Disease relevance of cpxA

• The Cpx proteins of Escherichia coli K12. Immunologic detection of the chromosomal cpxA gene product [1].
• Furthermore, cpxA* mutations suppress the toxicity conferred by the LamB-LacZ hybrid protein, which exerts its effects in the cytoplasm, sequestered from DegP [2].
• Integration of multiple domains in a two-component sensor protein: the Bordetella pertussis BvgAS phosphorelay [3].

High impact information on cpxA

• Defined null mutations in cpxA or the gene encoding its cognate response regulator, cpxR, decrease transcription from degP [4].
• Previous studies described lacZ'- and cat'-'cpxA fusion genes whose expression restored to normal all the phenotypic defects associated with cpxA mutations (Albin, R., and Silverman, P. M. (1984) Mol. Gen. Genet. 197, 272-279) [1].
• The polypeptide showed temperature-sensitive accumulation in a strain carrying both the cpxA2[Ts] and cpxB1 alleles and accumulated to a level higher than normal in cells that carried a high-copy number, cpxA+ plasmid [1].
• Using this information, we constructed a fusion gene containing the same 241 cpxA codons preceded by 1007 codons of beta-galactosidase [1].
• The eup genetic locus of Escherichia coli and its role in H+/solute symport [5].

Chemical compound and disease context of cpxA

• L-serine degradation in Escherichia coli K-12: directly isolated ssd mutants and their intragenic revertants [6].

Biological context of cpxA

• The DNA sequence data also indicate that cpxA is the 3' gene of an operon [7].
• The complete cpxA nucleotide sequence, reported here, predicted that CpxA contains two extended, hydrophobic segments in its amino-terminal half and could therefore be a membrane protein [7].
• When transferred by P1 transduction to a cpxB11 Hfr strain, each of the mutations conferred the Tra- and Ilv- phenotypes characteristic of earlier cpxA mutants [8].
• Using methods that ensured independent mutations in the cpxA region of the chromosome, we isolated six new amikacin-resistant mutants following nitrosoguanidine mutagenesis [8].
• Aberrant cell division and random FtsZ ring positioning in Escherichia coli cpxA* mutants [9].

Anatomical context of cpxA

• Involvement of the Escherichia coli O157:H7(pO157) ecf operon and lipid A myristoyl transferase activity in bacterial survival in the bovine gastrointestinal tract and bacterial persistence in farm water troughs [10].

Associations of cpxA with chemical compounds

• The amikacin resistance phenotype of the cpxA mutant was inseparable by recombination from the cpxA mutant phenotype (inability to grow at 41 degrees C without exogenous isoleucine and valine) and was recessive to the cpxA+ allele of a recombinant plasmid [8].
• A mutation at the ssd locus resulted in elevated L-serine deaminase activity, inability to grow with succinate as the carbon source, and inability to grow anaerobic conditions [11].

Other interactions of cpxA

• While a functional degP gene is required for full cpxA*-mediated suppression of both toxic envelope proteins, residual suppression is seen in cpxA* degP::Tn10 double mutants [2].
• Two additional insertions were identified in genes sharing homology with the cpxA and damX genes from Escherichia coli K-12, and two uncharacterized invasion-deficient mutants were nonmotile [12].
• We show that the negative effect of the cpxA mutation on biofilm formation results from a decreased transcription of the curlin encoding csgA gene [13].
• Two transposon mutants with increased susceptibility to POA were found to harbor mutations in acnA encoding aconitase-1 and ygiY encoding a putative two-component sensor protein [14].

Analytical, diagnostic and therapeutic context of cpxA

• A 2.1-kb segment upstream from cpxA was amplified by polymerase chain reaction, cloned and sequenced [15].

References