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Gene: Brf  -  Brf

Drosophila melanogaster

Synonyms: BRF, BRF1, CG31256, CG4155, CG5419, TFIIIB
 
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High impact information on Brf

  • In vivo, the majority of TRF1 is complexed with BRF and these two proteins colocalize at many polytene chromosome sites containing RNA pol III genes [1].
  • Together, these results indicate that the TPA response in Drosophila cells stimulates specific transcription of RNA polymerase III genes by increasing the activity of the limiting transcription component, TFIIIB, and thereby increasing the number of functional transcription complexes [2].
  • Finally, the data suggest that transcription factors TFIID and TFIIIB, although both capable of binding a variety of TATA elements, have distinct sequence preferences for recognizing the TATA box and possibly the surrounding DNA [3].
  • Bombyx BRF contains the following domains that have been noted in all other BRFs, and that are likely, therefore, to provide highly conserved functions: a zinc finger domain, an imperfect repeat, three "BRF Homology" domains, and an acidic domain at the C-terminus [4].
  • To identify the protein domains responsible for its conserved and specialized functions, putative TFIIB-Related Factor (BRF) from the silkworm (Bombyx mori) was compared with BRFs from other organisms [4].
 

Analytical, diagnostic and therapeutic context of Brf

  • Western blot analysis of the TATA-binding protein subunit of TFIIIB revealed that there is an increase in the amount of this polypeptide present in the induced cell extracts and TFIIIB fraction [2].
  • A protein (far Western) blot analysis revealed that dTAFIII105, present in a TFIIIB fraction, directly interacts with TATA-binding protein (TBP) [5].

References

  1. A TRF1:BRF complex directs Drosophila RNA polymerase III transcription. Takada, S., Lis, J.T., Zhou, S., Tjian, R. Cell (2000)
  2. Induction of Drosophila RNA polymerase III gene expression by the phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA) is mediated by transcription factor IIIB. Garber, M.E., Vilalta, A., Johnson, D.L. Mol. Cell. Biol. (1994)
  3. Role of TATA box sequence and orientation in determining RNA polymerase II/III transcription specificity. Wang, Y., Jensen, R.C., Stumph, W.E. Nucleic Acids Res. (1996)
  4. Cloning of a putative Bombyx mori TFIIB-related factor (BRF). Martinez, M.J., Sprague, K.U. Arch. Insect Biochem. Physiol. (2003)
  5. An RNA polymerase III-defective mutation in TATA-binding protein disrupts its interaction with a transcription factor IIIB subunit in drosophila cells. Vilalta, A., Trivedi, A., Wang, Z., Roeder, R.G., Johnson, D.L. J. Biol. Chem. (1997)
 
 
 
 
 
 
 
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