Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

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Gene Review:

RPA43 - Rpa43p

Saccharomyces cerevisiae S288c

Synonyms: A43, DNA-directed DNA-dependent RNA polymerase 36 kDa polypeptide, DNA-directed RNA polymerase I subunit RPA43, O6271, RRN12, ...

Riva, M. et al., Cavanaugh, A.H. et al., Imazawa, Y. et al., Meka, H. et al., Thuriaux, P. et al., et al.

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Disease relevance of RPA43

In three cases (lambda A-43, lambda A-40, and lambda A-34.5), an antigenic protein was expressed in E. coli with the same molecular weight as the corresponding subunit [1].

High impact information on RPA43

A protruding protein density named stalk was found to contain the RNA pol I-specific subunits A43 and A14 [2].
Rrn3 produced in Sf9 cells but not in bacteria interacts with rpa43 in vitro, and such interaction is dependent upon the phosphorylation state of Rrn3 [3].
RPA43 mapped next to RPA190, encoding the largest subunit of polymerase I. These genes are divergently transcribed and may thus share upstream regulatory elements ensuring their co-regulation [4].
One meiotic segregant had a hybrid RNA polymerase A with five of the polymorphic polypeptides (A49, A43, A19, A14.5, and A14) coming from S. douglasii and two (A40 and A34.5) from S. cerevisiae [5].
We show here that Schizosaccharomyces pombe rpa21(+) encodes a protein of apparent molecular mass 21 kD which shows 36% identity to the A43 subunit of pol I in S. cerevisiae, and that rpa21(+) is essential for cell growth [6].

Associations of RPA43 with chemical compounds

5. Without the two Saccharomyces species, S. cerevisiae and S. douglasii, 7 of 13 polypeptides of enzyme A(A49, A43, A40, A34.5, A19, A14.5, and A14) differ slightly in molecular weight and can be resolved by electrophoresis on polyacrylamide gel [5].
Cycloheximide inhibits the phosphorylation of Rrn3 and causes its dissociation from RNA polymerase I. Rrn3 interacts with the rpa43 subunit of RNA polymerase I, and treatment with cycloheximide inhibits the formation of a Rrn3.rpa43 complex in vivo [3].

Physical interactions of RPA43

To shed light on the function of the heterodimer, we performed gel mobility shift assays and showed that the A14/A43 heterodimer binds single-stranded RNA in a similar way to the archaeal E/F complex [7].

Analytical, diagnostic and therapeutic context of RPA43

Consistently, immunoelectron microscopy data showed that A43 and Rrn3 co-localize within the Pol I-Rrn3 complex [8].

References

Isolation of structural genes for yeast RNA polymerases by immunological screening. Riva, M., Memet, S., Micouin, J.Y., Huet, J., Treich, I., Dassa, J., Young, R., Buhler, J.M., Sentenac, A., Fromageot, P. Proc. Natl. Acad. Sci. U.S.A. (1986) [Pubmed]
Localization of the yeast RNA polymerase I-specific subunits. Bischler, N., Brino, L., Carles, C., Riva, M., Tschochner, H., Mallouh, V., Schultz, P. EMBO J. (2002) [Pubmed]
Rrn3 phosphorylation is a regulatory checkpoint for ribosome biogenesis. Cavanaugh, A.H., Hirschler-Laszkiewicz, I., Hu, Q., Dundr, M., Smink, T., Misteli, T., Rothblum, L.I. J. Biol. Chem. (2002) [Pubmed]
Gene RPA43 in Saccharomyces cerevisiae encodes an essential subunit of RNA polymerase I. Thuriaux, P., Mariotte, S., Buhler, J.M., Sentenac, A., Vu, L., Lee, B.S., Nomura, M. J. Biol. Chem. (1995) [Pubmed]
Natural variation in yeast RNA polymerase A. Formation of a mosaic RNA polymerase A in a meiotic segregant from an interspecific hybrid. Riva, M., Buhler, J.M., Sentenac, A., Fromageot, P., Hawthorne, D.C. J. Biol. Chem. (1982) [Pubmed]
The fission yeast RPA21 subunit of RNA polymerase I: an evolutionarily conserved subunit interacting with ribosomal DNA (rDNA) transcription factor Rrn3p for recruitment to rDNA promoter. Imazawa, Y., Hisatake, K., Nakagawa, K., Muramatsu, M., Nogi, Y. Genes Genet. Syst. (2002) [Pubmed]
Structural and functional homology between the RNAP(I) subunits A14/A43 and the archaeal RNAP subunits E/F. Meka, H., Daoust, G., Arnvig, K.B., Werner, F., Brick, P., Onesti, S. Nucleic Acids Res. (2003) [Pubmed]
The recruitment of RNA polymerase I on rDNA is mediated by the interaction of the A43 subunit with Rrn3. Peyroche, G., Milkereit, P., Bischler, N., Tschochner, H., Schultz, P., Sentenac, A., Carles, C., Riva, M. EMBO J. (2000) [Pubmed]

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