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Gene Review

pum  -  pumilio

Drosophila melanogaster

Synonyms: CG9755, CG9763, Dmel\CG9755, EP(3)0883, Maternal protein pumilio, ...
 
 
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Disease relevance of pum

 

High impact information on pum

  • These and other observations suggest that pum acts by recognizing the NRE and then recruiting nos [4].
  • Binding of pumilio to maternal hunchback mRNA is required for posterior patterning in Drosophila embryos [4].
  • Pumilio controls germline stem cells in Drosophila females, and, in lower eukaryotes, PUF proteins promote continued mitoses [5].
  • The Drosophila gene pumilio is expressed maternally, and its function is essential during early embryogenesis for the formation of abdominal segments [6].
  • Puf proteins regulate translation and mRNA stability by binding sequences in their target RNAs through the Pumilio homology domain (PUM-HD), which is characterized by eight tandem copies of a 36 amino acid motif, the PUM repeat [7].
 

Biological context of pum

 

Anatomical context of pum

  • Nanos and Pumilio have critical roles in the development and function of Drosophila germline stem cells [9].
  • First, a mutation in pum causes a defect in pole-cell migration into the gonads [10].
  • We also show that Nos and Pum can repress cap-independent translation from an internal ribosome entry site (IRES) in vivo, suggesting that they act downstream of the initial steps of normal, cap-dependent translation [11].
  • Here, we show that Pum is an important mediator of synaptic growth and plasticity at the neuromuscular junction (NMJ) [12].
  • These studies demonstrate that evolutionarily distant organisms utilize conserved factors to regulate early germ cell development and survival, and that these factors include members of the nanos and pumilio gene families [13].
 

Associations of pum with chemical compounds

 

Physical interactions of pum

  • In this report we use protein-protein and protein-RNA interaction assays in yeast and in vitro to show that Nanos forms a ternary complex with the RNA-binding domain of Pumilio and the NRE [15].
  • The PUMILIO (PUM) protein is thought to bind the NREs and thereby repress hb translation [16].
 

Regulatory relationships of pum

  • These data demonstrate that the poly(A) tail is not required for regulation and suggest that NOS and PUM can regulate hb translation by two mechanisms: removal of the poly(A) tail and a poly(A)-independent pathway that directly affects translation [17].
  • We demonstrate that the prototypical Puf protein Pumilio temporally regulates bicoid (bcd) mRNA translation via evolutionarily conserved Nanos response elements (NRE) in its 3'UTR [18].
  • We show that Pum inhibits pole-cell division by repressing translation of cyclin B messenger RNA [10].
  • The translational repressor Pumilio regulates presynaptic morphology and controls postsynaptic accumulation of translation factor eIF-4E [12].
  • Maternal Nanos and Pumilio regulate zygotic vasa expression autonomously in the germ-line progenitors of Drosophila melanogaster embryos [19].
 

Other interactions of pum

  • While pumilio mutant ovaries fail to maintain stem cells and all germline cells differentiate into egg chambers, the focus of nanos function seems to lie in the differentiation of the stem cell progeny, the cystoblast [9].
  • Phenotypically, embryos from transgenic mothers that harbor bcd NRE mutations exhibited dominant anterior patterning defects and we discovered similar head defects in embryos from pum(-) mothers [18].
  • Regulatory relationship among piwi, pumilio, and bag-of-marbles in Drosophila germline stem cell self-renewal and differentiation [20].
  • These results, together with genetic epistasis studies, suggest that postsynaptic Pum modulates synaptic function via direct control of eIF-4E expression [12].
  • In cystoblasts, accumulating Bam protein antagonizes pumilio, permitting the translation of cystoblast-promoting transcripts [21].
 

Analytical, diagnostic and therapeutic context of pum

  • We generated transgenic flies expressing affinity-tagged PUMILIO under the control of an ovary-specific promoter, and we purified PUMILIO from whole adult flies and embryos and analyzed associated mRNAs by using DNA microarrays [22].

References

  1. The Drosophila melanogaster translational repressor pumilio regulates neuronal excitability. Schweers, B.A., Walters, K.J., Stern, M. Genetics (2002) [Pubmed]
  2. Transmission of the Diachasmimorpha longicaudata rhabdovirus (DlRhV) to wasp offspring: an ultrastructural analysis. Lawrence, P.O., Matos, L.F. J. Insect Physiol. (2005) [Pubmed]
  3. Gene insertion: current progress and long-term goals. Crittenden, L.B., Salter, D.W. Avian Dis. (1986) [Pubmed]
  4. Binding of pumilio to maternal hunchback mRNA is required for posterior patterning in Drosophila embryos. Murata, Y., Wharton, R.P. Cell (1995) [Pubmed]
  5. A conserved RNA-binding protein controls germline stem cells in Caenorhabditis elegans. Crittenden, S.L., Bernstein, D.S., Bachorik, J.L., Thompson, B.E., Gallegos, M., Petcherski, A.G., Moulder, G., Barstead, R., Wickens, M., Kimble, J. Nature (2002) [Pubmed]
  6. Pumilio is essential for function but not for distribution of the Drosophila abdominal determinant Nanos. Barker, D.D., Wang, C., Moore, J., Dickinson, L.K., Lehmann, R. Genes Dev. (1992) [Pubmed]
  7. Crystal structure of a Pumilio homology domain. Wang, X., Zamore, P.D., Hall, T.M. Mol. Cell (2001) [Pubmed]
  8. Nanos and Pumilio are essential for dendrite morphogenesis in Drosophila peripheral neurons. Ye, B., Petritsch, C., Clark, I.E., Gavis, E.R., Jan, L.Y., Jan, Y.N. Curr. Biol. (2004) [Pubmed]
  9. Nanos and Pumilio have critical roles in the development and function of Drosophila germline stem cells. Forbes, A., Lehmann, R. Development (1998) [Pubmed]
  10. Maternal Pumilio acts together with Nanos in germline development in Drosophila embryos. Asaoka-Taguchi, M., Yamada, M., Nakamura, A., Hanyu, K., Kobayashi, S. Nat. Cell Biol. (1999) [Pubmed]
  11. The Pumilio RNA-binding domain is also a translational regulator. Wharton, R.P., Sonoda, J., Lee, T., Patterson, M., Murata, Y. Mol. Cell (1998) [Pubmed]
  12. The translational repressor Pumilio regulates presynaptic morphology and controls postsynaptic accumulation of translation factor eIF-4E. Menon, K.P., Sanyal, S., Habara, Y., Sanchez, R., Wharton, R.P., Ramaswami, M., Zinn, K. Neuron (2004) [Pubmed]
  13. nos-1 and nos-2, two genes related to Drosophila nanos, regulate primordial germ cell development and survival in Caenorhabditis elegans. Subramaniam, K., Seydoux, G. Development (1999) [Pubmed]
  14. The Drosophila pumilio gene: an unusually long transcription unit and an unusual protein. Macdonald, P.M. Development (1992) [Pubmed]
  15. Recruitment of Nanos to hunchback mRNA by Pumilio. Sonoda, J., Wharton, R.P. Genes Dev. (1999) [Pubmed]
  16. The PUMILIO-RNA interaction: a single RNA-binding domain monomer recognizes a bipartite target sequence. Zamore, P.D., Bartel, D.P., Lehmann, R., Williamson, J.R. Biochemistry (1999) [Pubmed]
  17. Poly(A)-independent regulation of maternal hunchback translation in the Drosophila embryo. Chagnovich, D., Lehmann, R. Proc. Natl. Acad. Sci. U.S.A. (2001) [Pubmed]
  18. An anterior function for the Drosophila posterior determinant Pumilio. Gamberi, C., Peterson, D.S., He, L., Gottlieb, E. Development (2002) [Pubmed]
  19. Maternal Nanos and Pumilio regulate zygotic vasa expression autonomously in the germ-line progenitors of Drosophila melanogaster embryos. Sano, H., Mukai, M., Kobayashi, S. Dev. Growth Differ. (2001) [Pubmed]
  20. Regulatory relationship among piwi, pumilio, and bag-of-marbles in Drosophila germline stem cell self-renewal and differentiation. Szakmary, A., Cox, D.N., Wang, Z., Lin, H. Curr. Biol. (2005) [Pubmed]
  21. Gene circuitry controlling a stem cell niche. Chen, D., McKearin, D. Curr. Biol. (2005) [Pubmed]
  22. Genome-wide identification of mRNAs associated with the translational regulator PUMILIO in Drosophila melanogaster. Gerber, A.P., Luschnig, S., Krasnow, M.A., Brown, P.O., Herschlag, D. Proc. Natl. Acad. Sci. U.S.A. (2006) [Pubmed]
 
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