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GAL83  -  Gal83p

Saccharomyces cerevisiae S288c

Synonyms: Glucose repression protein GAL83, Protein SPM1, SNF1 protein kinase subunit beta-3, SPM1, YER027C
 
 
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High impact information on GAL83

  • We show that Gal83 directs Snf1 to the nucleus in a glucose-regulated manner [1].
  • Green fluorescent protein fusions to Gal83, Sip1, and Sip2 show different patterns of localization to the nucleus, vacuole, and/or cytoplasm [1].
  • Gal83 interacts with Sip4 in two-hybrid assays in vivo, and bacterially expressed proteins bind in vitro [2].
  • Alteration of Gal83 at residues that are important for glycogen binding of AMPK beta1 abolished glycogen binding in vitro and caused diverse phenotypes in vivo [3].
  • Various Snf1/Gal83-dependent processes were upregulated, including glycogen accumulation, expression of RNAs encoding glycogen synthase, haploid invasive growth, the transcriptional activator function of Sip4, and activation of the carbon source-responsive promoter element [3].
 

Biological context of GAL83

 

Anatomical context of GAL83

  • We previously reported the identification of Mkh1, a MEK kinase in Schizosaccharomyces pombe that is required for cell wall integrity, and we presented genetic evidence that Pmk1/Spm1, a MAP kinase, functions downstream from Mkh1 in the same pathway [7].
 

Associations of GAL83 with chemical compounds

  • We have isolated GAL83, one of these genes required for glucose repression [8].
  • Addition of glutathione to the medium inhibited nuclear accumulation of GFP-Yap1 in response to carbon stress but did not affect the relocalization of Gal83 or Mig1 [9].
 

Physical interactions of GAL83

  • We propose that members of the Sip1/Sip2/Gal83 family confer specificity to the kinase complex in its interactions with target proteins [2].
 

Regulatory relationships of GAL83

  • Mutations in the gal83 glycogen-binding domain activate the snf1/gal83 kinase pathway by a glycogen-independent mechanism [3].
 

Other interactions of GAL83

  • Tests of the ability of each suppressor to cross-suppress mutations in the other glucose repression genes revealed two groups of mutually cross-suppressed genes: (1) REG1, GAL82 and GAL83 and (2) GRR1 [10].
  • It was shown that StubSNF1 interacted with the GAL83 ortholog of potato, StubGAL83, and complemented the Delta snf1 mutation [11].
  • Moreover, it suppressed Delta snf4 and Delta sip1,Delta sip2,Delta gal83 deficiencies [11].
  • The GAL82 and GAL83 mutations produced partial resistance of galactokinase to glucose repression only when one or both of these mutations were combined with a GAL81 or a gal80 mutation [6].
  • Saccharomyces cerevisiae encodes three beta-subunit genes, SIP1, SIP2 and GAL83 [12].

References

  1. Subcellular localization of the Snf1 kinase is regulated by specific beta subunits and a novel glucose signaling mechanism. Vincent, O., Townley, R., Kuchin, S., Carlson, M. Genes Dev. (2001) [Pubmed]
  2. Gal83 mediates the interaction of the Snf1 kinase complex with the transcription activator Sip4. Vincent, O., Carlson, M. EMBO J. (1999) [Pubmed]
  3. Mutations in the gal83 glycogen-binding domain activate the snf1/gal83 kinase pathway by a glycogen-independent mechanism. Wiatrowski, H.A., Van Denderen, B.J., Berkey, C.D., Kemp, B.E., Stapleton, D., Carlson, M. Mol. Cell. Biol. (2004) [Pubmed]
  4. FOG1 and FOG2 genes, required for the transcriptional activation of glucose-repressible genes of Kluyveromyces lactis, are homologous to GAL83 and SNF1 of saccharomyces cerevisiae. Goffrini, P., Ficarelli, A., Donnini, C., Lodi, T., Puglisi, P.P., Ferrero, I. Curr. Genet. (1996) [Pubmed]
  5. SIP1 is a catabolite repression-specific negative regulator of GAL gene expression. Mylin, L.M., Bushman, V.L., Long, R.M., Yu, X., Lebo, C.M., Blank, T.E., Hopper, J.E. Genetics (1994) [Pubmed]
  6. Isolation and characterization of dominant mutations resistant to carbon catabolite repression of galactokinase synthesis in Saccharomyces cerevisiae. Matsumoto, K., Toh-e, A., Oshima, Y. Mol. Cell. Biol. (1981) [Pubmed]
  7. Skh1, the MEK component of the mkh1 signaling pathway in Schizosaccharomyces pombe. Loewith, R., Hubberstey, A., Young, D. J. Cell. Sci. (2000) [Pubmed]
  8. Genetic and molecular characterization of GAL83: its interaction and similarities with other genes involved in glucose repression in Saccharomyces cerevisiae. Erickson, J.R., Johnston, M. Genetics (1993) [Pubmed]
  9. Yap1 accumulates in the nucleus in response to carbon stress in Saccharomyces cerevisiae. Wiatrowski, H.A., Carlson, M. Eukaryotic Cell (2003) [Pubmed]
  10. Suppressors reveal two classes of glucose repression genes in the yeast Saccharomyces cerevisiae. Erickson, J.R., Johnston, M. Genetics (1994) [Pubmed]
  11. Functional diversity of potato SNF1-related kinases tested in Saccharomyces cerevisiae. Lovas, A., Sós-Hegedus, A., Bimbó, A., Bánfalvi, Z. Gene (2003) [Pubmed]
  12. beta-subunits of Snf1 kinase are required for kinase function and substrate definition. Schmidt, M.C., McCartney, R.R. EMBO J. (2000) [Pubmed]
 
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