The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)



Gene Review

POU2F2  -  POU class 2 homeobox 2

Homo sapiens

Synonyms: Lymphoid-restricted immunoglobulin octamer-binding protein NF-A2, OCT2, OTF-2, OTF2, Oct-2, ...
Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.

Disease relevance of POU2F2

  • Oct-2, therefore, acts as a cell survival factor in t(14;18) lymphoma cells by directly activating the antiapoptotic gene bcl-2 [1].
  • A fragment of Oct-2 containing POUHD and an adjoining linker was expressed in Escherichia coli and characterized by three-dimensional nuclear magnetic resonance (3D-NMR) spectroscopy [2].
  • To analyze the various Oct2 isoforms, they were overexpressed in HeLa cells using recombinant vaccinia virus [3].
  • In this study, the mRNA levels of the organic ion transporters were quantified by real-time PCR in normal parts of renal tissues from seven nephrectomized patients with renal cell carcinoma, and the distributions and localization of human (h)OAT1, hOAT3, and hOCT2 proteins were investigated by immunohistochemical analyses in the human kidney [4].
  • Lower Prevalence of the OCT2 Ser270 Allele in Patients with Essential Hypertension* [5].

High impact information on POU2F2

  • Although these two elements have no sequence similarity, they are both recognized in vitro by the ubiquitous octamer transcription factor OTF-1 (reviewed refs 13 and 14) and the lymphoid-specific OTF-2 (reviewed in refs 15 and 16) [6].
  • Oct-2 also possesses a potential 'leucine zipper' domain, where four leucines are each separated by exactly seven residues [7].
  • The DNA-binding domain of Oct-2 is structurally related to the homeo box consensus and thus contains a potential helix-turn-helix sequence [7].
  • Octamer factor 2 (Oct-2, OTF-2, NF-A2) is an 'upstream' promoter factor that binds to the octamer motif (ATGCAAAT) implicated in control of immunoglobulin gene transcription in B-lymphocytes [8].
  • Many studies indicate that the cell type-specific transcription factor OTF-2 plays a central role in the lymphoid-specific transcription of immunoglobulin genes [9].

Chemical compound and disease context of POU2F2

  • These findings demonstrate that uptake of cis-platin is mediated by hOCT2 in renal proximal tubules, explaining its organ-specific toxicity [10].
  • A hypothetical three-dimensional structure of OCT2 based on a homology model that used the Escherichia coli glycerol 3-phosphate transporter as a template has been described (Zhang, X., Shirahatti, N. V., Mahadevan, D., and Wright, S. H. (2005) J. Biol. Chem. 280, 34813-34822) [11].

Biological context of POU2F2

  • The genes encoding for these three respective transcription factors have been mapped at 11p11.2 (SPI1), 11q23.1 (POU2AF1), and 19q13.2 (POU2F2); these are chromosomes recurrently affected in HL [12].
  • The functional changes of hOCT2 variants were evaluated in vitro, and those genetic polymorphisms of hOCTs were compared among different ethnic populations [13].
  • From direct DNA sequencing, 7 of 13 coding variants were nonsynonymous single-nucleotide polymorphisms (SNPs), including four variants from hOCT1 (F160L, P283L, P341L, and M408V) and three from hOCT2 (T199I, T201M, and A270S), whereas 6 were synonymous SNPs [13].
  • This conclusion is supported by the finding that transfection of HEK293 cells with cDNA encoding either hOCT1, hOCT2, or hOCT3 did not enhance specific [(14)C]agmatine accumulation compared to nontransfected cells [14].
  • In addition, human hs4 enhancer activity required Oct-2 and correlated with expression of Oct coactivator from B cells (OCA-B) [15].

Anatomical context of POU2F2

  • Co-transfection of human TBP and Oct2 expression vectors into B cells resulted in a synergistic activation of an octamer motif containing promoter [16].
  • Gene-targeting studies showed that Oct-1 and Oct-2 are largely dispensable for B-cell development and immunoglobulin production, although both Oct-2 and Bob-1 are required for a proper immune response and germinal center formation [1].
  • Furthermore, Oct-1 in combination with co-regulator Bob.1, or Oct-2 alone, could drive transcription of a heterologous thymidine kinase promoter linked to the FR in both B cells and epithelial cells [17].
  • While Oct-1 is ubiquitous, Oct-2 is predominantly expressed in B cells, in activated T cells and in nervous system [18].
  • In other cell types however, all three isoforms activate the involucrin promoter and this effect is also observed at low concentrations of Oct-2 in keratinocytes [19].

Associations of POU2F2 with chemical compounds

  • The different alternatively spliced isoforms of the Oct-2 transcription factor repress the involucrin promoter in a cell type-specific manner [19].
  • The activation required binding of OTF-1/OTF-2, and C4 could not stimulate transcription by itself, implying a synergistic interaction [20].
  • Sarkosyl inhibition studies suggest that both Oct1 and Oct2 function in vitro by stabilizing preinitiation complexes without affecting the reinitiation rate of RNA polymerase II [3].
  • The Organic Cation Transporter 2, OCT2 (SLC22A2), has been implicated in renal dopamine handling as well as in the inactivation of circulating catecholamines and is supposed to be involved in blood pressure regulation [5].
  • However, oxaliplatin showed almost no influence on the TEA uptakes in the HEK293 cells expressing hOCT1, hOCT2, and hOCT3 [21].

Physical interactions of POU2F2

  • We show that both affinity-purified OTF-1 and OTF-2 bind to the TAATGARAT sequence and that Vmw65 induces the formation of an additional complex that involves OTF-1 and that is further retarded in a band-shift gel assay [22].
  • The B-lymphocyte-specific activity of the immunoglobulin mu heavy-chain gene enhancer has been attributed to the octamer motif (ATTTGCAT) present within the enhancer that binds a B-cell-specific factor designated NF-A2/OTF-2 [23].

Regulatory relationships of POU2F2


Other interactions of POU2F2

  • We had previously shown that the ubiquitous Oct1 and the lymphoid-specific Oct2 transcription factors stimulate transcription at the level of stable preinitiation complex formation [16].
  • We show that the POU homeodomain of Oct2 and the evolutionarily conserved C-terminal core domain of TBP are both required and sufficient for the interaction [16].
  • An NMR model is obtained from Oct-2, a human B-cell specific transcription factor which participates in the regulation of immunoglobulin genes [2].
  • In transfected mammalian cells, a transcriptionally inert wild-type but not an L501P GR peptide potentiated transcriptional activation by Oct-2 100-fold above the level that could be attained in the cell by expressing Oct-2 alone [24].
  • However, the stimulatory effect was not specific to FMBP-1, BYB also enhancing the binding of mammalian transcription factors OTF2, SP1 and AP2 to their specific binding elements [25].

Analytical, diagnostic and therapeutic context of POU2F2


  1. Oct transcription factors mediate t(14;18) lymphoma cell survival by directly regulating bcl-2 expression. Heckman, C.A., Duan, H., Garcia, P.B., Boxer, L.M. Oncogene (2006) [Pubmed]
  2. Solution structure of a POU-specific homeodomain: 3D-NMR studies of human B-cell transcription factor Oct-2. Sivaraja, M., Botfield, M.C., Mueller, M., Jancso, A., Weiss, M.A. Biochemistry (1994) [Pubmed]
  3. Analysis of transcriptional stimulation by recombinant Oct proteins in a cell-free system. Annweiler, A., Zwilling, S., Hipskind, R.A., Wirth, T. J. Biol. Chem. (1993) [Pubmed]
  4. Gene expression levels and immunolocalization of organic ion transporters in the human kidney. Motohashi, H., Sakurai, Y., Saito, H., Masuda, S., Urakami, Y., Goto, M., Fukatsu, A., Ogawa, O., Inui, K. J. Am. Soc. Nephrol. (2002) [Pubmed]
  5. Lower Prevalence of the OCT2 Ser270 Allele in Patients with Essential Hypertension*. Lazar, A., Zimmermann, T., Koch, W., Gr??ndemann, D., Sch??mig, A., Kastrati, A., Sch??mig, E. Clin. Exp. Hypertens. (2006) [Pubmed]
  6. Functional cooperativity between protein molecules bound at two distinct sequence elements of the immunoglobulin heavy-chain promoter. Poellinger, L., Yoza, B.K., Roeder, R.G. Nature (1989) [Pubmed]
  7. The B-cell-specific Oct-2 protein contains POU box- and homeo box-type domains. Clerc, R.G., Corcoran, L.M., LeBowitz, J.H., Baltimore, D., Sharp, P.A. Genes Dev. (1988) [Pubmed]
  8. Oct-2 facilitates functional preinitiation complex assembly and is continuously required at the promoter for multiple rounds of transcription. Arnosti, D.N., Merino, A., Reinberg, D., Schaffner, W. EMBO J. (1993) [Pubmed]
  9. The cell type-specific octamer transcription factor OTF-2 has two domains required for the activation of transcription. Gerster, T., Balmaceda, C.G., Roeder, R.G. EMBO J. (1990) [Pubmed]
  10. Cisplatin nephrotoxicity is critically mediated via the human organic cation transporter 2. Ciarimboli, G., Ludwig, T., Lang, D., Pavenstädt, H., Koepsell, H., Piechota, H.J., Haier, J., Jaehde, U., Zisowsky, J., Schlatter, E. Am. J. Pathol. (2005) [Pubmed]
  11. Cysteine accessibility in the hydrophilic cleft of human organic cation transporter 2. Pelis, R.M., Zhang, X., Dangprapai, Y., Wright, S.H. J. Biol. Chem. (2006) [Pubmed]
  12. Alterations of loci encoding PU.1, BOB1, and OCT2 transcription regulators do not correlate with their suppressed expression in Hodgkin lymphoma. Cavazzini, F., De Wolf-Peeters, C., Wlodarska, I. Cancer Genet. Cytogenet. (2005) [Pubmed]
  13. Identification and functional characterization of genetic variants of human organic cation transporters in a korean population. Kang, H.J., Song, I.S., Shin, H.J., Kim, W.Y., Lee, C.H., Shim, J.C., Zhou, H.H., Lee, S.S., Shin, J.G. Drug Metab. Dispos. (2007) [Pubmed]
  14. Identification and pharmacological characterization of a specific agmatine transport system in human tumor cell lines. Molderings, G.J., Bruss, M., Bonisch, H., Gothert, M. Ann. N. Y. Acad. Sci. (2003) [Pubmed]
  15. NF-kappa B and Oct-2 synergize to activate the human 3' Igh hs4 enhancer in B cells. Sepulveda, M.A., Emelyanov, A.V., Birshtein, B.K. J. Immunol. (2004) [Pubmed]
  16. The POU domains of the Oct1 and Oct2 transcription factors mediate specific interaction with TBP. Zwilling, S., Annweiler, A., Wirth, T. Nucleic Acids Res. (1994) [Pubmed]
  17. Functional interaction of Oct transcription factors with the family of repeats in Epstein-Barr virus oriP. Almqvist, J., Zou, J., Linderson, Y., Borestrom, C., Altiok, E., Zetterberg, H., Rymo, L., Pettersson, S., Ernberg, I. J. Gen. Virol. (2005) [Pubmed]
  18. Human Oct-1L isoform has tissue-specific expression pattern similar to Oct-2. Luchina, N.N., Krivega, I.V., Pankratova, E.V. Immunol. Lett. (2003) [Pubmed]
  19. The different alternatively spliced isoforms of the Oct-2 transcription factor repress the involucrin promoter in a cell type-specific manner. Chapman, C.M., Latchman, D.S. Mol. Biol. Rep. (1998) [Pubmed]
  20. Identification of novel ubiquitous and cell type-specific factors that specifically recognize immunoglobulin heavy chain and kappa light chain promoters. Franke, S., Scholz, G., Scheidereit, C. J. Biol. Chem. (1994) [Pubmed]
  21. Cisplatin and Oxaliplatin, but Not Carboplatin and Nedaplatin, Are Substrates for Human Organic Cation Transporters (SLC22A1-3 and Multidrug and Toxin Extrusion Family). Yonezawa, A., Masuda, S., Yokoo, S., Katsura, T., Inui, K. J. Pharmacol. Exp. Ther. (2006) [Pubmed]
  22. A herpesvirus trans-activating protein interacts with transcription factor OTF-1 and other cellular proteins. Gerster, T., Roeder, R.G. Proc. Natl. Acad. Sci. U.S.A. (1988) [Pubmed]
  23. Complex regulation of the immunoglobulin mu heavy-chain gene enhancer: microB, a new determinant of enhancer function. Nelsen, B., Kadesch, T., Sen, R. Mol. Cell. Biol. (1990) [Pubmed]
  24. Recruitment of octamer transcription factors to DNA by glucocorticoid receptor. Préfontaine, G.G., Lemieux, M.E., Giffin, W., Schild-Poulter, C., Pope, L., LaCasse, E., Walker, P., Haché, R.J. Mol. Cell. Biol. (1998) [Pubmed]
  25. Bombyx Y-box protein BYB facilitates specific DNA interaction of various DNA binding proteins independently of the cold shock domain. Takiya, S., Nishita, Y., Ishikawa, S., Ohno, K., Tamura, T.A., Suzuki, Y. J. Biochem. (2004) [Pubmed]
  26. 3-Hydroxy-3-methylglutaryl coenzyme A reductase inhibitors increase the binding activity and nuclear level of Oct-1 in mononuclear cells. Ortego, M., Hernández, A.G., Bustos, C., Blanco-Colio, L.M., Hernández-Presa, M.A., Tuñón, J., Egido, J. Eur. J. Pharmacol. (2002) [Pubmed]
  27. The B cell-specific nuclear factor OTF-2 positively regulates transcription of the human class II transplantation gene, DRA. Zeleznik-Le, N.J., Itoh-Lindstrom, Y., Clarke, J.B., Moore, T.L., Ting, J.P. J. Biol. Chem. (1992) [Pubmed]
WikiGenes - Universities