The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

Links

 

Gene Review

MAPKAPK5  -  mitogen-activated protein kinase-activated...

Homo sapiens

Synonyms: MAP kinase-activated protein kinase 5, MAPK-activated protein kinase 5, MAPKAP kinase 5, MAPKAP-K5, MAPKAPK-5, ...
 
 
Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.
 

High impact information on MAPKAPK5

  • They show that the p38-regulated/activated protein kinase (PRAK) induces senescence downstream of oncogenic Ras by directly phosphorylating and activating the tumor-suppressor protein p53 [1].
  • An in-gel kinase assay demonstrated that PRAK is a major stress-activated kinase that can phosphorylate small heat shock protein, suggesting a potential role for PRAK in mediating stress-induced HSP27 phosphorylation in vivo [2].
  • PRAK activity was regulated by p38alpha and p38beta both in vitro and in vivo and Thr182 was shown to be the regulatory phosphorylation site [2].
  • We have identified and cloned a novel serine/ threonine kinase, p38-regulated/activated protein kinase (PRAK) [2].
  • PRAK was found to be expressed in all human tissues and cell lines examined [2].
 

Biological context of MAPKAPK5

  • Thus, the subcellular distribution of PRAK is determined by multiple factors including its own NES and NLS, docking interactions between PRAK and docking proteins, phosphorylation of PRAK, and cellular activation status [3].
  • A sequence analysis of PRAK shows that PRAK contains both a putative nuclear export sequence (NES) and a nuclear localization sequence (NLS) [3].
 

Anatomical context of MAPKAPK5

  • In HeLa cells, PRAK was activated in response to cellular stress and proinflammatory cytokines [2].
 

Associations of MAPKAPK5 with chemical compounds

  • Phosphorylation by MSK1 induced an increase in Vmax and a decrease in Km for 6-(R)-5,6,7,8-tetrahydrobiopterin (BH4), while these kinetic parameters were unaffected as a result of phosphorylation by PRAK [4].
  • Epigallocatechin 3-gallate, one of the main polyphenolic constituents of tea, inhibited two of the 28 protein kinases in the panel, dual-specificity, tyrosine-phosphorylated and regulated kinase 1A (DYRK1A; IC(50)=0.33 microM) and p38-regulated/activated kinase (PRAK; IC(50)=1.0 microM) [5].
 

Enzymatic interactions of MAPKAPK5

  • Activated PRAK in turn phosphorylated small heat shock protein 27 (HSP27) at the physiologically relevant sites [2].
 

Regulatory relationships of MAPKAPK5

  • Recombinant human tyrosine hydroxylase (hTH1) was found to be phosphorylated by mitogen and stress-activated protein kinase 1 (MSK1) at Ser40 and by p38 regulated/activated kinase (PRAK) on Ser19 [4].

References

  1. Oncogene-induced senescence pathways weave an intricate tapestry. Yaswen, P., Campisi, J. Cell (2007) [Pubmed]
  2. PRAK, a novel protein kinase regulated by the p38 MAP kinase. New, L., Jiang, Y., Zhao, M., Liu, K., Zhu, W., Flood, L.J., Kato, Y., Parry, G.C., Han, J. EMBO J. (1998) [Pubmed]
  3. Regulation of PRAK subcellular location by p38 MAP kinases. New, L., Jiang, Y., Han, J. Mol. Biol. Cell (2003) [Pubmed]
  4. Regulation of tyrosine hydroxylase by stress-activated protein kinases. Toska, K., Kleppe, R., Armstrong, C.G., Morrice, N.A., Cohen, P., Haavik, J. J. Neurochem. (2002) [Pubmed]
  5. The specificities of protein kinase inhibitors: an update. Bain, J., McLauchlan, H., Elliott, M., Cohen, P. Biochem. J. (2003) [Pubmed]
 
WikiGenes - Universities