Disease relevance of Mllt1

• In particular, leukemias with the t(11;19)(q23;p13.3) translocation express HRX-ENL fusion proteins and display features which suggest the malignant transformation of myeloid and/or lymphoid progenitor(s) [1].
• Immortalized cells or enriched primary hematopoietic stem cells transduced with HRX-ENL induced myeloid leukemias in syngeneic and SCID recipients [1].

High impact information on Mllt1

• Immortalization and leukemic transformation of a myelomonocytic precursor by retrovirally transduced HRX-ENL [1].
• ABI1 was identified as an ENL binding protein by a yeast two-hybrid screen [2].
• We have mapped Mllt1 to mouse chromosome 17 band E2 using FISH analysis [3].
• The leukemia-associated gene Mllt1/ENL: characterization of a murine homolog and demonstration of an essential role in embryonic development [3].
• The murine homolog Mllt1 exhibits 86% amino acid sequence identity with the human gene and is broadly expressed in murine tissues and cell lines, with the exception of liver and myeloid cell lines [3].

Anatomical context of Mllt1

• To characterize directly the potential transforming effects of HRX-ENL on primitive hematopoietic precursors, the fusion cDNA was transduced by retroviral gene transfer into cell populations enriched in hematopoietic stem cells [1].

Analytical, diagnostic and therapeutic context of Mllt1

• The interaction of ENL and ABI1 could be verified in vitro by far-Western blot assays and GST-pulldown studies as well as in vivo by co-immunoprecipitation experiments [2].

References