Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

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Gene: BUD3 - Bud3p

Saccharomyces cerevisiae

Synonyms: Bud site selection protein 3, Bud site selection protein BUD3, YCL014W/YCL013W/YCL012W, YCL14W/YCL13W/YCL12W

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Chant, J., Sanders, S.L., Halme, A., Neuwald, A.F., Freedman, T., et al.

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High impact information on BUD3

Here we report a physical association of promoter and terminator regions of the yeast BUD3 and SEN1 genes [1].
Genetic analysis indicates that AXL2 falls into the same epistasis group as BUD3 [2].
BUD3 is expressed close to the onset of mitosis [3].
Two other bud site selection genes, RSR1, encoding a small GTPase, and BUD3, were also required for efficient cell fusion [4].
Mutations in BUD3, BUD4, and AXL1 cause a and alpha cells to exhibit the bipolar pattern, indicating that these genes are necessary to specify the axial budding pattern (Chant, J., and I. Herskowitz. 1991. Cell. 65:1203-1212; Fujita, A., C. Oka, Y. Arikawa, T. Katagi, A. Tonouchi, S. Kuhara, and Y. Misumi. 1994. Nature (Lond.). 372:567-570) [5].

Biological context of BUD3

These observations suggest that Bud4p and Bud3p cooperate to recognize a spatial landmark (the neck filaments) during mitosis and support the hypothesis that they subsequently become a landmark for establishing the axial budding pattern in G1 [5].
Taken together, the data suggest that Bud3p and the neck filaments are linked in a cycle in which each controls the position of the other's assembly: Bud3p assembles onto the neck filaments in one cell cycle to mark the site for axial budding (including assembly of the new ring of neck filaments) in the next cell cycle [6].
In the axial budding pattern, cells polarize and divide adjacent to the previous site of cell separation, in response to a cell-division remnant, which includes Bud3p, Bud4p and septin proteins [7].
These include: an obscure subfamily of globins in the roundworm Caenorhabditis elegans ; two new superfamilies of metallohydrolases; a lipoyl/biotin swinging arm domain in bacterial membrane fusion proteins; and a DH domain in the yeast Bud3 and Fus2 proteins [8].
Mutants defective in bipolar budding were identified by virtue of their inability to grow as pseudohyphae in a haploid bud3 background [9].

Anatomical context of BUD3

Clusters of Bud10p at the mother-bud neck formed in response to Bud3p (and possibly to an extracellular cue, such as a component of the cell wall), might facilitate the docking of downstream components that direct polarization of the cytoskeleton [7].

Co-localisations of BUD3

Clb2 co-localizes with the bud site selection protein Bud3 [10].

Other interactions of BUD3

Upon shift of a temperature-sensitive cdc12 mutant to restrictive temperature, localization of both Bud3p and the neck filament-associated proteins is rapidly lost [6].
The Bud3p structure at the neck persists until cytokinesis, when it splits to yield a single ring of Bud3p marking the division site on each of the two progeny cells [6].

Analytical, diagnostic and therapeutic context of BUD3

However, immunofluorescence localization of Bud3p has revealed that it assembles in an apparent double ring encircling the mother-bud neck shortly after the mitotic spindle forms [6].

References

A role for the CPF 3'-end processing machinery in RNAP II-dependent gene looping. Ansari, A., Hampsey, M. Genes Dev. (2005)
Selection of axial growth sites in yeast requires Axl2p, a novel plasma membrane glycoprotein. Roemer, T., Madden, K., Chang, J., Snyder, M. Genes Dev. (1996)
Cell cycle programs of gene expression control morphogenetic protein localization. Lord, M., Yang, M.C., Mischke, M., Chant, J. J. Cell Biol. (2000)
A role for a protease in morphogenic responses during yeast cell fusion. Elia, L., Marsh, L. J. Cell Biol. (1998)
The BUD4 protein of yeast, required for axial budding, is localized to the mother/BUD neck in a cell cycle-dependent manner. Sanders, S.L., Herskowitz, I. J. Cell Biol. (1996)
Role of Bud3p in producing the axial budding pattern of yeast. Chant, J., Mischke, M., Mitchell, E., Herskowitz, I., Pringle, J.R. J. Cell Biol. (1995)
Bud10p directs axial cell polarization in budding yeast and resembles a transmembrane receptor. Halme, A., Michelitch, M., Mitchell, E.L., Chant, J. Curr. Biol. (1996)
Extracting protein alignment models from the sequence database. Neuwald, A.F., Liu, J.S., Lipman, D.J., Lawrence, C.E. Nucleic Acids Res. (1997)
Mutational and hyperexpression-induced disruption of bipolar budding in yeast. Freedman, T., Porter, A., Haarer, B. Microbiology (Reading, Engl.) (2000)
Differential cellular localization among mitotic cyclins from Saccharomyces cerevisiae: a new role for the axial budding protein Bud3 in targeting Clb2 to the mother-bud neck. Bailly, E., Cabantous, S., Sondaz, D., Bernadac, A., Simon, M.N. J. Cell. Sci. (2003)