Disease relevance of glpD

• Aerobic sn-glycerol 3-phosphate dehydrogenase, encoded by the glpD gene of Escherichia coli, is a cytoplasmic membrane-associated respiratory enzyme [1].
• An inverted repeat preceding the Bacillus subtilis glpD gene is a conditional terminator of transcription [2].

High impact information on glpD

• In this case, the relative sensitivity of the glp operons to catabolite repression was glpTQ greater than glpFK greater than glpACB greater than glpD [3].
• Comparison of the sequences of the repressor binding sites found in the glpTQ-glpACB control region with those operators previously described in the glpD operon allowed formulation of a consensus operator sequence which was the palindrome 5'-WATGTTCGWTAWC-GAACATW-3' (W is A or T) [4].
• Expression of glpD is induced by G3P in the presence of the glpP gene product [2].
• Full-length glpD transcripts can be detected only in glycerol-induced cells [2].
• The GlpD overexpression strain showed increased tolerance to ampicillin and ofloxacin, while a strain with glpD deleted had a decreased level of persisters in the stationary state [5].

Chemical compound and disease context of glpD

• The Bacillus subtilis glpD gene, encoding glycerol-3-phosphate (G3P) dehydrogenase, is preceded by a promoter and an inverted repeat which is located between the promoter and the glpD coding region [2].
• The Bacillus subtilis glpD gene encodes glycerol-3-phosphate (G3P) dehydrogenase [6].

Biological context of glpD

• The nucleotide sequence of glpD was determined [1].
• The position of glpD on this plasmid was determined by Tn1000 insertional inactivation experiments [7].
• In the presence of GlpP, the glpD mRNA is stabilized. glpD leader-lacZ fusions were integrated into the chromosomes of B. subtilis and Escherichia coli [8].
• The TS phenotype is due to temperature-dependent degradation of the glpD mRNA [8].
• Regulation of glpD and glpE gene expression by a cyclic AMP-cAMP receptor protein (cAMP-CRP) complex in Escherichia coli [9].

Other interactions of glpD

• The proposed translational start and reading frame of glpD were confirmed by determining the nucleotide sequence across the fusion joint of a glpD-lacZ translational fusion [1].
• The recombinant plasmid, designated pSH55, carried a 7.4-kilobase-pair HindIII fragment containing the glpD and glpR genes [7].
• In the double-reporter-gene system, the expression of glpD and glpE was found to be positively regulated by cAMP-CRP [9].
• Effect of glpT and glpD mutations on expression of the phoA gene in Escherichia coli [10].

References