zuo, P., Manley, J.L. Cell (1991)
The RCAF complex mediates chromatin assembly during DNA replication and repair. Tyler, J.K., Adams, C.R., Chen, S.R., Kobayashi, R., Kamakaka, R.T., Kadonaga, J.T. Nature (1999)
Tousled-like kinase functions with the chromatin assembly pathway regulating nuclear divisions. Carrera, P., Moshkin, Y.M., Gronke, S., Sillje, H.H., Nigg, E.A., Jackle, H., Karch, F. Genes Dev. (2003)
Interaction between the Drosophila CAF-1 and ASF1 chromatin assembly factors. Tyler, J.K., Collins, K.A., Prasad-Sinha, J., Amiott, E., Bulger, M., Harte, P.J., Kobayashi, R., Kadonaga, J.T. Mol. Cell. Biol. (2001)
The histone chaperone ASF1 localizes to active DNA replication forks to mediate efficient DNA replication. Schulz, L.L., Tyler, J.K. FASEB J. (2006)