Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

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Gene: FCY2 - Fcy2p

Saccharomyces cerevisiae

Synonyms: Cytosine/purine transport protein FCY2, Fluorocytosine resistance protein 2, PCP FCY2, Purine-cytosine permease FCY2, YER056C

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Paluszynski, J.P., Grandier-Vazeille, X., Weber, E., Wagner, R., Pinson, B., et al.

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Disease relevance of FCY2

By applying a highly sensitive assay, a low-level but dose-dependent toxicity of 5-FC in fcy2 mutants was detected, whereas cells deficient in the cytosine deaminase (encoded by FCY1), which is essential for intracellular conversion of 5-FC to 5-fluorouracil, display strong dose-independent resistance [1].

High impact information on FCY2

The FCY2 gene was mapped on the right arm of chromosome V close to the HIS1 gene [2].
Expression of the FCY2 gene product requires a functional secretory pathway and is reduced in mnn9, a mutant strain deficient in outer chain glycosylation [2].
BRA7 is allelic to FCY2, the gene encoding the purine cytosine permease and BRA9 is ADE12, the gene encoding adenylosuccinate synthetase [3].
The FCY2 gene of Saccharomyces cerevisiae encodes a purine-cytosine permease (PCP) that mediates the active transport of purines and cytosine [4].
A genome-wide search for cytosine permease homologues identified two uncharacterized candidate genes, designated FCY21 and FCY22, both of which exhibit highest similarity to FCY2 [1].

Biological context of FCY2

The corresponding plasmids were introduced into a S. cerevisiae strain carrying a chromosomal deletion at the FCY2 locus [5].
A synthetic peptide, selected in the predicted N-terminal amino-acid sequence of the purine cytosine permease (gene FCY2), linked to albumins proved a remarkably good immunogen in rabbits [6].

Anatomical context of FCY2

The purine/cytosine permease, encoded by the FCY2 gene, is a carrier located in the plasma membrane of the yeast Saccharomyces cerevisiae [7].

Associations of FCY2 with chemical compounds

5-FC uptake in S. cerevisiae was considered to be mediated by the FCY2-encoded cytosine/adenine permease [1].

Analytical, diagnostic and therapeutic context of FCY2

In ELISA, sera reacted with the synthetic peptide and with specific proteins of plasma-membrane-enriched fractions of mutant Saccharomyces cerevisiae pAB strains (amplified FCY2 gene) with high titers and high avidity [6].

References

Various cytosine/adenine permease homologues are involved in the toxicity of 5-fluorocytosine in Saccharomyces cerevisiae. Paluszynski, J.P., Klassen, R., Rohe, M., Meinhardt, F. Yeast (2006)
The purine-cytosine permease gene of Saccharomyces cerevisiae: primary structure and deduced protein sequence of the FCY2 gene product. Weber, E., Rodriguez, C., Chevallier, M.R., Jund, R. Mol. Microbiol. (1990)
The isolation and characterization of Saccharomyces cerevisiae mutants that constitutively express purine biosynthetic genes. Guetsova, M.L., Lecoq, K., Daignan-Fornier, B. Genetics (1997)
New plasmid system to select for Saccharomyces cerevisiae purine-cytosine permease affinity mutants. Wagner, R., Straub, M.L., Souciet, J.L., Potier, S., de Montigny, J. J. Bacteriol. (2001)
In vivo and in vitro studies of the purine-cytosine permease of Saccharomyces cerevisiae. Functional analysis of a mutant with an altered apparent transport constant of uptake. Brèthes, D., Chirio, M.C., Napias, C., Chevallier, M.R., Lavie, J.L., Chevallier, J. Eur. J. Biochem. (1992)
Detection of purine cytosine permease of S. cerevisiae: use of antibodies against a synthetic peptide corresponding to a predicted sequence in the N-terminal domain of the protein. Grandier-Vazeille, X., Neaud, V., Geoffre, S. Biochem. Biophys. Res. Commun. (1993)
In vivo phosphorylation of the purine/cytosine permease from the plasma membrane of the yeast Saccharomyces cerevisiae. Pinson, B., Pillois, X., Brèthes, D., Chevallier, J., Napias, C. Eur. J. Biochem. (1996)