Disease relevance of COX1

• CONCLUSION.: These findings may implicate COX-1 as a suitable target for the prevention or treatment of ovarian cancer [1].

High impact information on COX1

• One of the sequenced products was identified as subunit I of cytochrome-c oxidase (COX-1), an enzyme which is central to energy metabolism and particularly relevant for developing nervous systems [2].
• These data not only show that the developing ciliary margin of the chick retina has high energy requirements, but also indicate that COX-1 could play essential roles in developing cells and in stem cells of the eye periphery [2].
• Considering that the induction of COX 1 and COX2 are inhibited by ketoprofen, and that these enzymes are located in the stromal compartment of the CAM, we propose that its antiangiogenic effect may occur via inhibition of the two COX isoforms [3].
• In a series of trials, young (3 to 5 wk of age) cockerels infected with Eimeria acervulina, a duodenal parasite, received oral treatments with indomethacin (IM), an inhibitor of both COX-1 and COX-2, or nimesulide (NIM), a specific inhibitor of COX-2 [4].
• Here we report the RT-PCR cloning of a chicken cDNA that encodes the amphipathic membrane binding region and parts of the dimerization and catalytic domains of COX-1-like enzyme [5].

Biological context of COX1

• This transcript was not detectable during chick embryogenesis and, as is the case for mammalian COX-1, was not induced above background by mitogen stimulation [5].

Anatomical context of COX1

• Furthermore, whereas COX-1 in mammals is broadly expressed in tissues as a constitutive enzyme, the mRNA detected by our clone in chicken was almost absent in tissues and embryo fibroblasts (CEF) [5].

Analytical, diagnostic and therapeutic context of COX1

• Immunohistochemistry using antibodies for COX-1 and COX-2 was used to localize protein expression of each isoform in a subset of tumor (n = 5) and normal samples (n = 6) [1].
• Quantitative real-time PCR with Sybr Green was used to quantify the mRNA expression of COX-1 and COX-2, using 18S expression as an internal control for COX normalization [1].

References