Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

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Gene: GIC2 - Gic2p

Saccharomyces cerevisiae

Synonyms: D9740.18, GTPase-interacting component 2, Protein of unknown function involved in initiation of budding and cellular polarization, interacts with Cdc42p via the Cdc42/Rac-interactive binding (CRIB) domain, YDR309C

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Jaquenoud, M., Jaquenoud, M., Hsiung, Y.G., Brown, J.L., Chen, G.C., et al.

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High impact information on GIC2

Novel Cdc42-binding proteins Gic1 and Gic2 control cell polarity in yeast [1].
Gic1p and Gic2p colocalize with Cdc42p as cell polarity is established during the cell cycle and during mating in response to pheromones [1].
The Cdc42p effector Gic2p is targeted for ubiquitin-dependent degradation by the SCFGrr1 complex [2].
Gic2p is expressed in a cell cycle-dependent manner and rapidly disappears shortly after bud emergence concomitant with the activation of the G1 cyclin-dependent kinase Cdc28p-Clnp [2].
Finally, our data indicate that degradation of Gic2p may be part of a mechanism which restricts cytoskeletal polarization in the G1 phase of the cell cycle [2].

Biological context of GIC2

Gic1 and Gic2 are two Cdc42/Rac interactive binding (CRIB) domain-containing effectors of Cdc42-GTPase that promote polarized cell growth in S. cerevisiae [3].
The Gic1 protein, together with its structural homolog Gic2, are required for cell size and shape control, bud site selection, bud emergence, actin cytoskeletal organization, mitotic spindle orientation/positioning, and mating projection formation in response to mating pheromone [4].
Phosphorylation of several C-terminal sites of Gic2p served as part of the recognition signal for ubiquitination [2].
The same mutations resulted in the stabilization of Cln2 and Gic2 and also in a spectrum of phenotypes characteristic of inactivation of GRR1, including hyperpolarization and enhancement of pseudohyphal growth [5].

Associations of GIC2 with chemical compounds

In addition to its role in degradation of Gic2 and the CDK targets Cln1 and Cln2, Grr1 is also required for induction of glucose- and amino acid-regulated genes [6].

Physical interactions of GIC2

We identified a conserved domain in Gic2p which was necessary for its polarization function but dispensable for binding to Cdc42p-GTP and its localization to the site of polarization [7].

Other interactions of GIC2

Thus, Gic1p and Gic2p define a novel class of Cdc42p targets that are specifically required for cytoskeletal polarization in vivo [1].
RESULTS: Here we show that a rsr1 gic1 gic2 mutant fails to initiate budding, resulting in unbudded, large, and multinucleated cells [8].
Expression of a mutant Gic2p harboring a single-amino-acid substitution in this domain (Gic2p(W23A)) interfered with polarized growth in a dominant-negative manner and prevented recruitment of Bni1p and Bud6p to the incipient bud site [7].
Isolation and characterization of HRT1 using a genetic screen for mutants unable to degrade Gic2p in saccharomyces cerevisiae [9].
Here we have shown that the rapid disappearance of Gic2p results from ubiquitin-dependent proteolysis [2].

References

Novel Cdc42-binding proteins Gic1 and Gic2 control cell polarity in yeast. Brown, J.L., Jaquenoud, M., Gulli, M.P., Chant, J., Peter, M. Genes Dev. (1997)
The Cdc42p effector Gic2p is targeted for ubiquitin-dependent degradation by the SCFGrr1 complex. Jaquenoud, M., Gulli, M.P., Peter, K., Peter, M. EMBO J. (1998)
Four Novel Suppressors of gic1 gic2 and Their Roles in Cytokinesis and Polarized Cell Growth in Saccharomyces cerevisiae. Gandhi, M., Goode, B.L., Chan, C.S. Genetics (2006)
The Cdc42 GTPase-associated proteins Gic1 and Gic2 are required for polarized cell growth in Saccharomyces cerevisiae. Chen, G.C., Kim, Y.J., Chan, C.S. Genes Dev. (1997)
F-box protein Grr1 interacts with phosphorylated targets via the cationic surface of its leucine-rich repeat. Hsiung, Y.G., Chang, H.C., Pellequer, J.L., La Valle, R., Lanker, S., Wittenberg, C. Mol. Cell. Biol. (2001)
Regulation and recognition of SCFGrr1 targets in the glucose and amino acid signaling pathways. Spielewoy, N., Flick, K., Kalashnikova, T.I., Walker, J.R., Wittenberg, C. Mol. Cell. Biol. (2004)
Gic2p may link activated Cdc42p to components involved in actin polarization, including Bni1p and Bud6p (Aip3p). Jaquenoud, M., Peter, M. Mol. Cell. Biol. (2000)
The upstream regulator, Rsr1p, and downstream effectors, Gic1p and Gic2p, of the Cdc42p small GTPase coordinately regulate initiation of budding in Saccharomyces cerevisiae. Kawasaki, R., Fujimura-Kamada, K., Toi, H., Kato, H., Tanaka, K. Genes Cells (2003)
Isolation and characterization of HRT1 using a genetic screen for mutants unable to degrade Gic2p in saccharomyces cerevisiae. Blondel, M., Galan, J.M., Peter, M. Genetics (2000)