Disease relevance of PI15

• A novel trypsin inhibitor (P25TI) with an apparent molecular size of 25 kDa has previously been purified from the culture medium of human glioblastoma cells [1].
• P25TI mRNA was frequently expressed in human neuroblastoma and glioblastoma cell lines [1].

High impact information on PI15

• The maximal hypoglycaemic increments of MSNA were not different (HI 15 +/- 4; PI 15 +/- 2 bursts min-1 (P:NS)) Responses of plasma noradrenaline and haemodynamic parameters did not differ either [2].
• The predicted LGL1 gene product (lgl1) showed 81% homology to P25TI, a polypeptide trypsin inhibitor recently identified in human glioblastoma and neuroblastoma cells but not detected in normal human tissues [3].
• The P25TI sequence has no homology to other proteinase inhibitors, but has similarity to insect venom allergens, mammalian testis-specific proteins and plant pathogenesis-related proteins [1].
• In this study, the cDNA encoding P25TI was isolated by the polymerase chain reaction (PCR) screening system, and its complete amino acid sequence was determined [1].
• Although Northern blotting analysis failed to detect P25TI mRNA in various human tissues, PCR analysis showed its expression in the brain, placenta and lymphocytes [1].

References