Disease relevance of gadC

• Southern hybridization revealed that both the gadC and hdeAB loci are absent in Salmonella [1].
• The most potent E. coli acid resistance system utilizes two isoforms of glutamate decarboxylase encoded by gadA and gadB and a putative glutamate:gamma-aminobutyric acid antiporter encoded by gadC [2].

High impact information on gadC

• The gadC gene, located downstream of gadB, has been proposed to encode a putative antiporter implicated in the export of gamma-aminobutyrate, the glutamic acid decarboxylation product [3].
• The structural genes gadA, gadB, and gadC encode two glutamate decarboxylase isoforms and a glutamate/gamma-aminobutyrate (GABA) antiporter, respectively [4].

Biological context of gadC

• Here, we describe the nucleotide sequence and functional analysis of two genes transcribed from this promoter, gadC and gadB [5].
• We confirm that gadA, gadB, and gadC are also all dependent upon sigma(s) for their expression in S. flexneri. -10 sequences similar to the sigma(s)-10 consensus sequence were identified by primer extension in the upstream promoters of all three genes and the transcriptional start points were identical in both E. coli and S. flexneri [6].

Associations of gadC with chemical compounds

• In the present work, we provide in vivo evidence that gadC is co-transcribed with gadB and that the functional glutamic acid-dependent system requires the activities of both GadA/B and GadC [3].

Other interactions of gadC

• This compound is exported via the protein product of the gadC gene, which is cotranscribed with gadB [7].

Analytical, diagnostic and therapeutic context of gadC

• An expression cassette consisting of the positive-regulator gene gadR, the chloride-inducible promoter Pgad, and the translation initiation signals of gadC was amplified by PCR [8].

References