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REG1  -  Reg1p

Saccharomyces cerevisiae S288c

 
 
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High impact information on REG1

  • In contrast, expression of LexA-Reg1p containing mutations at phenylalanine in the putative PP-1C-binding site motif (K/R)(X)(I/V)XF was unable to rescue Hxk2p dephosphorylation in intact yeast or restore Hxk2p phosphatase activity [1].
  • Protein phosphatase 1 (Glc7p) and its binding protein Reg1p are essential for the regulation of glucose repression pathways in Saccharomyces cerevisiae [1].
  • Consistent with increased phosphorylation of Hxk2p in response to REG1 deletion, fractionation of yeast extracts by anion-exchange chromatography identified Hxk2p phosphatase activity in wild-type strains that was selectively lost in the reg1Delta mutant [1].
  • Here we show that Reg1 interacts with the Snf1 catalytic domain in the two-hybrid system [2].
  • In Saccharomyces cerevisiae, the protein phosphatase type 1 (PP1)-binding protein Reg1 is required to maintain complete repression of ADH2 expression during growth on glucose [3].
 

Biological context of REG1

  • Mutants with a deletion of reg1 display a mild slow-growth defect, while reg2 mutants exhibit a wild-type phenotype [4].
  • The HEX2 gene encodes a protein of 114137 Da, deduced from its DNA sequence [5].
  • Deletion of REG1 in a wild-type background led to overaccumulation of glycogen as well as slow growth and an enlarged cell size [6].
  • The REG1 gene encodes a regulatory subunit of the type-1 protein phosphatase (PP1) G1c7 in Saccharomyces cerevisiae, which directs the catalytic subunit to substrates involved in glucose repression [7].
  • These lesions proved to be in the same gene, SRN1, identified previously in a search for second-site suppressors of mutations that affect the removal of intervening sequences from pre-mRNAs [8].
 

Anatomical context of REG1

  • The glc7-T152K mutant strain exhibited a reduced Reg1p binding along with defects in FBPase degradation and Vid vesicle trafficking to the vacuole [9].
 

Associations of REG1 with chemical compounds

  • These results indicate that REG1 has a unique role in the glucose repression pathway but acts together with REG2 to regulate some as yet uncharacterized function important for growth [4].
  • This indicates that REG1 can act independently of phosphorylation at serine 230 [10].
  • The REG1 gene product is required for repression of INO1 and other inositol-sensitive upstream activating sequence-containing genes of yeast [11].
  • The hex2 mutants have pleiotropic defects in the regulation of glucose-repressible enzymes, hexokinase PII synthesis and maltose uptake [Entian, K.-D. & Zimmermann, F.K. (1980) Mol. Gen. Genet. 177, 345-350] [5].
  • Cells lacking the Reg1 protein, a regulatory subunit for the Glc7 phosphatase, showed constitutive phosphorylation of Snf1 threonine 210 [12].
 

Physical interactions of REG1

  • Functional analysis of the yeast Glc7-binding protein Reg1 identifies a protein phosphatase type 1-binding motif as essential for repression of ADH2 expression [3].
 

Enzymatic interactions of REG1

  • Mutants of gene HEX1 had a reduced hexose phosphorylating activity on all media whereas those of gene HEX2 had elevated levels but only in glucose grown cells [13].
 

Other interactions of REG1

  • The phosphorylation state of Hxk2p and Hxk2p phosphatase activity was restored to wild-type levels in the reg1Delta mutant by expression of a LexA-Reg1p fusion protein [1].
  • Overexpression of HEX2 resulted in a 70% reduction of GAL1 expression under induced growth conditions [5].
  • In order to identify physiological substrates for the Glc7p-Reg1p complex, we examined the effects of deletion of the REG1 gene on the yeast phosphoproteome [1].
  • Protein phosphatase type-1 regulatory subunits Reg1p and Reg2p act as signal transducers in the glucose-induced inactivation of maltose permease in Saccharomyces cerevisiae [7].
  • Interestingly, SRN1 is not a negative regulator of RNA1 at the transcriptional, translational, or protein stability level [8].
 

Analytical, diagnostic and therapeutic context of REG1

  • Flow cytometry measurements showed that G1 arrest of the hex2 mutant under such conditions was incomplete [14].

References

  1. Reg1p targets protein phosphatase 1 to dephosphorylate hexokinase II in Saccharomyces cerevisiae: characterizing the effects of a phosphatase subunit on the yeast proteome. Alms, G.R., Sanz, P., Carlson, M., Haystead, T.A. EMBO J. (1999) [Pubmed]
  2. Glucose-regulated interaction of a regulatory subunit of protein phosphatase 1 with the Snf1 protein kinase in Saccharomyces cerevisiae. Ludin, K., Jiang, R., Carlson, M. Proc. Natl. Acad. Sci. U.S.A. (1998) [Pubmed]
  3. Functional analysis of the yeast Glc7-binding protein Reg1 identifies a protein phosphatase type 1-binding motif as essential for repression of ADH2 expression. Dombek, K.M., Voronkova, V., Raney, A., Young, E.T. Mol. Cell. Biol. (1999) [Pubmed]
  4. The REG2 gene of Saccharomyces cerevisiae encodes a type 1 protein phosphatase-binding protein that functions with Reg1p and the Snf1 protein kinase to regulate growth. Frederick, D.L., Tatchell, K. Mol. Cell. Biol. (1996) [Pubmed]
  5. Characterization of Hex2 protein, a negative regulatory element necessary for glucose repression in yeast. Niederacher, D., Entian, K.D. Eur. J. Biochem. (1991) [Pubmed]
  6. Genetic interactions between REG1/HEX2 and GLC7, the gene encoding the protein phosphatase type 1 catalytic subunit in Saccharomyces cerevisiae. Huang, D., Chun, K.T., Goebl, M.G., Roach, P.J. Genetics (1996) [Pubmed]
  7. Protein phosphatase type-1 regulatory subunits Reg1p and Reg2p act as signal transducers in the glucose-induced inactivation of maltose permease in Saccharomyces cerevisiae. Jiang, H., Tatchell, K., Liu, S., Michels, C.A. Mol. Gen. Genet. (2000) [Pubmed]
  8. SRN1, a yeast gene involved in RNA processing, is identical to HEX2/REG1, a negative regulator in glucose repression. Tung, K.S., Norbeck, L.L., Nolan, S.L., Atkinson, N.S., Hopper, A.K. Mol. Cell. Biol. (1992) [Pubmed]
  9. The type 1 phosphatase Reg1p-Glc7p is required for the glucose-induced degradation of fructose-1,6-bisphosphatase in the vacuole. Cui, D.Y., Brown, C.R., Chiang, H.L. J. Biol. Chem. (2004) [Pubmed]
  10. ADH2 expression is repressed by REG1 independently of mutations that alter the phosphorylation of the yeast transcription factor ADR1. Dombek, K.M., Camier, S., Young, E.T. Mol. Cell. Biol. (1993) [Pubmed]
  11. The REG1 gene product is required for repression of INO1 and other inositol-sensitive upstream activating sequence-containing genes of yeast. Ouyang, Q., Ruiz-Noriega, M., Henry, S.A. Genetics (1999) [Pubmed]
  12. Regulation of Snf1 kinase. Activation requires phosphorylation of threonine 210 by an upstream kinase as well as a distinct step mediated by the Snf4 subunit. McCartney, R.R., Schmidt, M.C. J. Biol. Chem. (2001) [Pubmed]
  13. Glycolytic enzymes and intermediates in carbon catabolite repression mutants of Saccharomyces cerevisiae. Entian, K.D., Zimmermann, F.K. Mol. Gen. Genet. (1980) [Pubmed]
  14. Constitutive glucose-induced activation of the Ras-cAMP pathway and aberrant stationary-phase entry on a glucose-containing medium in the Saccharomyces cerevisiae glucose-repression mutant hex2. Dumortier, F., Argüelles, J.C., Thevelein, J.M. Microbiology (Reading, Engl.) (1995) [Pubmed]
 
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