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SRSF7  -  serine/arginine-rich splicing factor 7

Homo sapiens

Synonyms: 9G8, AAG3, HSSG1, RBM37, SFRS7, ...
 
 
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Disease relevance of SFRS7

  • We show that a recombinant 9G8 protein, expressed using a baculovirus vector and excluding other SR factors, rescues the splicing activity of a 9G8-depleted nuclear extract and an S100 cytoplasmic fraction [1].
  • These results indicate that 9G8 plays a key role in regulation of exon 10 splicing and imply a pathogenic role in neurodegenerative diseases [2].
 

Psychiatry related information on SFRS7

  • SR protein 9G8 modulates splicing of tau exon 10 via its proximal downstream intron, a clustering region for frontotemporal dementia mutations [2].
 

High impact information on SFRS7

  • Antibodies to SRp20 or 9G8 eliminate RNA binding and significantly inhibit the export of RNAs carrying the element from oocyte nuclei [3].
  • Our observation that SRp20 and 9G8 can be UV cross-linked to polyadenylated RNA in both the nucleus and cytoplasm of HeLa cells suggests a more general role for these SR proteins in mRNA export [3].
  • Coupling of transcription with alternative splicing: RNA pol II promoters modulate SF2/ASF and 9G8 effects on an exonic splicing enhancer [4].
  • We present the solution structures of the free 9G8 and SRp20 RNA recognition motifs (RRMs) and of SRp20 RRM in complex with the RNA sequence 5'CAUC3'. The SRp20-RNA structure reveals that although all 4 nt are contacted by the RRM, only the 5' cytosine is primarily recognized in a specific way [5].
  • This might explain the numerous consensus sequences found by SELEX (systematic evolution of ligands by exponential enrichment) for the RRM of 9G8 and SRp20 [5].
 

Biological context of SFRS7

  • The identification of the 9G8 factor enlarges the essential family of SR splicing factors, whose members have also been proposed to play key roles in alternative splicing [1].
  • Compared with the other SR splicing factors, 9G8 presents some specific sequence features because it contains an RRSRSXSX consensus sequence repeated six times in the SR domain, and a CCHC motif in its median region, similar to the zinc knuckle found in the SLU7 splicing factor in yeast [1].
  • We have located the human 9G8 gene in the p22-21 region of chromosome 2 [6].
  • Using a two-hybrid interactive screen, the splicing protein 9G8 was identified as an in vivo partner for CDK11(p110) [7].
  • Finally, 9G8 is a phosphoprotein in vivo and is a substrate for CDK11(p110) phosphorylation in vitro [7].
 

Associations of SFRS7 with chemical compounds

  • Characterization and cloning of the human splicing factor 9G8: a novel 35 kDa factor of the serine/arginine protein family [1].
  • The isolation and characterization of cDNA clones indicate that 9G8 is a novel member of the serine/arginine (SR) splicing factor family because it includes an N-terminal RNA binding domain (RBD) and a C-terminal SR domain [1].
  • SRZ proteins are similar to human 9G8 splicing factor because they contain a zinc knuckle, precipitate with 65% ammonium sulfate, and cross-react with the 9G8 monoclonal antibody [8].
  • Here we show that a 35-kDa member of the SR protein family, 9G8, can activate the splicing of alpha-tropomyosin exon 2 [9].
 

Physical interactions of SFRS7

  • In addition, we have found that PIR1 interacted with splicing factors 9G8 and SRp30C, possibly through an RNA intermediate during a yeast two-hybrid screen [10].
 

Other interactions of SFRS7

References

  1. Characterization and cloning of the human splicing factor 9G8: a novel 35 kDa factor of the serine/arginine protein family. Cavaloc, Y., Popielarz, M., Fuchs, J.P., Gattoni, R., Stévenin, J. EMBO J. (1994) [Pubmed]
  2. SR protein 9G8 modulates splicing of tau exon 10 via its proximal downstream intron, a clustering region for frontotemporal dementia mutations. Gao, L., Wang, J., Wang, Y., Andreadis, A. Mol. Cell. Neurosci. (2007) [Pubmed]
  3. Splicing factors SRp20 and 9G8 promote the nucleocytoplasmic export of mRNA. Huang, Y., Steitz, J.A. Mol. Cell (2001) [Pubmed]
  4. Coupling of transcription with alternative splicing: RNA pol II promoters modulate SF2/ASF and 9G8 effects on an exonic splicing enhancer. Cramer, P., Cáceres, J.F., Cazalla, D., Kadener, S., Muro, A.F., Baralle, F.E., Kornblihtt, A.R. Mol. Cell (1999) [Pubmed]
  5. Molecular basis of RNA recognition and TAP binding by the SR proteins SRp20 and 9G8. Hargous, Y., Hautbergue, G.M., Tintaru, A.M., Skrisovska, L., Golovanov, A.P., Stevenin, J., Lian, L.Y., Wilson, S.A., Allain, F.H. EMBO J. (2006) [Pubmed]
  6. The gene encoding human splicing factor 9G8. Structure, chromosomal localization, and expression of alternatively processed transcripts. Popielarz, M., Cavaloc, Y., Mattei, M.G., Gattoni, R., Stévenin, J. J. Biol. Chem. (1995) [Pubmed]
  7. CDK11 complexes promote pre-mRNA splicing. Hu, D., Mayeda, A., Trembley, J.H., Lahti, J.M., Kidd, V.J. J. Biol. Chem. (2003) [Pubmed]
  8. The plant U1 small nuclear ribonucleoprotein particle 70K protein interacts with two novel serine/arginine-rich proteins. Golovkin, M., Reddy, A.S. Plant Cell (1998) [Pubmed]
  9. Activation of {alpha}-Tropomyosin Exon 2 Is Regulated by the SR Protein 9G8 and Heterogeneous Nuclear Ribonucleoproteins H and F. Crawford, J.B., Patton, J.G. Mol. Cell. Biol. (2006) [Pubmed]
  10. PIR1, a novel phosphatase that exhibits high affinity to RNA . ribonucleoprotein complexes. Yuan, Y., Li, D.M., Sun, H. J. Biol. Chem. (1998) [Pubmed]
  11. Differential effects of the SR proteins 9G8, SC35, ASF/SF2, and SRp40 on the utilization of the A1 to A5 splicing sites of HIV-1 RNA. Ropers, D., Ayadi, L., Gattoni, R., Jacquenet, S., Damier, L., Branlant, C., Stévenin, J. J. Biol. Chem. (2004) [Pubmed]
  12. Alternative splicing of intron 3 of the serine/arginine-rich protein 9G8 gene. Identification of flanking exonic splicing enhancers and involvement of 9G8 as a trans-acting factor. Lejeune, F., Cavaloc, Y., Stevenin, J. J. Biol. Chem. (2001) [Pubmed]
 
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