High impact information on CLB4

• Thus, Clb4/Cdc28 prevents Kar9 loading on the mother bound pole [1].
• Three of these (CLB1, CLB2, and CLB4) encode proteins strongly homologous to G2-specific B-type cyclins [2].
• Cdk1-Clb4 controls the interaction of astral microtubule plus ends with subdomains of the daughter cell cortex [3].
• Clb5 also has a function, along with Clb3 and Clb4, in the formation of mitotic spindles [4].
• Because the cell cycle machinery participates in Saccharomyces cerevisiae filamentous growth, we characterized in detail the two C. albicans B-type cyclins, CLB2 and CLB4, to better understand the molecular mechanisms that underlie the C. albicans morphogenic switch [5].

Biological context of CLB4

• CLB1 and CLB4 seem to be much more important for meiosis than for mitosis and may play some special role in meiosis II [6].
• In contrast, SBF dissociates from the CLN2 promoter when transcription is repressed during G2 and M phases, suggesting that Clb1-Clb4 repress SBF activity by inhibiting its DNA-binding activity [7].
• Coincidentally with reaching the bud cortex in S phase, Cdc28-Clb4 facilitates the dissociation of the microtubule bud tip interaction and their capture by the bud neck [3].

Anatomical context of CLB4

• Clb4/Cdc28 kinase phosphorylates Kar9 and accumulates on the pole destined to the mother cell [1].

Other interactions of CLB4

• We have cloned four cyclin-B homologs from Saccharomyces cerevisiae, CLB1-CLB4, using the polymerase chain reaction and low stringency hybridization approaches [8].

References