The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 
Gene Review

crr  -  glucose-specific enzyme IIA component of PTS

Escherichia coli str. K-12 substr. MG1655

Synonyms: ECK2412, JW2410, gsr, iex, tgs, ...
 
 
Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.
 

Disease relevance of crr

  • Analysis of the ptsH-ptsI-crr region in Escherichia coli K-12: evidence for the existence of a single transcriptional unit [1].
  • Phosphotransferase-mediated regulation of carbohydrate utilisation in Escherichia coli K12: identification of the products of genes on the specialised transducing phages lambda iex (crr) and lambda gsr (tgs) [2].
  • Unique dicistronic operon (ptsI-crr) in Mycoplasma capricolum encoding enzyme I and the glucose-specific enzyme IIA of the phosphoenolpyruvate:sugar phosphotransferase system: cloning, sequencing, promoter analysis, and protein characterization [3].
  • Association of the prophage P1ban protein with the dnaB protein of Escherichia coli. Overproduction of ban protein by a P1bac crr mutant [4].
 

High impact information on crr

  • The cloned fragment also complemented cysA mutations but did not contain a functional pts operon which is closely linked to the crr gene and codes for two enzymes of the PTS [5].
  • Expression of crr plasmids in a maxicell system yielded two proteins which reacted with specific anti-serum against IIIGlc [5].
  • A 9.6-kb segment of the S. typhimurium chromosome containing the crr gene was cloned in pAT153 [5].
  • Disruption of the crr gene or overproduction of LacY also eliminated the glucose effect [6].
  • The crr promoters (P2) within ptsI may be negatively regulated by CRP.cAMP [7].
 

Chemical compound and disease context of crr

  • The B. subtilis IIIGlc domain can replace IIIGlc in E. coli crr mutants in supporting growth on glucose and transport of methyl alpha-glucoside [8].
 

Biological context of crr

  • Its membrane-bound subunit, IICB(Glc), is encoded by the gene ptsG; its soluble domain, IIA(Glc), is encoded by crr, which is a member of the pts operon [9].
  • P1 transduction and PCR mapping and DNA sequence analysis showed that pdxK was adjacent to the crr sugar transport gene (53.95 min) [10].
  • The short crr transcripts were initiated inside the ptsI open reading frame at points which were identified by S1 mapping [11].
  • These three genes are organized in a complex operon in which the major part of expression of the distal gene, crr, is initiated from a promoter region within ptsI [12].
  • One encompassed the three cistrons, a second one the ptsH gene and part of the ptsI gene, and the third one only the distal gene crr [11].
 

Associations of crr with chemical compounds

 

Other interactions of crr

  • The gamma delta transposon studies also suggest that crr is transcribed from an independent promoter located within the ptsI gene [18].
  • For example, the glucose transport genes (ptsHI, ptsG, crr) in both acetate and glycerol media were down-regulated, and the ppc, glycolytic, and biosynthetic genes in acetate were also down-regulated because of the reduced fluxes [19].
  • In glucose minimal medium a PTS- strain of Escherichia coli [delta (ptsH ptsI crr)] could grow slowly (doubling time, d = 10 h) [20].
  • S. typhimurium crr mutants are deficient in enzyme III glucose, which is a component of the glucose transport system, and a regulator of adenylate cyclase [15].
 

Analytical, diagnostic and therapeutic context of crr

  • Molecular cloning, sequencing, and expression of the crr gene: the structural gene for IIIGlc of the bacterial PEP:glucose phosphotransferase system [5].
  • Northern blot analysis indicated that ptsI and crr constitute a dicistronic operon that includes an independent promoter for the crr gene [3].

References

  1. Analysis of the ptsH-ptsI-crr region in Escherichia coli K-12: evidence for the existence of a single transcriptional unit. De Reuse, H., Huttner, E., Danchin, A. Gene (1984) [Pubmed]
  2. Phosphotransferase-mediated regulation of carbohydrate utilisation in Escherichia coli K12: identification of the products of genes on the specialised transducing phages lambda iex (crr) and lambda gsr (tgs). Britton, P., Murfitt, D., Parra, F., Jones-Mortimer, M.C., Kornberg, H.L. EMBO J. (1982) [Pubmed]
  3. Unique dicistronic operon (ptsI-crr) in Mycoplasma capricolum encoding enzyme I and the glucose-specific enzyme IIA of the phosphoenolpyruvate:sugar phosphotransferase system: cloning, sequencing, promoter analysis, and protein characterization. Zhu, P.P., Reizer, J., Peterkofsky, A. Protein Sci. (1994) [Pubmed]
  4. Association of the prophage P1ban protein with the dnaB protein of Escherichia coli. Overproduction of ban protein by a P1bac crr mutant. Edelbluth, C., Lanka, E., von der Hude, W., Mikolajczyk, M., Schuster, H. Eur. J. Biochem. (1979) [Pubmed]
  5. Molecular cloning, sequencing, and expression of the crr gene: the structural gene for IIIGlc of the bacterial PEP:glucose phosphotransferase system. Nelson, S.O., Schuitema, A.R., Benne, R., van der Ploeg, L.H., Plijter, J.S., Aan, F., Postma, P.W. EMBO J. (1984) [Pubmed]
  6. cAMP receptor protein-cAMP plays a crucial role in glucose-lactose diauxie by activating the major glucose transporter gene in Escherichia coli. Kimata, K., Takahashi, H., Inada, T., Postma, P., Aiba, H. Proc. Natl. Acad. Sci. U.S.A. (1997) [Pubmed]
  7. Evidence for two promoters upstream of the pts operon: regulation by the cAMP receptor protein regulatory complex. Fox, D.K., Presper, K.A., Adhya, S., Roseman, S., Garges, S. Proc. Natl. Acad. Sci. U.S.A. (1992) [Pubmed]
  8. The glucose permease of the phosphotransferase system of Bacillus subtilis: evidence for IIGlc and IIIGlc domains. Gonzy-Tréboul, G., de Waard, J.H., Zagorec, M., Postma, P.W. Mol. Microbiol. (1991) [Pubmed]
  9. Glucose transporter mutants of Escherichia coli K-12 with changes in substrate recognition of IICB(Glc) and induction behavior of the ptsG gene. Zeppenfeld, T., Larisch, C., Lengeler, J.W., Jahreis, K. J. Bacteriol. (2000) [Pubmed]
  10. Identification of the pdxK gene that encodes pyridoxine (vitamin B6) kinase in Escherichia coli K-12. Yang, Y., Zhao, G., Winkler, M.E. FEMS Microbiol. Lett. (1996) [Pubmed]
  11. The ptsH, ptsI, and crr genes of the Escherichia coli phosphoenolpyruvate-dependent phosphotransferase system: a complex operon with several modes of transcription. De Reuse, H., Danchin, A. J. Bacteriol. (1988) [Pubmed]
  12. Positive regulation of the pts operon of Escherichia coli: genetic evidence for a signal transduction mechanism. De Reuse, H., Danchin, A. J. Bacteriol. (1991) [Pubmed]
  13. Molecular population genetics of Escherichia coli: DNA sequence diversity at the celC, crr, and gutB loci of natural isolates. Hall, B.G., Sharp, P.M. Mol. Biol. Evol. (1992) [Pubmed]
  14. Detecting selective sweeps in naturally occurring Escherichia coli. Guttman, D.S., Dykhuizen, D.E. Genetics (1994) [Pubmed]
  15. Evidence against direct involvement of cyclic GMP or cyclic AMP in bacterial chemotactic signaling. Tribhuwan, R.C., Johnson, M.S., Taylor, B.L. J. Bacteriol. (1986) [Pubmed]
  16. Mutational analysis of the enzyme IIIGlc of the phosphoenolpyruvate phosphotransferase system in Escherichia coli. Zeng, G.Q., De Reuse, H., Danchin, A. Res. Microbiol. (1992) [Pubmed]
  17. Sugar transport. The crr mutation: its effect on repression of enzyme synthesis. Saier, M.H., Roseman, S. J. Biol. Chem. (1976) [Pubmed]
  18. Sugar transport by the bacterial phosphotransferase system. Molecular cloning and structural analysis of the Escherichia coli ptsH, ptsI, and crr genes. Saffen, D.W., Presper, K.A., Doering, T.L., Roseman, S. J. Biol. Chem. (1987) [Pubmed]
  19. Gene expression profiling by DNA microarrays and metabolic fluxes in Escherichia coli. Oh, M.K., Liao, J.C. Biotechnol. Prog. (2000) [Pubmed]
  20. Mutants of Escherichia coli producing pyrroloquinoline quinone. Biville, F., Turlin, E., Gasser, F. J. Gen. Microbiol. (1991) [Pubmed]
 
WikiGenes - Universities