High impact information on CYT1

• Qcr9p and Cyt1p are two subunits of the respiratory complex bc1 and their relative roles in the assembly of other respiratory complexes have been investigated [1].
• We have previously shown that mutations in the TM domain of Cyt1p can partially compensate for the absence of Oxa1p [1].
• Regulation of the yeast CYT1 gene encoding cytochrome c1 by HAP1 and HAP2/3/4 [2].
• Unlike cytochrome c, which is encoded by two genes, CYC1 and CYC7, c1 is encoded by a single gene, CYT1 [2].
• A systematic screen for dominant-negative mutations of the CYT1 gene, which encodes cytochrome c(1), revealed seven mutants after testing approximately 10(4) Saccharomyces cerevisiae strains transformed with a library of mutagenized multicopy plasmids [3].

Biological context of CYT1

• Functional analyses after the separate introduction of point mutations into both elements reveal no role for the latter protein and only a minor role for Cpf1p in the regulated expression of CYT1/lacZ chimaeric proteins [4].
• DNA sequence analysis revealed multiple nucleotide substitutions with six of the seven altered Cyt1p having a common R166G replacement, either by itself or accompanied with other amino acid replacements [3].

Other interactions of CYT1

• Among 26 fusions shown to be regulated by Hap2p only CYT1 was known to be regulated by this activator [5].

References