Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

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Gene Review:

mcrC - 5-methylcytosine-specific restriction...

Escherichia coli str. K-12 substr. MG1655

Synonyms: ECK4335, JW5789

Ross, T.K. et al., Stroup, D. et al., Krüger, T. et al., Raleigh, E.A., Panne, D. et al., et al.

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Disease relevance of mcrC

The polypeptide encoded by the mcrC gene has been identified in Methanococcus vannielii by immunoblotting using rabbit antibodies raised against the product of a lacZ-mcrC gene fusion synthesized and purified from Escherichia coli [1].

High impact information on mcrC

Two gene products, McrBL and McrBs, are produced from the mcrB gene and one, McrC, from the mcrC gene [2].
The mcrC gene product contains a leucine heptad repeat that could play a role in protein-protein interactions [3].
It causes premature termination of mcrB gene product synthesis and reduces the level of mcrC gene expression [4].
Within the McrB region of the chromosome is the mcrB gene, which encodes a protein of 51 kilodaltons (kDa) (T. K. Ross, E. C. Achberger, and H. D. Braymer, Gene 61:277-289, 1987), and the mcrC gene, the product of which is 39 kDa (T. K. Ross, E. C. Achberger, and H. D. Braymer, Mol. Gen. Genet., in press) [5].

Biological context of mcrC

The deduced amino acid sequence was used to identify two open reading frames specifying peptides of 455 and 348 amino acids, corresponding to the products of the mcrB and mcrC genes, respectively [5].
In vitro expression of recombinant plasmids carrying all or portions of the mcrBC region revealed that the mcrB and mcrC genes are organized as an operon [4].

Other interactions of mcrC

Genetic and sequence analysis shows that the locus actually comprises two genes, mcrB and mcrC [6].

Analytical, diagnostic and therapeutic context of mcrC

The mcrC gene product (gpmcrC) was located in both the supernatant and pellet fractions after centrifugation of Mc. vannielii cell extracts for 2 h at 100,000 x g [1].

References

Identification of the mcrC gene product in Methanococcus vannielii. Stroup, D., Reeve, J.N. FEMS Microbiol. Lett. (1993) [Pubmed]
McrBs, a modulator peptide for McrBC activity. Panne, D., Raleigh, E.A., Bickle, T.A. EMBO J. (1998) [Pubmed]
Organization and function of the mcrBC genes of Escherichia coli K-12. Raleigh, E.A. Mol. Microbiol. (1992) [Pubmed]
Characterization of the mcrBC region of Escherichia coli K-12 wild-type and mutant strains. Krüger, T., Grund, C., Wild, C., Noyer-Weidner, M. Gene (1992) [Pubmed]
Nucleotide sequence of the McrB region of Escherichia coli K-12 and evidence for two independent translational initiation sites at the mcrB locus. Ross, T.K., Achberger, E.C., Braymer, H.D. J. Bacteriol. (1989) [Pubmed]
Genetic and sequence organization of the mcrBC locus of Escherichia coli K-12. Dila, D., Sutherland, E., Moran, L., Slatko, B., Raleigh, E.A. J. Bacteriol. (1990) [Pubmed]

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