High impact information on COS7

• The LRP-PrP interaction was confirmed in vivo in insect (Sf9) and mammalian cells (COS-7) [1].
• Glutathione S-transferase pull-down assays showed the selective interaction of alphaSNAP with G alpha12 in COS-7 as well as in human umbilical vein endothelial cells [2].
• AGS4/G18.1b was also complexed with G(i)alpha(3) in COS7 cell lysates following cell transfection [3].
• Gel filtration chromatography of COS7 cell cytosol showed that both recombinant and endogenous hVps35, hVps29, and hVps26 coelute as a large complex ( approximately 220-440 kDa) [4].
• Distribution patterns of various MAP1A domains as well as their interactions with microtubules were monitored in a non-neuronal COS7 and a neuronal Neuro2A cells [5].

Biological context of COS7

• Possible interactions between ICA512/IA-2 and components of the cytoskeleton were not supported by studies on staining of fixed yeast cells with phalloidin-Texas Red. With transfected mammalian cell lines COS-7 and NIH3T3, expression of ICA512/IA-2 likewise induced growth arrest, with some of the morphological features of apoptosis [6].

Anatomical context of COS7

• We directly demonstrated that plasmin cleaves these same sites in full-length membrane-embedded receptor expressed in yeast and COS7 fibroblasts [7].
• Processing of mutated proinsulin with tetrabasic cleavage sites to bioactive insulin in the non-endocrine cell line, COS-7 [8].
• When cDNA for cauliflower cyt b5 was introduced into mammalian (COS-7) and yeast cells as well as into onion cells, the expressed cytochrome was localized both in the ER and mitochondria in those cells [9].

Associations of COS7 with chemical compounds

• Met-Enkephalin was expressed in four nonneuroendocrine cell lines: COS-7, C2C12, Ltk-, and NIH3T3 [10].

Analytical, diagnostic and therapeutic context of COS7

• Immunocytochemistry with AGS4/G18.1b-specific antisera indicated a predominant nonhomogeneous, extranuclear distribution within the cell following expression in COS7 or Chinese hamster ovary cells [3].

1. The human 37-kDa laminin receptor precursor interacts with the prion protein in eukaryotic cells. Rieger, R., Edenhofer, F., Lasmézas, C.I., Weiss, S. Nat. Med. (1997)
2. G alpha12 interaction with alphaSNAP induces VE-cadherin localization at endothelial junctions and regulates barrier function. Andreeva, A.V., Kutuzov, M.A., Vaiskunaite, R., Profirovic, J., Meigs, T.E., Predescu, S., Malik, A.B., Voyno-Yasenetskaya, T. J. Biol. Chem. (2005)
3. Identification and characterization of AGS4: a protein containing three G-protein regulatory motifs that regulate the activation state of Gialpha. Cao, X., Cismowski, M.J., Sato, M., Blumer, J.B., Lanier, S.M. J. Biol. Chem. (2004)
4. Human orthologs of yeast vacuolar protein sorting proteins Vps26, 29, and 35: assembly into multimeric complexes. Haft, C.R., de la Luz Sierra, M., Bafford, R., Lesniak, M.A., Barr, V.A., Taylor, S.I. Mol. Biol. Cell (2000)
5. The functional cooperation of MAP1A heavy chain and light chain 2 in the binding of microtubules. Chien, C.L., Lu, K.S., Lin, Y.S., Hsieh, C.J., Hirokawa, N. Exp. Cell Res. (2005)
6. Expression of protein tyrosine phosphatase-like molecule ICA512/IA-2 induces growth arrest in yeast cells and transfected mammalian cell lines. Papakonstantinou, T., Myers, M.A., Jois, J., Roucou, X., Prescott, M., Rowley, M.J., Mackay, I.R. J. Autoimmun. (2001)
7. Plasmin desensitization of the PAR1 thrombin receptor: kinetics, sites of truncation, and implications for thrombolytic therapy. Kuliopulos, A., Covic, L., Seeley, S.K., Sheridan, P.J., Helin, J., Costello, C.E. Biochemistry (1999)
8. Processing of mutated proinsulin with tetrabasic cleavage sites to bioactive insulin in the non-endocrine cell line, COS-7. Yanagita, M., Nakayama, K., Takeuchi, T. FEBS Lett. (1992)
9. Dual subcellular distribution of cytochrome b5 in plant, cauliflower, cells. Zhao, J., Onduka, T., Kinoshita, J.Y., Honsho, M., Kinoshita, T., Shimazaki, K., Ito, A. J. Biochem. (2003)
10. Production of bioactive enkephalin from the nonendocrine cell lines COS-7, NIH3T3, Ltk-, and C2C12. Takahashi, K., Fujita, T., Takeuchi, T. Peptides (1995)