Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

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Gene: CLIC5 - chloride intracellular channel 5

Homo sapiens

Synonyms: Chloride intracellular channel protein 5, CLIC5B, dJ447E21.4, FLJ90663, MST130, MSTP130

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Gagnon, L.H., Shanks, R.A., Berryman, M.

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High impact information on CLIC5

A splice variant of CLIC5, the predicted molecular mass of CLIC5B corresponds to the molecular mass of the 46-kDa CLIC immunoreactive protein in HCA-7 cells [1].
Antibodies against CLIC5B colocalized with AKAP350 at the Golgi apparatus with minor staining of the centrosomes [1].
Immunoblot analysis of extracts from placental tissues demonstrated that CLIC4 and CLIC5 are enriched in isolated placental microvilli, whereas CLIC1 is not [2].
Identification of a novel member of the chloride intracellular channel gene family (CLIC5) that associates with the actin cytoskeleton of placental microvilli [2].
Refined immunolocalization in rat and chicken vestibular hair cells showed that CLIC5 is limited to the basal region of the hair bundle, similar to the known location of radixin [3].

Anatomical context of CLIC5

In wild-type mice, CLIC5-specific immunofluorescence was detected in stereocilia of both cochlear and vestibular hair cells and also along the apical surface of Kolliker's organ during cochlear development [3].
These results suggest that CLIC5 associates with radixin in hair cell stereocilia and may help form or stabilize connections between the plasma membrane and the filamentous actin core [3].
By mass spectrometry and immunoblotting, CLIC5 was shown to be expressed at high levels in stereocilia of the chicken utricle, in an approximate 1:1 molar ratio with radixin [3].

Associations of CLIC5 with chemical compounds

AKAP350 and CLIC5B association with Golgi elements was lost following brefeldin A treatment [1].

Analytical, diagnostic and therapeutic context of CLIC5

Western blot and immunohistochemistry results confirmed the predicted absence of CLIC5 protein in tissues of jbg/jbg mutant mice [3].

References

AKAP350 at the Golgi apparatus. II. Association of AKAP350 with a novel chloride intracellular channel (CLIC) family member. Shanks, R.A., Larocca, M.C., Berryman, M., Edwards, J.C., Urushidani, T., Navarre, J., Goldenring, J.R. J. Biol. Chem. (2002)
Identification of a novel member of the chloride intracellular channel gene family (CLIC5) that associates with the actin cytoskeleton of placental microvilli. Berryman, M., Bretscher, A. Mol. Biol. Cell (2000)
The chloride intracellular channel protein CLIC5 is expressed at high levels in hair cell stereocilia and is essential for normal inner ear function. Gagnon, L.H., Longo-Guess, C.M., Berryman, M., Shin, J.B., Saylor, K.W., Yu, H., Gillespie, P.G., Johnson, K.R. J. Neurosci. (2006)