Disease relevance of LOC500959

• Hypoxia up-regulates triosephosphate isomerase expression via a HIF-dependent pathway [1].
• Analyses of polypeptides in the liver of a novel mutant (LEC rats) to hereditary hepatitis and hepatoma by two-dimensional gel electrophoresis: identification of P29/6.8 as carbonic anhydrase III and triosephosphate isomerase [2].

High impact information on LOC500959

• Northern blot analysis showed that the TPI cDNA hybridized with two transcripts in testis [3].
• Expression of triosephosphate isomerase transcripts in rat testis: evidence for retinol regulation and a novel germ cell transcript [3].
• Furthermore, screening of an adult rat testis cDNA library with the TPI cDNA yielded a cDNA putatively corresponding to the 1.5-kb transcript [3].
• These results suggest independent processing mechanisms for TPI expression in the somatic cells and the haploid germ cells of testis [3].
• Sequence analyses of the two TPI cDNAs revealed a 100-bp deletion in one cDNA that may be due to use of an alternative polyadenylation signal [3].

Biological context of LOC500959

• Additional factors include the rate of glycerol metabolism relative to the rate of triosephosphate isomerase, isotope dilution in the Krebs cycle, equilibration by the fumarase reaction, flux through pyruvate dehydrogenase, and recycling via pyruvate kinase [4].

Anatomical context of LOC500959

• Identification of HNE modified proteins in two alternative model systems, human retinal pigment epithelial cells in culture (ARPE19) and human donor eyes, indicated that triosephosphate isomerase and alpha enolase are generally modified [5].

Associations of LOC500959 with chemical compounds

• The utilization of inorganic pyrophosphate is measured by coupling the production of fructose-1,6-bisphosphate with the oxidation of NADH using fructose-bisphosphate aldolase (EC 4.1.2.13), triosephosphate isomerase (EC 5.3.1.1), and glycerol-3-phosphate dehydrogenase (NAD+)(EC 1.1.1.8) [6].
• Consistently, the results of differential display suggested the induction of glycolytic enzymes, including aldolase A (EC 4.1.2.13), hexokinase II (ATP: D-hexose 6-phosphotransferase, EC 2.7.1.1), and triosephosphate isomerase (EC 5.3.1.1) in the hypoxic culture [7].
• The glycolytic enzyme triosephosphate isomerase (TPI) catalyses the reversible conversion of dihydroxyacetone phosphate into glyceraldehyde-3-phosphate [1].

References