Disease relevance of CLB3

• Isolation and characterisation of a mutation in the PMR1 gene encoding a Golgi membrane ATPase, which causes hypersensitivity to over-expression of Clb3 in Saccharomyces cerevisiae [1].

High impact information on CLB3

• The CLB2 and CLB3 cyclins also accumulate in the cim mutants [2].
• Clb5 also has a function, along with Clb3 and Clb4, in the formation of mitotic spindles [3].
• During the mitotic cell cycle, Clb3 and Clb4 were expressed and induced a kinase activity in association with p34cdc28 from early S phase up to mitosis [4].
• Most of cyclin Clb3 is degraded independently of Cdh1 [5].
• The pmr1-1 strain proved to be defective in glycosylation, and the defects in glycosylation were exacerbated by high levels of Clb3 [1].

Biological context of CLB3

• The pattern of expression of Clb3 and Clb4 suggests a possible role in the regulation of DNA replication as well as mitosis [4].
• The fact that Clb3 and Clb4 were not synthesized earlier, in S phase, suggests that these cyclins, which probably have a role in S phase during the mitotic cell cycle, are not implicated in premeiotic S phase [4].
• On induction of Clb3 expression in the pmr1-1 strain, the cells arrested at anaphase with an elongated daughter bud [1].

References