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Gene Review

RUVBL1  -  RuvB-like AAA ATPase 1

Homo sapiens

Synonyms: 49 kDa TATA box-binding protein-interacting protein, 49 kDa TBP-interacting protein, 54 kDa erythrocyte cytosolic protein, ECP-54, ECP54, ...
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Disease relevance of RUVBL1


High impact information on RUVBL1


Biological context of RUVBL1

  • The TIP49b/RUVBL2 open reading frame encodes a protein of 463 amino acids, showing 43% identity with the RUVBL1 protein [8].
  • Analysis of the nucleotide sequences revealed that ECP-51 and ECP-54 are homologous (44.2% amino acid identity) and contain ATP-binding sites [9].
  • TIP48/TIP49 complex formation resulted in synergistic increase in ATPase activity but ATP hydrolysis was not stimulated in the presence of single-stranded, double-stranded or four-way junction DNA and no DNA helicase or branch migration activity could be detected [7].
  • These results are consistent with our previous results from tissue distribution analysis for of TIP49 proteins [3].
  • Chromosome mapping and expression of human tip49 family genes [3].

Anatomical context of RUVBL1

  • TIP49b/RUVBL2, like RUVBL1, was expressed ubiquitously in all human tissues examined and more strongly in testis [8].
  • Confocal immunoflourescence microscopy reveals that RUVBL1/TIP49a was present not only in the nucleus, as expected, but was also concentrated at the centrosome and at the mitotic spindle in colocalization with tubulin [10].
  • Identification and characterization of the ubiquitously occurring nuclear matrix protein NMP 238 [11].

Associations of RUVBL1 with chemical compounds

  • We recovered and identified RUVBL1/TIP49a as a tubulin-binding protein from Triton X-100 lysates of U937 promonocytic cells by protein affinity chromatography and tryptic peptide microsequencing [10].
  • Three-dimensional reconstruction at 20 A resolution revealed that the TIP48/TIP49 complex consisted of two stacked hexameric rings with C6 symmetry [7].
  • Moreover, glycerol gradient analysis demonstrated that TIP49 is present in a macromolecular complex in nuclear extracts [2].
  • Using histidine-tagged TBP for affinity-purification of TBP-bound proteins, we isolated a 49-kD protein termed TBP-interacting protein 49 (TIP49) from rat liver nuclear extracts [12].
  • RUVBL1/TIP49a/Pontin52 is a recently identified multi-functional protein with 2 ATP binding (WALKER) sites, which is essential for cell proliferation [10].

Physical interactions of RUVBL1


Other interactions of RUVBL1

  • The interaction of ECP-51 and ECP-54 with the stomatin peptide and the localization to the nucleus and cytoplasm suggest an additional function for these proteins as chaperone components [9].
  • Here we show that the regulation of the expression of this enzyme in a colon cancer cell line, and in patients, is associated with overexpression of the Wnt pathway-associated proteins, Pontin52/TIP49a and LEF-1 [1].
  • RUVBL1 and PSA expression was further confirmed by real-time quantitative PCR, whose results paralleled the microarray data [13].
  • TIP49, homologous to the bacterial DNA helicase RuvB, acts as an autoantigen in human [2].
  • TBP-interacting protein 49 (TIP49) was originally identified as a TBP-binding protein, and two related proteins are encoded by individual genes, tip49a and b [3].

Analytical, diagnostic and therapeutic context of RUVBL1

  • Immunoprecipitation analysis of the cell extracts indicated that TIP49 and TBP were present in an identical complex [12].
  • Immunofluorescence analysis using anti-TIP49 antibody showed a typical dot-shaped nuclear staining pattern, suggesting that TIP49 is included in a macromolecular structure in the nucleus and may participate in nuclear events such as transcription and recombination [2].
  • Consistent with the notion that tip49 family genes are essential for cell growth, Northern blot analysis demonstrated that both genes are expressed ubiquitously in human tissues [3].
  • Expression, purification, crystallization and preliminary X-ray analysis of the human RuvB-like protein RuvBL1 [14].


  1. Regulation of COX-2 transcription in a colon cancer cell line by Pontin52/TIP49a. Carlson, M.L., Wilson, E.T., Prescott, S.M. Mol. Cancer (2003) [Pubmed]
  2. TIP49, homologous to the bacterial DNA helicase RuvB, acts as an autoantigen in human. Makino, Y., Mimori, T., Koike, C., Kanemaki, M., Kurokawa, Y., Inoue, S., Kishimoto, T., Tamura, T. Biochem. Biophys. Res. Commun. (1998) [Pubmed]
  3. Chromosome mapping and expression of human tip49 family genes. Makino, Y., Kanemaki, M., Koga, A., Osano, K., Matsu-Ura, T., Kurokawa, Y., Kishimoto, T., Tamura, T. DNA Seq. (2000) [Pubmed]
  4. TIP49 regulates beta-catenin-mediated neoplastic transformation and T-cell factor target gene induction via effects on chromatin remodeling. Feng, Y., Lee, N., Fearon, E.R. Cancer Res. (2003) [Pubmed]
  5. TIP49b, a new RuvB-like DNA helicase, is included in a complex together with another RuvB-like DNA helicase, TIP49a. Kanemaki, M., Kurokawa, Y., Matsu-ura, T., Makino, Y., Masani, A., Okazaki, K., Morishita, T., Tamura, T.A. J. Biol. Chem. (1999) [Pubmed]
  6. TIP49, but not TRRAP, modulates c-Myc and E2F1 dependent apoptosis. Dugan, K.A., Wood, M.A., Cole, M.D. Oncogene (2002) [Pubmed]
  7. Dodecameric Structure and ATPase Activity of the Human TIP48/TIP49 Complex. Puri, T., Wendler, P., Sigala, B., Saibil, H., Tsaneva, I.R. J. Mol. Biol. (2007) [Pubmed]
  8. Human TIP49b/RUVBL2 gene: genomic structure, expression pattern, physical link to the human CGB/LHB gene cluster on chromosome 19q13.3. Parfait, B., Giovangrandi, Y., Asheuer, M., Laurendeau, I., Olivi, M., Vodovar, N., Vidaud, D., Vidaud, M., Bièche, I. Ann. Genet. (2000) [Pubmed]
  9. Isolation, molecular characterization, and tissue-specific expression of ECP-51 and ECP-54 (TIP49), two homologous, interacting erythroid cytosolic proteins. Salzer, U., Kubicek, M., Prohaska, R. Biochim. Biophys. Acta (1999) [Pubmed]
  10. The ATP-dependent helicase RUVBL1/TIP49a associates with tubulin during mitosis. Gartner, W., Rossbacher, J., Zierhut, B., Daneva, T., Base, W., Weissel, M., Waldhäusl, W., Pasternack, M.S., Wagner, L. Cell Motil. Cytoskeleton (2003) [Pubmed]
  11. Identification and characterization of the ubiquitously occurring nuclear matrix protein NMP 238. Holzmann, K., Gerner, C., Korosec, T., Pöltl, A., Grimm, R., Sauermann, G. Biochem. Biophys. Res. Commun. (1998) [Pubmed]
  12. Molecular cloning of a rat 49-kDa TBP-interacting protein (TIP49) that is highly homologous to the bacterial RuvB. Kanemaki, M., Makino, Y., Yoshida, T., Kishimoto, T., Koga, A., Yamamoto, K., Yamamoto, M., Moncollin, V., Egly, J.M., Muramatsu, M., Tamura, T. Biochem. Biophys. Res. Commun. (1997) [Pubmed]
  13. Microarray analysis of gene-expression profiles in diffuse large B-cell lymphoma: identification of genes related to disease progression. Nishiu, M., Yanagawa, R., Nakatsuka, S., Yao, M., Tsunoda, T., Nakamura, Y., Aozasa, K. Jpn. J. Cancer Res. (2002) [Pubmed]
  14. Expression, purification, crystallization and preliminary X-ray analysis of the human RuvB-like protein RuvBL1. Gorynia, S., Matias, P.M., Gonçalves, S., Coelho, R., Lopes, G., Thomaz, M., Huber, M., Haendler, B., Donner, P., Carrondo, M.A. Acta Crystallograph. Sect. F Struct. Biol. Cryst. Commun. (2006) [Pubmed]
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